Millipore Sigma Vibrant Logo

HTS116M ChemiSCREEN™ Human M3 Muscarinic Acetylcholine Receptor Membrane Preparation

Human M3 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.

ChemiSCREEN™ Human M3 Muscarinic Acetylcholine Receptor Membrane Preparation: のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。
Host Cell: Chem-1 Uni Prot Number: P20309 Entrez Gene Number: NM_000740.2
View This Product on Sigma-Aldrich
HTS116M
200 units  
価格を検索中…
価格が見つかりません
Minimum Quantity needs to be mulitiple of
Maximum Quantity is
弊社照会 詳細を表示　
値引
()
 
—
弊社照会
—
現在国内在庫なし 現在国内在庫なし
現在国内在庫有り 
販売中止
在庫僅少
現在国内在庫あり
    Remaining : Will advise
      Remaining : Will advise
      注文対象外
      お問合せください
      Contact Customer Service

       

      お問合せください

      概要

      Replacement Information

      主要スペック表

      SpeciesHost CellsProtein Target
      Human Chem-1M3
      Description
      Catalogue NumberHTS116M
      Brand Family Chemicon®
      Trade Name
      • ChemiScreen
      • Chemicon
      DescriptionChemiSCREEN™ Human M3 Muscarinic Acetylcholine Receptor Membrane Preparation
      OverviewFull-length human CHRM3 cDNA encoding M3
      Background InformationThe muscarinic acetylcholine receptor (mAChR) family consists of five GPCRs that mediate some of the neurotransmission functions of acetylcholine in the CNS and the periphery. The M1, M3 and M5 receptors couple to Gq to mobilize intracellular calcium, whereas the M2 and M4 receptors couple to Gi/o to inhibit cAMP production (Caulfield and Birdsall, 1998). M3 is expressed prominently in smooth muscle, and plays a primary role in mediating mAChR agonist-induced contractility. Mice lacking M3 have dilated pupils, which indicates a role for M3 in regulating tone of the pupillary sphincter muscle. In addition, Msub3 plays a role in feeding, as indicated by the lean and hypophagic phenotype of M3-null mice (Wess, 2004). Chemicon's M3 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of M3 interactions with 4-DAMP. The membrane preparations exhibit a Kd of 0.72-1 nM for [3H]-4-DAMP. With 10 µg/well M3 Membrane Prep and 0.75 nM [3H]-4-DAMP, a greater than 4-fold signal-to-background ratio was obtained.
      References
      Product Information
      FormatMembranes
      PresentationLiquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA no preservatives.
      Packaging method: Membranes protein were adjusted to 0.5 mg/ml in 1 mL packaging buffer, rapidly frozen, and stored at -80°C.
      Quality LevelMQ100
      Applications
      ApplicationHuman M3 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
      Key Applications
      • Radioligand binding assay
      • GTPγS binding
      Application NotesRadioligand binding assay and GTPγS binding.
      Biological Information
      Entrez Gene Number
      Entrez Gene SummaryThe muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 3 controls smooth muscle contraction and its stimulation causes secretion of glandular tissue.
      Gene Symbol
      • CHRM3
      • HM3
      GPCR ClassA
      Host CellsChem-1
      Protein TargetM3
      Target Sub-FamilyAcetylcholine (muscarinic)
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P20309 # The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.
      SIZE: 590 amino acids; 66128 Da
      SUBCELLULAR LOCATION: Basolateral cell membrane; Multi-pass membrane protein.
      SIMILARITY: SwissProt: P20309 ## Belongs to the G-protein coupled receptor 1 family.
      Incubation ConditionsMembranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, a GF/C 96-well filter plate is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.
      Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, 0.2% BSA, filtered and stored at 4°C
      Radioligand: [3H]-4-DAMP (Perkin Elmer#:NET1040 )
      Wash Buffer: 50 mM Hepes, pH 7.4, 500mM NaCl , 0.1% BSA, filtered and stored at 4°C.
      One package contains enough membranes for at least 200 assays (units), where a unit is the amount of membrane that will yield greater than 4-fold signal:background with 3H labeled 4-DAMP at 0.75 nM
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information


      Signal:background and specific binding values obtained in a competition binding assay with varying amounts of M3 membrane prep:
      Product Usage Statements
      20 µg/well 10 µg/well
      Signal:Background 5.17 5.10
      Specific Binding (cpm) 1224 1030


      SPECIFICATIONS: 1 unit = 10 µg
      Bmax: 3.75 pmol/mg
      Kd: 0.86 nM Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain frozen at -70°C for up to 2 years. Do not freeze and thaw.
      Packaging Information
      Material Size200 units
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      HTS116M 04053252678844

      Documentation

      ChemiSCREEN™ Human M3 Muscarinic Acetylcholine Receptor Membrane Preparation (M)SDS

      タイトル

      英語版製品安全データシート（(M)SDS） 

      ChemiSCREEN™ Human M3 Muscarinic Acetylcholine Receptor Membrane Preparation 試験成績書（CoA）

      タイトルロット番号
      CHEMISCREENTM MEMBRANE PREPARATION RECOMBINANT HUMAN M3 SOMATOSTATIN RECEPTOR - 2165786 2165786
      CHEMISCREENTM MEMBRANE PREPARATION RECOMBINANT HUMAN M3 SOMATOSTATIN RECEPTOR - 2250867 2250867

      参考資料

      参考資料の概要Pub Med ID
      Muscarinic acetylcholine receptor knockout mice: novel phenotypes and clinical implications.
      Wess, Jürgen
      Annu. Rev. Pharmacol. Toxicol., 44: 423-50 (2004)  2004

      概要を表示する       		14744253 14744253
      International Union of Pharmacology. XVII. Classification of muscarinic acetylcholine receptors
      Caulfield, M P and Birdsall, N J
      Pharmacol Rev, 50:279-90 (1998)  1998

      		9647869 9647869

      データシート

      タイトル
      CHEMISCREENTM MEMBRANE PREPARATION RECOMBINANT HUMAN M3 SOMATOSTATIN RECEPTOR

      関連製品＆アプリケーション

      製品ファミリー

      ChemiSCREEN™ GPCR Stable Cell Lines, Membranes Preparation and Ready-to-Assay™ Frozen Cells

      Millipore offers a large selection of robust and reliable G-protein coupled receptor products, including Stable Cell Lines, Membrane Preparations, and Frozen Cells. See below for GPCR research tools.
      詳細はこちら >>

      関連製品: Brand Facete

      ChemiScreen™

      カテゴリー

      Life Science Research > Drug Discovery and Development > GPCR Cell Lines & Assays > GPCR Membrane Preparations
      Life Science Research > タンパク質と酵素 > GPCR Membrane Preparations