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Merck

14-448-M

Cdk2/Cyclin A Protein, active, 10 µg

Active, C-terminal His6-tagged human full length Cdk2 & N-terminal GST-tagged human full length Cyclin A, for use in Kinase Assays.

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この商品について

UNSPSC Code:
12352202
NACRES:
NA.41
eCl@ss:
32160405
Biological source:
human
Recombinant:
expressed in baculovirus infected Sf21 cells
Mol wt:
Mw 35 kDa
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biological source

human

Quality Segment

recombinant

expressed in baculovirus infected Sf21 cells

product line

Upstate®

specific activity

(For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.)

mol wt

Mw 35 kDa

purified by

(The cdk2 is purified by Ni2+/NTA agarose and then activated using CAK and repurified by Q Sepharose and Ni2+/NTA agarose. The cyclin A is purified using glutathioneagarose)

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable (kinase)

accession no.

 (FUNCTION: SwissProt: P24941 # Involved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2. ), NM_001798.2

NCBI accession no.

UniProt accession no.

General description

C-terminal His6-tagged human full length cdk2, and N-terminal GST-tagged human full length cyclin A
CDK2/cyclinA

Biochem/physiol Actions

Protein Target: CDK2/cyclinA
Target Sub-Family: CMGC

Packaging

Also available in 10 μg size and in bulk
Also available in 250μg size, please inquire for pricing and availability.

Physical form

The cdk2 is purified by Ni2+/NTA agarose and then activated using CAK and repurified by Q Sepharose and Ni2+/NTA agarose. The cyclin A is purified using glutathioneagarose

Preparation Note

6 months at -70°C

Analysis Note

routinely evaluated by phosphorylation of Histone H1

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Replaces: 14-407

Legal Information

Sepharose is a trademark of Cytiva
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Skin Sens. 1

保管分類

10 - Combustible liquids

wgk

WGK 1

flash_point_c

Not applicable



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