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324726 Endoglycosidase F2, Elizabethkingia meningosepticum, Recombinant, E. coli

Endoglycosidase F2, Elizabethkingia meningosepticum, Recombinant, E. coli cleaves asparagine-linked or free oligomannose and biantennary complex oligosaccharides. It is not active above pH 6.0.

Endoglycosidase F2, Elizabethkingia meningosepticum, Recombinant, E. coli: のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。

同義語: Endo-β-N-acetylglucosaminidase F2, Endo F2

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324726
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概要

Replacement Information

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      		 樹脂ｱﾝﾌﾟﾙ 200 miu
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      Description
      OverviewRecombinant, Elizabethkingia meningosepticum endoglycosidase F2 expressed in E. coli. Cleaves asparagine-linked or free oligomannose and biantennary complex oligosaccharides from glycoproteins. It cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine. Less sensitive to protein conformation than N-Glycosidase F (Cat. No. 362185) and therefore is more suitable for deglycosylation of native proteins. This enzyme is not active above pH 6.0.
      Note: 1 mU = 1 milliunit.
      Catalogue Number324726
      Brand Family Calbiochem®
      SynonymsEndo-β-N-acetylglucosaminidase F2, Endo F2
      References
      ReferencesReddy, A., et al. 1998. Glycobiology 8, 633.
      Tarentino, A.L., and Plummer, T.H. 1994. Methods Enzymol. 230, 44.
      Tarentino, A.L., et al. 1993. J. Biol. Chem. 268, 9702.
      Trimble, R.B., and Tarentino, A.L. 1991. J. Biol. Chem. 266, 1646.
      Product Information
      Activity≥5 units/ml
      Unit of DefinitionOne unit is defined as the amount of enzyme that will release N-linked oligosaccharides from 1 µmol porcine fibrinogen per min at 37°C, pH 4.5.
      EC number3.2.1.96
      FormLiquid
      FormulationIn 25 mM NaCl, 10 mM sodium acetate buffer, pH 4.5.
      Quality LevelMQ100
      Applications
      Biological Information
      Specific Activity≥20 units/mg protein
      Physicochemical Information
      ContaminantsN-acetylglucosaminidase, α- and β-galactosidase, α-mannosidase, neuraminidases, proteases: none detected
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      324726-200MIUCN 04055977216004

      Documentation

      Endoglycosidase F2, Elizabethkingia meningosepticum, Recombinant, E. coli (M)SDS

      タイトル

      英語版製品安全データシート（(M)SDS） 

      Endoglycosidase F2, Elizabethkingia meningosepticum, Recombinant, E. coli 試験成績書（CoA）

      タイトルロット番号
      324726

      参考資料

      参考資料の概要
      Reddy, A., et al. 1998. Glycobiology 8, 633.
      Tarentino, A.L., and Plummer, T.H. 1994. Methods Enzymol. 230, 44.
      Tarentino, A.L., et al. 1993. J. Biol. Chem. 268, 9702.
      Trimble, R.B., and Tarentino, A.L. 1991. J. Biol. Chem. 266, 1646.
      データシート

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision11-September-2007 RFH
      SynonymsEndo-β-N-acetylglucosaminidase F2, Endo F2
      DescriptionRecombinant, Elizabethkingia meningosepticum endoglycosidase F2 expressed in E. coli. Cleaves asparagine-linked or free oligomannose and biantennary complex oligosaccharides from glycoproteins. It cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine. Less sensitive to protein conformation than N-Glycosidase F (Cat. No. 362185) and therefore, is more suitable for deglycosylation of native proteins.
      FormLiquid
      FormulationIn 25 mM NaCl, 10 mM sodium acetate buffer, pH 4.5.
      Recommended reaction conditions50 mM sodium acetate buffer, pH 4.5. (Note: enzyme is not active above pH 6.0)
      EC number3.2.1.96
      ContaminantsN-acetylglucosaminidase, α- and β-galactosidase, α-mannosidase, neuraminidases, proteases: none detected
      Specific activity≥20 units/mg protein
      Activity≥5 units/ml
      Unit definitionOne unit is defined as the amount of enzyme that will release N-linked oligosaccharides from 1 µmol porcine fibrinogen per min at 37°C, pH 4.5.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesReddy, A., et al. 1998. Glycobiology 8, 633.
      Tarentino, A.L., and Plummer, T.H. 1994. Methods Enzymol. 230, 44.
      Tarentino, A.L., et al. 1993. J. Biol. Chem. 268, 9702.
      Trimble, R.B., and Tarentino, A.L. 1991. J. Biol. Chem. 266, 1646.