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ABS194 Anti-phospho PDHE1-A type I (Ser300) Antibody

This Anti-phospho PDHE1-A type I (Ser300) Antibody is validated for use in WB, IC for the detection of phospho PDHE1-A type I (Ser300).

Anti-phospho PDHE1-A type I (Ser300) Antibody: のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。
Research Category: Signaling Gene Symbol: PDHA1, PHE1A UniProt Number: P08559
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ABS194
50 µg  
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      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, B, XnWB, ICCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABS194
      DescriptionAnti-phospho PDHE1-A type I (Ser300) Antibody
      Alternate Names
      • Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial
      • PDHE1-A type I
      Background InformationIn many organisms, the pyruvate dehydrogenase complex catalyzes the overall, irreversible conversion of pyruvate to acetyl-CoA and CO2 in the aerobic, energy-generating pathways, in addition to serving as a key regulator for cardiac substrate selection. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). PDH is regulated by both pyruvate dehydrogenase kinase (PDK)-mediated phosphorylation and feedback inhibition.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Dichloroacetate untreated and treated HEK 293 cell lysates
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-phospho PDHE1-A type I (Ser300) Antibody is validated for use in WB, IC for the detection of phospho PDHE1-A type I (Ser300).
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:500 dilution from a representative lot detected PDHE1-A type I in dichloroacetate untreated and treated HEK 293 cells.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human PDHE1-A type I phosphorylated at Ser300.
      EpitopePhosphorylated Ser300
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes PDHE1-A type I phosphorylated at Ser300.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Bovine
      • Xenopus
      Species Reactivity NoteDemonstrated to react with Human. Predicted to react with Mouse, Rat, Bovine, Zebra Fish, and Xenopus based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
      Gene Symbol
      • PDHA1
      • PHE1A
      Modifications
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).

      CATALYTIC ACTIVITY: Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2.

      COFACTOR: Thiamine pyrophosphate.

      ENZYME REGULATION: E1 activity is regulated by phosphorylation (inactivation) and dephosphorylation (activation) of the alpha subunit.

      SUBUNIT STRUCTURE: Tetramer of 2 alpha and 2 beta subunits.

      SUBCELLULAR LOCATION: Mitochondrion matrix.

      TISSUE SPECIFICITY: Ubiquitous.
      Molecular Weight~43 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in dichloroacetate untreated and treated HEK 293 cell lysates.

      Western Blot Analysis: 0.5 µg/mL of this antibody detected PDHE1-A type I on 10 µg of dichloroacetate untreated and treated HEK 293 cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      ABS194 04053252633966

      Documentation

      Anti-phospho PDHE1-A type I (Ser300) Antibody (M)SDS

      タイトル

      英語版製品安全データシート（(M)SDS） 

      Anti-phospho PDHE1-A type I (Ser300) Antibody 試験成績書（CoA）

      タイトルロット番号
      Anti-phospho PDHE1-A type I (Ser300) 2480941
      Anti-phospho PDHE1-A type I (Ser300) - 2356330 2356330
      Anti-phospho PDHE1-A type I (Ser300) - 2388993 2388993
      Anti-phospho PDHE1-A type I (Ser300) - 2397162 2397162
      Anti-phospho PDHE1-A type I (Ser300) - 2445031 2445031
      Anti-phospho PDHE1-A type I (Ser300) - 2271218 2271218
      Anti-phospho PDHE1-A type I (Ser300) - 2327007 2327007
      Anti-phospho PDHE1-A type I (Ser300) - 3216495 3216495
      Anti-phospho PDHE1-A type I (Ser300) - 3690731 3690731
      Anti-phospho PDHE1-A type I (Ser300) - 3807967 3807967
      Show more

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      カテゴリー

      Life Science Research > Antibodies and Assays > Primary Antibodies