数量	カタログ番号	注文内容	Qty/Pk	価格
			96-Well Plate

数量	カタログ番号	注文内容	Qty/Pk	価格
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

OP01 Sigma-AldrichAnti-v-H-Ras (Ab-1) Rat mAb (Y13-259)

This Anti-v-H-Ras (Ab-1) Rat mAb (Y13-259) is validated for use in Frozen Sections, Immunoblotting, IF, IP, Paraffin Sections, Neutralization Studies for the detection of v-H-Ras (Ab-1).

Anti-v-H-Ras (Ab-1) Rat mAb (Y13-259): のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。

(M)SDS
試験成績書（CoA）
参考資料
Data Sheet

お勧めの製品

概要

Replacement Information

主要スペック表

Species Reactivity	Host	Antibody Type
H, M, R	R	Monoclonal Antibody

価格＆在庫状況

カタログ番号	在庫状況	包装	Qty/Pk	価格	数量
OP01-100UGCN	在庫状況検索中… — 現在国内在庫なし現在国内在庫有り販売中止在庫僅少現在国内在庫あり在庫を照会するにはログインしてください現在国内在庫有り現在国内在庫なし現在国内在庫有り Remaining : Will advise Remaining : Will advise 注文対象外お問合せください Contact Customer Service お問合せくださいお問合せください	樹脂ｱﾝﾌﾟﾙ	100 μg	価格を検索中… 価格が見つかりません Minimum Quantity needs to be mulitiple of Maximum Quantity is 弊社照会詳細を表示　値引 () — 弊社照会納入価格を閲覧するにはログインしてください	在庫状況を照会 —	お気に入り/ショッピングリストに追加お気に入り/ショッピングリストに追加お気に入りに追加されました

Description
Overview	Recognizes both phosphorylated and nonphosphorylated forms of v-H-Ras, and v-K-Ras in SW480 and Y1 cells and normal skin tissue. Also recognizes H, K, and N-Ras human translated products.
Catalogue Number	OP01
Brand Family	Calbiochem®

References
References	Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112. Taparowsky, E., et al. 1983. Cell 34, 581. Ellis, R.W., et al. 1981. Nature 292, 506. Shih, T.Y., et al. 1980. Nature 287, 686.

Product Information
Form	Liquid
Formulation	In 0.05 M sodium phosphate buffer, 0.2% gelatin.
Positive control	SW480 or Y1 cells or normal skin tissue
Preservative	≤0.1% sodium azide
Quality Level	MQ100

Applications
Application References	Immunoprecipitation Furth, M.E., et al. 1982. J.Virol. 43, 294. Paraffin Sections, Immunoprecipitation, and Immunoblotting Furth, M.E., et al. 1987. Oncogene 1 47. Neutralization Signal, I.S., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 4725. Specificity Furth, M.E., et al. 1987. Oncogene 1, 47. Furth, M.E., et al. 1982. J. Virol. 43, 294.
Key Applications	Frozen Sections Immunoblotting (Western Blotting) Immunofluorescence Immunoprecipitation Neutralization Studies Paraffin Sections
Application Notes	Frozen Sections (5 µg/ml) Immunoblotting (2.5 µg/ml, colormetric or chemiluminescence) Immunofluorescence (2.5 µg/ml) Immunoprecipitation (use Cat. No. OP01A or 1 µg antibody per sample) Paraffin Sections (5 µg/ml, saponin or pepsin pre-treatment required) Neutralization Studies (see application references)
Application Comments	Broadly reactive with the phosphorylated and non-phosphorylated forms of v-H-Ras and v-K-Ras (see application references) and recognizes the p21 translational products of the H-, K-, and N-ras human genes (see application references). This antibody does not cross-react with rap. Neutralizes the biological and biochemical activities of H-, K-, and N-ras p21 of vertebrates by binding to residues Glu-63, Ser-65, Ala-66, Met-67, Gln-70, and Arg-73 (see application references). 15 µl v-H-ras (Ab-1) agarose conjugate (Cat. No. OP01A) is recommended to be used as an immunoconcentrator prior to immunoblot analysis of p21^ras with another antibody. To immunoprecipitate with the unconjugated antibody, use Protein A with a rabbit anti-rat IgG or Protein G PLUS with a goat anti-rat IgG. Protein A alone will not immunoprecipitate rat IgG. A doublet may be detected corresponding to ras protein with and without farnesyl group. Antibody should be titrated for optimal results in individual systems.

