マルチスクリーン（MultiScreen）HTS Elispot用フィルタープレート
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参考資料
| 参考資料の概要 | アプリケーション |
|---|---|
| Simultaneous detrection of multiple cytokines in ELISPOT assays. Palzer S. Bailey T., Hartnett C., Grant A., Tsang M., Kalyuzhny AE. Methods Mol Biol. 2005; 305:273-88 2005 | ELISPOT Assays |
| The Granzyme B ELISPOT assay: an alternative to the 51Cr-release assay for monitoring cell-mediated cytotoxicity Kimberly Shafer-Weaver, Thomas Sayers, Susan Strobl, Eric Derby, Tracy Ulderich, Michael Baseler and Anatoli Malyguine Journal of Translational Medicine 2003, 1:14 doi:10.1186/1479-5876-1-14 2003 | ELISPOT Assays |
| Protein kinase C [beta]II activation induces angiotensin converting enzyme expression in neonatal rat cardiomyocytes. Yuke Zhang, Laura J. Bloem, Lan Yu, Thomas B. Estridge, Philip W. Iversen, Christine E. McDonald, James P. Schrementi, XuShan Wang, Chris J. Vlahos and Jian Wang Cardiovascular Research 57 (1): 139-146 2003 | Enzyme Assays |
| Regional, but not systemic recruitment/expansion of dendritic cells by a pluronic-formulated Flt3-ligand plasmid with vaccine adjuvant activity. Hongxun Sang, Vladimir M. Pisarev, Corey Munger, Simon Robinson, Jennifer Chavez, Lori Hatcher, Prahlad Parajuli, Yajun Guo and James E. Talmadge Vaccine 21 (21-22): 3019-3029 2003 | ELISPOT Assays |
| CD40 ligand (CD154) improves the durability of respiratory syncytial virus DNA vaccination in BALB/c mice. Jennifer L. Harcourt, Michael P. Brown, Larry J. Anderson and Ralph A. Tripp Vaccine 21 (21-22): 2964-2979 2003 | ELISPOT Assays |
| Rectal immunization of mice with hepatitis A vaccine induces stronger systemic and local immune responses than parenteral immunization. Leslie Ann Mitchell and Eithan Galun Vaccine 21 (13-14): 1527-1538 2003 | ELISPOT Assays |
| A novel multivalent human CTL peptide construct elicits robust cellular immune responses in HLA-A*0201 transgenic mice: implications for HTLV-1 vaccine design. Roshni Sundaram, Yiping Sun, Christopher M. Walker, Francois A. Lemonnier, Steven Jacobson and Pravin T. P. Kaumaya Vaccine 21 (21-22): 2767-2781 2003 | ELISPOT Assays |
| Effective induction of CD8+ T-cell response using CpG oligodeoxynucleotides and HER-2/neu-derived peptide co-encapsulated in liposomes. Wai Ming Li, Wieslawa H. Dragowska, Marcel B. Bally and Marie-Paule Schutze-Redelmeier Vaccine 21 (23): 3319-3329 2003 | ELISPOT Assays |
| The role of lipopolysaccharide in T-cell responses following DNA vaccination. William G. Hawkins, Jiri Trcka, Neil Segal, Nathalie E. Blachere, Jason S. Gold, Yoichi Moroi, Wilbur B. Bowne, Jonathan J. Lewis, Jedd D. Wolchok and Alan N. Houghton Vaccine 21 (13-14): 1548-1553 2003 | ELISPOT Assays |
| A quantitive, highly sensitive cell-based infectivity assay for mouse scrapie prions. P.-C. Klöhn, L. Stoltze, E. Flechsig, M. Enari, and C. Weissmann PNAS 100 (D6920): 11666-11671 2003 | ELISPOT Assays |
FAQ（よくある質問と回答）
| 質問 | 回答 |
|---|---|
| When performing an ELISpot assay should MultiScreen plates with Immobilon-P membrane (MAIP and MSIP) be initially pre-wet with alcohol(methanol/ethanol)? | Yes. Wetting the membrane with 15 µL alcohol followed by two to three washes with sterile PBS will allow the antibody more intimate contact with the membrane. Do not vacuum the alcohol through the underdrain, rather it should be flicked out. Once the membrane is pre-wet with alcohol, do not allow it to dry for the duration of the assay. |
| Should MultiScreen MAHA and MSHA ELISpot plates with MCE membranes be initially pre-wet with alcohol or sterile buffer? | No. Neither is necessary. Methanol will dissolve the mixed-cellulose ester membrane. PBS is no longer needed. Membrane improvements ensure instant wetting of the membrane. |
| We are looking to coat MultiScreen ELISpot plates with primary antibody and store them. Do you have a recommended protocol? | After coating with primary antibody overnight at 4°C, wash twice with 150µL sterile Milli-Q® water. Dry plates thoroughly under sterile conditions and store for up to one week at 4°C. If longer storage is desired, the plates should be washed as previously described and then blocked (150µL/well media, 37°C, 2hr). Following blocking, wash 2x with 150µL sterile Milli-Q. Dry as before and store desiccated. Ab activity may diminish over time - especially in the event that the plates have not been blocked. Millipore has not determined shelf-life for either protocol as performance stability is likely to depend largely on the properties of the specific antibody under investigation. |
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