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218783 Caspase-1, Human, Recombinant, E. coli

Caspase-1, Human, Recombinant, is expressed in E. coli and is fused at the N-terminus to a His•Tag® sequence.

Caspase-1, Human, Recombinant, E. coli: のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。

同義語: IL-1β Converting Enzyme, ICE

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218783
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概要

Replacement Information

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218783-3000UCN
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      		 樹脂ｱﾝﾌﾟﾙ 3000 u
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      Description
      OverviewRecombinant, human caspase-1 fused at the N-terminus to a His•Tag® sequence and expressed in E. coli. Useful for the study of enzyme regulation, cleavage of target substrates, and inhibitor screening. M.W. 10000 and 20000.
      Catalogue Number218783
      Brand Family Calbiochem®
      SynonymsIL-1β Converting Enzyme, ICE
      References
      ReferencesThornberry, N.A. 1994. Methods Enzymol. 244, 615.
      Thornberry, N.A., et al. 1992. Nature 356, 768.
      Product Information
      Activity≥50,000 units/ml
      Unit of DefinitionOne unit is defined as the amount of enzyme that will cleave 1.0 pmol Ac-YVAD-<i>p</i>NA (<a href ="/Products/ProductDisplay.asp→catNO=400025">Cat. No. 400025</a>) per min at 30°C, pH 7.4.
      FormLiquid
      FormulationIn 100 mM NaCl, 50 mM HEPES, 10 mM DTT, 1 mM EDTA, 10% glycerol, 0.5% CHAPS, pH 7.4.
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥90% by SDS-PAGE
      Specific Activity≥25,000 units/mg protein
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      218783-3000UCN 04055977218756

      Documentation

      Caspase-1, Human, Recombinant, E. coli (M)SDS

      タイトル

      英語版製品安全データシート（(M)SDS） 

      Caspase-1, Human, Recombinant, E. coli 試験成績書（CoA）

      タイトルロット番号
      218783

      参考資料

      参考資料の概要
      Thornberry, N.A. 1994. Methods Enzymol. 244, 615.
      Thornberry, N.A., et al. 1992. Nature 356, 768.

      カタログ

      タイトル
      Caspases and other Apoptosis Related Tools Brochure

      引用

      Title
    • Sinead M. Miggin, et al. (2007) NF-κB activation by the Toll-IL-1 receptor domain protein MyD88 adapter-like is regulated by caspase-1. Procedings of the National Academy of Science 104, 3372-3377.
      		•
      データシート

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision23-March-2009 JSW
      SynonymsIL-1β Converting Enzyme, ICE
      DescriptionRecombinant, human caspase-1 fused at the N-terminus to a His•Tag® sequence and expressed in E. coli. Useful for the study of enzyme regulation, cleavage of target substrates, and inhibitor screening. M.W. 10000 and 20000.
      FormLiquid
      FormulationIn 100 mM NaCl, 50 mM HEPES, 10 mM DTT, 1 mM EDTA, 10% glycerol, 0.5% CHAPS, pH 7.4.
      Purity≥90% by SDS-PAGE
      Specific activity≥25,000 units/mg protein
      Activity≥50,000 units/ml
      Unit definitionOne unit is defined as the amount of enzyme that will cleave 1.0 pmol Ac-YVAD-pNA (Cat. No. 400025) per min at 30°C, pH 7.4.
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesThornberry, N.A. 1994. Methods Enzymol. 244, 615.
      Thornberry, N.A., et al. 1992. Nature 356, 768.
      Citation
    • Sinead M. Miggin, et al. (2007) NF-κB activation by the Toll-IL-1 receptor domain protein MyD88 adapter-like is regulated by caspase-1. Procedings of the National Academy of Science 104, 3372-3377.
      		•