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71205 Benzonase® Nuclease HC, Purity > 90%

Benzonase® Nuclease HC, Purity > 90%: のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。
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71205
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概要

Replacement Information

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71205-3CN
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      		 樹脂ｱﾝﾌﾟﾙ 25 ku
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      Description
      OverviewBenzonase® Nuclease is a genetically engineered endonuclease from Serratia marcescens. It degrades all forms of DNA and RNA (single stranded, double stranded, linear and circular) while having no proteolytic activity. It is effective over a wide range of conditions and possesses an exceptionally high specific activity. The enzyme completely digests nucleic acids to 5′-monophosphate terminated oligonucleotides 2 to 5 bases in length (below the hybridization limit), which is ideal for removal of nucleic acids from recombinant proteins, enabling compliance with FDA guidelines for nucleic acid contamination. The ability of Benzonase to rapidly hydrolyze nucleic acids makes the enzyme an excellent choice for viscosity reduction to reduce processing time and increase yields of protein. For example, the enzyme is compatible with BugBuster® and PopCulture® Protein Extraction Reagents and can therefore be added along with these reagents to eliminate viscosity and remove nucleic acids from E. coli extracts.

      The enzyme consists of two subunits of 30 kDa each. It is functional between pH 6 and 10 and from 0-42°C and requires 1-2 mM Mg2+ for activation. The enzyme is also active in the presence of ionic and non-ionic detergents, reducing agents, PMSF (1 mM), EDTA (1 mM) and urea (relative activity depends on specific conditions). Activity is inhibited by > 150 mM monovalent cations, > 100 mM phosphate, > 100 mM ammonium sulfate, or > 100 mM guanidine HCl.

      Benzonase Nuclease is available in ultrapure (> 99% by SDS-PAGE) and pure (> 90%) grades at a standard concentration of 25-29 U/µl and at a high concentration (HC) of 250 U/µl. Both preparations are free of detectable protease and have specific activity > 1 × 106 U/mg protein. The > 99% purity grade is tested for endotoxins and contains < 0.25 EU/1000 units. The product is supplied as a 0.2 µm filtered solution in 50% glycerol. Store at -20°C.

      Total endotoxin: < 0.25 EU/1,000 units. Purity: > 90% by SDS-PAGE.
      Catalogue Number71205
      Brand Family Novagen®
      References
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enzyme that causes a ΔA₂₆₀ of 1.0 in 30 minutes, which corresponds to complete digestion of 37 µg DNA.
      Quality LevelMQ200
      Applications
      Biological Information
      Purity> 90% by SDS-PAGE.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      71205-3CN 04055977271034

      Documentation

      Benzonase® Nuclease HC, Purity > 90% (M)SDS

      タイトル

      英語版製品安全データシート（(M)SDS） 

      Benzonase® Nuclease HC, Purity > 90% 試験成績書（CoA）

      タイトルロット番号
      71205

      カタログ

      タイトル
      Bulk Product Guide
      Protein Purification and Detection Tools
      安定したタンパク質抽出と効率的なサンプル調整に　Buster & Protein Extraction Kit シリーズとタンパク質調整関連製品

      技術情報

      タイトル
      Benzonase®endonuclease for improved primary recovery in an E. coli-based process for Fab production

      ユーザープロトコール

      タイトル
      TB261 Benzonase® Nuclease

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      カタログ番号 説明  
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      70746 Benzonase® Nuclease, Purity > 90% 価格＆在庫状況を表示
      71206 Benzonase® Nuclease HC, Purity > 99% 価格＆在庫状況を表示

      関連製品: Brand Facete

      Benzonase®

      カテゴリー

      Life Science Research > Protein Sample Preparation > Protein Extraction > Nucleases & Enhancers