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208748 Calpain-1 Substrate, Fluorogenic - Calbiochem

An internally quenched fluorogenic substrate peptide derived from the calpain-1 cleavage site of α-spectrin.

Calpain-1 Substrate, Fluorogenic - Calbiochem: のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。

同義語: μ-Calpain Substrate, Fluorogenic, H-K(FAM)-EVY~GMMK(DABCYL)-OH

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208748
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概要

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      		 樹脂ｱﾝﾌﾟﾙ 2 mg
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      Description
      OverviewAn internally quenched fluorogenic substrate peptide derived from the calpain-1 cleavage site of α-spectrin. It is not recognized by trypsin or α-chymotrypsin and serves as a sensitive and specific substrate for calpain-1 (Km = 4.6 µM; kcat = 11 s-1). Cleavage occurs between Tyr-Gly residues and results in enhanced fluorescence.
      Catalogue Number208748
      Brand Family Calbiochem®
      Synonymsμ-Calpain Substrate, Fluorogenic, H-K(FAM)-EVY~GMMK(DABCYL)-OH
      References
      ReferencesMittoo, S., et al. 2003. Anal. Biochem. 319, 234.
      Product Information
      ATP CompetitiveN
      FormRed lyophilized solid
      FormulationSupplied as a trifluoroacetate salt.
      Hill FormulaC₇₉H₉₅N₁₃O₁₉S₂
      Chemical formulaC₇₉H₉₅N₁₃O₁₉S₂
      Hygroscopic Hygroscopic
      ReversibleN
      Quality LevelMQ100
      Applications
      Biological Information
      Primary TargetA sensitive and specific substrate for calpain-1
      Purity≥95% by HPLC
      Physicochemical Information
      Cell permeableN
      Emission max.
      Excitation max.
      Peptide SequenceH-Lys(FAM)-Glu-Val-Tyr~Gly-Met-Met-Lys(DABCYL)-OH
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage -20°C
      Protect from Light Protect from light
      Hygroscopic Hygroscopic
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      208748-2MGCN 04055977202830

      Documentation

      Calpain-1 Substrate, Fluorogenic - Calbiochem (M)SDS

      タイトル

      英語版製品安全データシート（(M)SDS） 

      Calpain-1 Substrate, Fluorogenic - Calbiochem 試験成績書（CoA）

      タイトルロット番号
      208748

      参考資料

      参考資料の概要
      Mittoo, S., et al. 2003. Anal. Biochem. 319, 234.
      データシート

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision28-May-2008 RFH
      Synonymsμ-Calpain Substrate, Fluorogenic, H-K(FAM)-EVY~GMMK(DABCYL)-OH
      DescriptionAn internally quenched fluorogenic substrate peptide derived from the calpain-1 cleavage site of α-spectrin. It is not recognized by trypsin or α-chymotrypsin and serves as a sensitive and specific substrate for calpain-1 (Km = 4.6 µM; kcat = 11 s-1). Cleavage occurs at the Tyr-Gly bond and results in enhanced fluorescence. Excitation max: ~490 nm; emission max: ~518 nm.
      FormRed lyophilized solid
      FormulationSupplied as a trifluoroacetate salt.
      Intert gas (Yes/No) Packaged under inert gas
      Recommended reaction conditions50 mM Tris-HCl, 50 mM NaCl, 5 mM β-mercaptoethanol, 5 mM CaCl2, 1 mM EDTA, 1 mM EGTA, and 1 µM substrate, pH 7.5.
      Chemical formulaC₇₉H₉₅N₁₃O₁₉S₂
      Peptide SequenceH-Lys(FAM)-Glu-Val-Tyr~Gly-Met-Met-Lys(DABCYL)-OH
      Purity≥95% by HPLC
      SolubilityDMSO (1 mg/ml)
      Storage Protect from light
      -20°C
      Hygroscopic
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Toxicity Standard Handling
      ReferencesMittoo, S., et al. 2003. Anal. Biochem. 319, 234.