Millipore Sigma Vibrant Logo

317640 Dipeptidyl Peptidase IV (CD26), Porcine Kidney

Dipeptidyl Peptidase IV (CD26), Porcine Kidney: のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。

同義語: CD26, DPPIV

View This Product on Sigma-Aldrich
317640
価格＆在庫状況を照会

概要

Replacement Information

価格＆在庫状況

カタログ番号 在庫状況包装 Qty/Pk 価格 数量
317640-250MIUCN
在庫状況検索中…
—
現在国内在庫なし 現在国内在庫なし
現在国内在庫有り 
販売中止
在庫僅少
現在国内在庫あり
    Remaining : Will advise
      Remaining : Will advise
      注文対象外
      お問合せください
      Contact Customer Service

      		 樹脂ｱﾝﾌﾟﾙ 250 miu
      価格を検索中…
      価格が見つかりません
      Minimum Quantity needs to be mulitiple of
      Maximum Quantity is
      弊社照会 詳細を表示　
      値引
      ()
       
      —
      弊社照会
      		 —
      Description
      OverviewNative, DPP IV purified from porcine kidney (renal brush border of the proximal tubule). A serine exopeptidase dimer composed of two identical subunits of 110-130 kDa. Cleaves Xaa-Pro dipeptides from the N-terminus of oligo and polypeptides. DPPIV is involved in many cellular processes such as activation of cytokines, differentiation, T cell activation, and cell-matrix interactions. Inhibition of DPPIV has been reported to be an effective treatment for type II diabetes. MW: ~210000-260000 (unreduced).
      Catalogue Number317640
      Brand Family Calbiochem®
      SynonymsCD26, DPPIV
      References
      ReferencesBar, J., et al. 2003. Biol. Chem. 384, 1553.
      Engel, M., et al. 2003. Proc. Natl. Acad. Sci. USA 100, 5063.
      Ikehara, Y., et al. 1994. Methods. Enzymol. 244, 215.
      David, F., et al. 1993. J. Biol. Chem. 268, 17247.
      Thomsen, P.D., et al. 1993. Mamm. Genome 4, 604.
      Misumi, Y., et al. 1992. Biochim. Biophys. Acta. 1131, 333.
      Seidl, R., et al. 1991. Biol. Chem. Hoppe Seyler 372, 213.
      Checler, F., et al. 1985. J. Neurochem. 45, 1509.
      Imai, K., et al. 1983. J. Biochem. 93, 431.
      Kato, T., et al. 1979. Experientia. 35, 409.
      Kojima, K., et al. 1979. Anal. Biochem. 100, 43.
      Kato, T., et al. 1978. Biochem. Med. 19, 351.
      Kenny, A.J., et al. 1976. Biochem. J. 157, 169.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmole 7-(Gly-Pro)-amino-4-methylcoumarinamide per min at 37°C, pH 8.5.
      EC number3.4.14.5
      FormLiquid
      FormulationIn 20 mM Tris-HCl, 5 mM CaCl₂, 1 µM ZnCl₂, 0.05% NaN₃, pH 8.0.
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥90% by SDS-PAGE
      Specific Activity≥35 units/mg protein
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsDilute only the amount of enzyme needed for each assay. Upon thawing, do not re-freeze, store in the refrigerator (4°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      317640-250MIUCN 04055977216257

      Documentation

      Dipeptidyl Peptidase IV (CD26), Porcine Kidney (M)SDS

      タイトル

      英語版製品安全データシート（(M)SDS） 

      Dipeptidyl Peptidase IV (CD26), Porcine Kidney 試験成績書（CoA）

      タイトルロット番号
      317640

      参考資料

      参考資料の概要
      Bar, J., et al. 2003. Biol. Chem. 384, 1553.
      Engel, M., et al. 2003. Proc. Natl. Acad. Sci. USA 100, 5063.
      Ikehara, Y., et al. 1994. Methods. Enzymol. 244, 215.
      David, F., et al. 1993. J. Biol. Chem. 268, 17247.
      Thomsen, P.D., et al. 1993. Mamm. Genome 4, 604.
      Misumi, Y., et al. 1992. Biochim. Biophys. Acta. 1131, 333.
      Seidl, R., et al. 1991. Biol. Chem. Hoppe Seyler 372, 213.
      Checler, F., et al. 1985. J. Neurochem. 45, 1509.
      Imai, K., et al. 1983. J. Biochem. 93, 431.
      Kato, T., et al. 1979. Experientia. 35, 409.
      Kojima, K., et al. 1979. Anal. Biochem. 100, 43.
      Kato, T., et al. 1978. Biochem. Med. 19, 351.
      Kenny, A.J., et al. 1976. Biochem. J. 157, 169.
      データシート

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision19-June-2008 RFH
      SynonymsCD26, DPPIV
      DescriptionNative, DPP IV purified from porcine kidney (renal brush border of the proximal tubule). A serine exopeptidase dimer composed of two identical subunits of 110-130 kDa. Cleaves Xaa-Pro dipeptides from the N-terminus of oligo and polypeptides. DPPIV is involved in many cellular processes such as activation of cytokines, differentiation, T cell activation, and cell-matrix interactions. Inhibition of DPPIV has been reported to be an effective treatment for type II diabetes. MW: ~210000-260000 (unreduced).
      FormLiquid
      FormulationIn 20 mM Tris-HCl, 5 mM CaCl₂, 1 µM ZnCl₂, 0.05% NaN₃, pH 8.0.
      Concentration Label Please refer to vial label for lot-specific concentration
      Recommended reaction conditions
      Activity Assay The specific activity can be assayed using the synthetic substrate, 7-(Gly-Pro)-amino-4-methylcoumarinamide, and the cleavage product, 7-amino-4-methylcourmain (AMC). Both are fluorescent, but the excitation/emission spectra are different; at an excitation/emission setting of 380 nm/460 nm, only the AMC product is measured. For quantitative measurement an AMC standard curve should be included; the recommended range is 100-800 pmol. Alternatively, the activity can be measured using the colorimetric substrate, H-Gly-Pro-pNA. The activity is then monitored at 405 nm over a period of time. A standard curve can be generated using 4-nitroaniline in the range of 5-100 nmol.
      EC number3.4.14.5
      Purity≥90% by SDS-PAGE
      Specific activity≥35 units/mg protein
      Unit definitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmole 7-(Gly-Pro)-amino-4-methylcoumarinamide per min at 37°C, pH 8.5.
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsDilute only the amount of enzyme needed for each assay. Upon thawing, do not re-freeze, store in the refrigerator (4°C).
      Toxicity Standard Handling
      ReferencesBar, J., et al. 2003. Biol. Chem. 384, 1553.
      Engel, M., et al. 2003. Proc. Natl. Acad. Sci. USA 100, 5063.
      Ikehara, Y., et al. 1994. Methods. Enzymol. 244, 215.
      David, F., et al. 1993. J. Biol. Chem. 268, 17247.
      Thomsen, P.D., et al. 1993. Mamm. Genome 4, 604.
      Misumi, Y., et al. 1992. Biochim. Biophys. Acta. 1131, 333.
      Seidl, R., et al. 1991. Biol. Chem. Hoppe Seyler 372, 213.
      Checler, F., et al. 1985. J. Neurochem. 45, 1509.
      Imai, K., et al. 1983. J. Biochem. 93, 431.
      Kato, T., et al. 1979. Experientia. 35, 409.
      Kojima, K., et al. 1979. Anal. Biochem. 100, 43.
      Kato, T., et al. 1978. Biochem. Med. 19, 351.
      Kenny, A.J., et al. 1976. Biochem. J. 157, 169.