Biological Information
Immunogen	recombinant p21 Ras
Epitope	within amino acids 62-76
Clone	Y13-259
Host	Rat
Isotype	IgG₁
Species Reactivity	Human Mouse Rat
Antibody Type	Monoclonal Antibody
Concentration Label	Please refer to vial label for lot-specific concentration

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Standard Handling
Storage	+2°C to +8°C
Do not freeze	Yes

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
カタログ番号	GTIN
OP01-100UGCN	04055977209570

Documentation

Anti-v-H-Ras (Ab-1) Rat mAb (Y13-259) (M)SDS

タイトル
英語版製品安全データシート（(M)SDS）

Anti-v-H-Ras (Ab-1) Rat mAb (Y13-259) 試験成績書（CoA）

タイトル	ロット番号
OP01	ロット番号を入力

参考資料

参考資料の概要
Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112. Taparowsky, E., et al. 1983. Cell 34, 581. Ellis, R.W., et al. 1981. Nature 292, 506. Shih, T.Y., et al. 1980. Nature 287, 686.

データシート

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	01-October-2007 RFH
Application	Frozen Sections (5 µg/ml) Immunoblotting (2.5 µg/ml, colormetric or chemiluminescence) Immunofluorescence (2.5 µg/ml) Immunoprecipitation (use Cat. No. OP01A or 1 µg antibody per sample) Paraffin Sections (5 µg/ml, saponin or pepsin pre-treatment required) Neutralization Studies (see application references)
Description	Purified rat monoclonal antibody generated by immunizing Sprague Dawlay rats with the specified immunogen and fusing splenocytes with Y3Ag1.2.3 rat myeloma cells. Recognizes the ~21 kDa Ras protein (see comments).
Background	Oncogene sequences originally isolated from Harvey sarcoma virus v-H-ras and Kirsten sarcoma virus v-K-ras, both encode 21,000 dalton phosphoproteins (p21's) with guanine nucleotide binding activity. Human cellular homologues of v-H-ras and v-K-ras, designated c-H-ras and c-K-ras, respectively, and a related human oncogene, c-N-ras, each encode 21,000 dalton protein analogues to the gene products of their viral counterparts.
Host	Rat
Immunogen	recombinant p21 Ras
Epitope	within amino acids 62-76
Clone	Y13-259
Isotype	IgG₁
Species	human, mouse, rat
Positive control	SW480 or Y1 cells or normal skin tissue
Form	Liquid
Formulation	In 0.05 M sodium phosphate buffer, 0.2% gelatin.
Concentration Label	Please refer to vial label for lot-specific concentration
Preservative	≤0.1% sodium azide
Comments	Broadly reactive with the phosphorylated and non-phosphorylated forms of v-H-Ras and v-K-Ras (see application references) and recognizes the p21 translational products of the H-, K-, and N-ras human genes (see application references). This antibody does not cross-react with rap. Neutralizes the biological and biochemical activities of H-, K-, and N-ras p21 of vertebrates by binding to residues Glu-63, Ser-65, Ala-66, Met-67, Gln-70, and Arg-73 (see application references). 15 µl v-H-ras (Ab-1) agarose conjugate (Cat. No. OP01A) is recommended to be used as an immunoconcentrator prior to immunoblot analysis of p21^ras with another antibody. To immunoprecipitate with the unconjugated antibody, use Protein A with a rabbit anti-rat IgG or Protein G PLUS with a goat anti-rat IgG. Protein A alone will not immunoprecipitate rat IgG. A doublet may be detected corresponding to ras protein with and without farnesyl group. Antibody should be titrated for optimal results in individual systems.
Storage	+2°C to +8°C
Do Not Freeze	Yes
Toxicity	Standard Handling
References	Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112. Taparowsky, E., et al. 1983. Cell 34, 581. Ellis, R.W., et al. 1981. Nature 292, 506. Shih, T.Y., et al. 1980. Nature 287, 686.
Application references	Immunoprecipitation Furth, M.E., et al. 1982. J.Virol. 43, 294. Paraffin Sections, Immunoprecipitation, and Immunoblotting Furth, M.E., et al. 1987. Oncogene 1 47. Neutralization Signal, I.S., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 4725. Specificity Furth, M.E., et al. 1987. Oncogene 1, 47. Furth, M.E., et al. 1982. J. Virol. 43, 294.

Alternative Products

カタログ番号

説明

OP01A

Anti-v-H-Ras (Ab-1) Rat mAb (Y13-259) Agarose Conjugate

価格＆在庫状況を表示

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