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219467 Cathepsin V, His•Tag®, Human, Recombinant, NSO Cells

Cathepsin V, His•Tag®, is a recombinant human procathepsin V (aa 18-334) fused at the N-terminus to a CD33 signal sequence (aa 1-16) & at C-terminus to a His•Tag® sequence & expressed in NSO cells.

Cathepsin V, His•Tag®, Human, Recombinant, NSO Cells: のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。

同義語: Cathepsin L2, Cathepsin U

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219467
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概要

Replacement Information

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      Description
      OverviewRecombinant, human procathepsin V (amino acids 18-334) fused at the N-terminus to a CD33 signal sequence (amino acids 1-16) and at the C-terminus to a His•Tag® sequence and expressed in NSO cells. Cat V is predominantly expressed in normal thymus, testis, and corneal epithelium. It is involved in the invariant chain degradation in thymic epithelial cells and its overexpression has been detected in myasthenia gravis. Abnormally wide spread expressions in mammary gland and colon have also been reported in breast and colonrectal carcinomas. Although it shows high amino acid identity with cathepsin L (Cat. No. 219382), these two cathepsins have very different tissue distribution and substrate specificity. As a result of glycosylation, this recombinant enzyme migrates ~40 kDa on SDS-PAGE under either reducing or non-reducing conditions. Requires activation prior to use.
      Catalogue Number219467
      Brand Family Calbiochem®
      SynonymsCathepsin L2, Cathepsin U
      References
      ReferencesTolosa, E., et al. 2003. J. Clin. Invest. 112, 517.
      Turk, V., et al. 2001. EMBO J. 20, 4629.
      Somoza, J.R., et al. 2000. Biochemistry 39, 12543.
      Bromme, D., et al. 1999. Biochemistry 38, 2377.
      Adachi, W., et al. 1998. Invest. Ophthalmol. Vis. Sci. 39, 1789.
      Santamaria, I., et al. 1998. Cancer Res. 58, 1624.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enzyme that will cleave 1 pmol the fluorescent substrate Z-LR-AMC per min at 25°C, pH 5.5.
      EC number3.4.22.43
      FormFrozen liquid
      FormulationIn 800 mM NaCl, 25 mM sodium acetate, 0.8 M NaCl, pH 5.0.
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥95% by SDS-PAGE
      Specific Activity≥300 units/µg
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      ContaminantsEndotoxins: ≤1.0 EU/µg Cathepsin V
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsTo activate rhCathepsin V, pre-incubate it at 20 µg/ml in 25 mM NaOAc, 100 mM NaCl, 5 mM DTT, pH 5.5 at room temperature. Following initial thaw aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      219467-10UGCN 04055977218497

      Documentation

      Cathepsin V, His•Tag®, Human, Recombinant, NSO Cells (M)SDS

      タイトル

      英語版製品安全データシート（(M)SDS） 

      Cathepsin V, His•Tag®, Human, Recombinant, NSO Cells 試験成績書（CoA）

      タイトルロット番号
      219467

      参考資料

      参考資料の概要
      Tolosa, E., et al. 2003. J. Clin. Invest. 112, 517.
      Turk, V., et al. 2001. EMBO J. 20, 4629.
      Somoza, J.R., et al. 2000. Biochemistry 39, 12543.
      Bromme, D., et al. 1999. Biochemistry 38, 2377.
      Adachi, W., et al. 1998. Invest. Ophthalmol. Vis. Sci. 39, 1789.
      Santamaria, I., et al. 1998. Cancer Res. 58, 1624.
      データシート

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision27-November-2008 RFH
      SynonymsCathepsin L2, Cathepsin U
      DescriptionRecombinant, human procathepsin V (amino acids 18-334) fused at the N-terminus to a CD33 signal sequence (amino acids 1-16) and at the C-terminus to a His•Tag® sequence and expressed in NSO cells. Cat V is predominantly expressed in normal thymus, testis, and corneal epithelium. It is involved in the invariant chain degradation in thymic epithelial cells and its overexpression has been detected in myasthenia gravis. Abnormally wide spread expressions in mammary gland and colon have also been reported in breast and colonrectal carcinomas. Although it shows high amino acid identity with cathepsin L (Cat. No. 219382), these two cathepsins have very different tissue distribution and substrate specificity. As a result of glycosylation, this recombinant enzyme migrates ~40 kDa on SDS-PAGE under either reducing or non-reducing conditions. Requires activation prior to use.
      FormFrozen liquid
      FormulationIn 800 mM NaCl, 25 mM sodium acetate, 0.8 M NaCl, pH 5.0.
      Concentration Label Please refer to vial label for lot-specific concentration
      EC number3.4.22.43
      Purity≥95% by SDS-PAGE
      ContaminantsEndotoxins: ≤1.0 EU/µg Cathepsin V
      Specific activity≥300 units/µg
      Unit definitionOne unit is defined as the amount of enzyme that will cleave 1 pmol the fluorescent substrate Z-LR-AMC per min at 25°C, pH 5.5.
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsTo activate rhCathepsin V, pre-incubate it at 20 µg/ml in 25 mM NaOAc, 100 mM NaCl, 5 mM DTT, pH 5.5 at room temperature. Following initial thaw aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesTolosa, E., et al. 2003. J. Clin. Invest. 112, 517.
      Turk, V., et al. 2001. EMBO J. 20, 4629.
      Somoza, J.R., et al. 2000. Biochemistry 39, 12543.
      Bromme, D., et al. 1999. Biochemistry 38, 2377.
      Adachi, W., et al. 1998. Invest. Ophthalmol. Vis. Sci. 39, 1789.
      Santamaria, I., et al. 1998. Cancer Res. 58, 1624.