数量	カタログ番号	注文内容	Qty/Pk	価格
			96-Well Plate

数量	カタログ番号	注文内容	Qty/Pk	価格
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

220477 Sigma-AldrichChitosanase, Streptomyces sp. N174

Chitosanase, Streptomyces sp. N174, CAS 51570-20-8, is a member of a new class of enzymes with antifungal properties. Efficiently catalyzes the hydrolysis of acetylated chitosan (0 to 60%).

Chitosanase, Streptomyces sp. N174: のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。

(M)SDS
試験成績書（CoA）
参考資料
Data Sheet

同義語: Chitosan N-acetylglucosaminohydrolase

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概要

Replacement Information

価格＆在庫状況

カタログ番号	在庫状況	包装	Qty/Pk	価格	数量
220477-10UCN	在庫状況検索中… — 現在国内在庫なし現在国内在庫有り販売中止在庫僅少現在国内在庫あり在庫を照会するにはログインしてください現在国内在庫有り現在国内在庫なし現在国内在庫有り Remaining : Will advise Remaining : Will advise 注文対象外お問合せください Contact Customer Service お問合せくださいお問合せください	樹脂ｱﾝﾌﾟﾙ	10 u	価格を検索中… 価格が見つかりません Minimum Quantity needs to be mulitiple of Maximum Quantity is 弊社照会詳細を表示　値引 () — 弊社照会納入価格を閲覧するにはログインしてください	在庫状況を照会 —	お気に入り/ショッピングリストに追加お気に入り/ショッピングリストに追加お気に入りに追加されました

Description
Overview	Native Chitosanase from Streptomyces species. A member of a new class of enzymes with antifungal properties that efficiently catalyzes the hydrolysis of acetylated chitosan (0 to 60%). Does not hydrolyze chitin or carboxylmethylcellulose. Acts by splitting the β1,4-glycosidic linkages of GlcNAc-GlcN (K_m = 29.3 µg/ml) and GlcNAc-GlcN. Its activity generally increases with decreasing acetylation of chitosan. Suitable for the efficient preparation of protoplasts of Mucor and other Zygomycetes, allowing the genetic manipulation of these fungi.
Catalogue Number	220477
Brand Family	Calbiochem®
Synonyms	Chitosan N-acetylglucosaminohydrolase

References
References	Boucher, I., et al. 1995. J. Biol. Chem. 270, 31077. Cuero, R.G., and Osuji, G.O. 1995. Food Addit. Contam. 12, 479. Fukamizo, T., et al. 1995. Biochem J. 311, 377. Li, T., et al. 1995. Plant Physiol. Biochem. 33, 599. Fukamizo, T., et al. 1994. Biochim. Biophys. Acta 1205, 183. Osswald, W.F., et al. 1994. Plant Cell Physiol. 35, 811.

Product Information
CAS number	51570-20-8
Unit of Definition	One unit is defined as the amount of enzyme that will release 1.0 µmol reducing sugars or glucosamine from chitosan per min at 37°C, pH 5.5.
EC number	3.2.1.132
Form	Liquid
Formulation	In 50 M sodium acetate, 50% glycerol, pH 5.0.
PI	7.5, temperature optimum 65°C for 10 minutes; recommended temperature 37°C
Quality Level	MQ100

Applications

Biological Information
Specific Activity	≥25 units/mg protein

Physicochemical Information
Contaminants	chitinolytic activity: ≤0.01%, proteases: <0.25 Azocasein U/ml (One Azocasein unit is defined as the amount of protease that will liberate sufficient pigment to increase the optical density at 366 nm by 0.01 after incubation for 30 minutes with Azocasein)

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Standard Handling
Storage	-20°C
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
カタログ番号	GTIN
220477-10UCN	04055977201123

Documentation

Chitosanase, Streptomyces sp. N174 (M)SDS

タイトル
英語版製品安全データシート（(M)SDS）

Chitosanase, Streptomyces sp. N174 試験成績書（CoA）

タイトル	ロット番号
220477	ロット番号を入力

参考資料

参考資料の概要
Boucher, I., et al. 1995. J. Biol. Chem. 270, 31077. Cuero, R.G., and Osuji, G.O. 1995. Food Addit. Contam. 12, 479. Fukamizo, T., et al. 1995. Biochem J. 311, 377. Li, T., et al. 1995. Plant Physiol. Biochem. 33, 599. Fukamizo, T., et al. 1994. Biochim. Biophys. Acta 1205, 183. Osswald, W.F., et al. 1994. Plant Cell Physiol. 35, 811.

データシート

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	07-March-2019 JSW
Synonyms	Chitosan N-acetylglucosaminohydrolase
Description	Native Chitosanase from Streptomyces species. Chromatographically purified. Chitosanase is a member of a new class of enzymes with antifungal properties that efficiently catalyzes the hydrolysis of acetylated chitosan (0 to 60%). Does not hydrolyze chitin or carboxylmethyl cellulose. Acts by splitting the b1,4-glycosidic linkages of GlcN-GlcN (K_m = 29.3 µg/ml) and GlcNAc-GlcN. Its activity generally increases with decreasing acetylation of chitosan. Suitable for the efficient preparation of protoplasts of Mucor and other Zygomycetes, allowing the genetic manipulation of these fungi. Has an optimal pH of 5.5, an optimal temperature of 65°C for 10 min, and a recommended temperature of 37°C.
Form	Liquid
Formulation	In 50 M sodium acetate, 50% glycerol, pH 5.0.
CAS number	51570-20-8
EC number	3.2.1.132
Contaminants	chitinolytic activity: ≤0.01%, proteases: <0.25 Azocasein U/ml (One Azocasein unit is defined as the amount of protease that will liberate sufficient pigment to increase the optical density at 366 nm by 0.01 after incubation for 30 minutes with Azocasein)
Specific activity	≥25 units/mg protein
Unit definition	One unit is defined as the amount of enzyme that will release 1.0 µmol reducing sugars or glucosamine from chitosan per min at 37°C, pH 5.5.
Storage	Avoid freeze/thaw -20°C
Do Not Freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).
Toxicity	Standard Handling
References	Boucher, I., et al. 1995. J. Biol. Chem. 270, 31077. Cuero, R.G., and Osuji, G.O. 1995. Food Addit. Contam. 12, 479. Fukamizo, T., et al. 1995. Biochem J. 311, 377. Li, T., et al. 1995. Plant Physiol. Biochem. 33, 599. Fukamizo, T., et al. 1994. Biochim. Biophys. Acta 1205, 183. Osswald, W.F., et al. 1994. Plant Cell Physiol. 35, 811.

カテゴリー

Life Science Research > タンパク質と酵素 > Other Enzymes

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