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HTS185M ChemiSCREEN™ Membrane Preparation Recombinant Human EP2 Prostanoid Receptor

Human EP2 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.

ChemiSCREEN™ Membrane Preparation Recombinant Human EP2 Prostanoid Receptor: のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。
Host Cell: Chem-1 Uni Prot Number: P43116 Entrez Gene Number: NM_000956.2
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HTS185M
200 units  
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      概要

      Replacement Information

      主要スペック表

      SpeciesHost CellsProtein Target
      Human Chem-1EP2
      Description
      Catalogue NumberHTS185M
      Trade Name
      • ChemiScreen
      • Chemicon
      DescriptionChemiSCREEN™ Membrane Preparation Recombinant Human EP2 Prostanoid Receptor
      OverviewFull-length human PTGER2 cDNA encoding EP2
      Background InformationProstanoids bind to a family of 8 GPCRs to exert their biological effects (Narumiya and FitzGerald, 2001). The protanoid PGE2 causes pain, vasodilation, immunosuppression of T cells, bone resorption and promotion of carcinogenesis. Four related GPCRs, EP1, EP2, EP3 and EP4, each bind to PGE2, but the different G protein coupling status of each receptor leads to distinct biological effects. EP2 couples primarily to Gs to increase intracellular cAMP levels. Mice deficient in EP2 receptor showed impaired ovulation and fertilization, salt-sensitive hypertension (Kennedy et al., 1999). It has been shown that EP2 receptors are also involved in cancer associated immunodeficiency. Thus, genetic knockout of the EP2 receptor reduced tumor growth and prolonged survival in mice that had undergone isograft injection of MC26 or Lewis lung carcinoma cells (Yang et al., 2003). Millipore's EP2 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of agonists and antagonists of EP2. The membrane preparations exhibit a Kd of 14.52 nM for [3H]-PGE2. With 7.5 nM [3H]-PGE2, 10µg/well EP2 Membrane Prep typically yields greater than 3-fold signal-to-background ratio.
      References
      Product Information
      FormatMembranes
      PresentationLiquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives.
      Packaging method: Membranes protein were adjusted to the indicated concentration in packaging buffe, rapidly frozen, and stored at -80°C.
      Quality LevelMQ100
      Applications
      ApplicationHuman EP2 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
      Key Applications
      • Radioligand binding assay
      • GTPγS binding
      Application NotesRadioligand binding assay and GTPγS binding
      Biological Information
      Entrez Gene Number
      Gene Symbol
      • PTGER2
      • EP2
      GPCR ClassA
      Host CellsChem-1
      Protein TargetEP2
      Target Sub-FamilyProstanoid
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P43116 # Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. The subsequent raise in intracellular cAMP is responsible for the relaxing effect of this receptor on smooth muscle.
      SIZE: 358 amino acids; 39761 Da
      SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
      TISSUE SPECIFICITY: Placenta and lung.
      SIMILARITY: SwissProt: P43116 ## Belongs to the G-protein coupled receptor 1 family.
      Incubation ConditionsMembranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, a GF/C 96-well filter plate is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4, 500mM NaCl. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.
      Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, filtered and stored at 4°C.
      Radioligand: [3H]-PGE2 (Perkin Elmer#: NET-428)
      Wash Buffer: 50 mM Hepes, pH 7.4, 500mM NaCl, filtered and stored at 4°C.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Table 1. Signal:background and specific binding values obtained in a competition binding assay with varying amounts of EP2 Receptor membrane prep.
      Product Usage Statements
      10 µg/well
      Signal:Background 3.60
      Specific Binding (cpm) 1146

      1 unit = 10 µg
      Bmax for [3H]-PGE2 binding: 4.38 pmol/mg protein
      Kd for [3H]-PGE2 binding: ~14.52 nM Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at –70°C. Product is stable for at least 6 months from the date of receipt when stored as directed. Do not freeze and thaw.
      Packaging Information
      Material Size200 units
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      HTS185M 04053252739262

      Documentation

      ChemiSCREEN™ Membrane Preparation Recombinant Human EP2 Prostanoid Receptor (M)SDS

      タイトル

      英語版製品安全データシート（(M)SDS） 

      ChemiSCREEN™ Membrane Preparation Recombinant Human EP2 Prostanoid Receptor 試験成績書（CoA）

      タイトルロット番号
      ChemiScreenTM MEMBRANE PREPARATION RECOMBINANT HUMAN EP2 PROSTANOID RECEPTOR - 2121326 2121326
      ChemiScreenTM MEMBRANE PREPARATION RECOMBINANT HUMAN EP2 PROSTANOID RECEPTOR - 2188005 2188005
      ChemiScreenTM MEMBRANE PREPARATION RECOMBINANT HUMAN EP2 PROSTANOID RECEPTOR - SC20170418 SC20170418

      参考資料

      参考資料の概要Pub Med ID
      Cancer-associated immunodeficiency and dendritic cell abnormalities mediated by the prostaglandin EP2 receptor
      Yang, Li, et al
      J Clin Invest, 111:727-35 (2003)  2003

      		12618527 12618527
      Salt-sensitive hypertension and reduced fertility in mice lacking the prostaglandin EP2 receptor.
      Kennedy, C R, et al.
      Nat. Med., 5: 217-20 (1999)  1999

      概要を表示する       		9930871 9930871

      カタログ

      タイトル
      Advancing cancer research: From hallmarks & biomarkers to tumor microenvironment progression

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      カテゴリー

      Life Science Research > Drug Discovery and Development > GPCR Cell Lines & Assays > GPCR Membrane Preparations
      Life Science Research > タンパク質と酵素 > GPCR Membrane Preparations