MABS829
Anti-phospho-EGFR (Tyr1173) Antibody, clone 3E7.2
clone 3E7.2, from mouse
別名:
Epidermal growth factor receptor, EC: 2.7.10.1, Proto-oncogene c-ErbB1, Receptor tyrosine protein kinase erbB-1
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この商品について
UNSPSC Code:
12352203
NACRES:
NA.43
eCl@ss:
32160702
Clone:
3E7.2, monoclonal
Species reactivity:
human
Application:
ICC, WB
Citations:
-
biological source
mouse
Quality Segment
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
3E7.2, monoclonal
species reactivity
human
technique(s)
immunocytochemistry: suitable, western blot: suitable
isotype
IgG2bκ
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
phosphorylation (pTyr1173)
Gene Information
human ... EGFR(1956)
General description
Epidermal growth factor receptor (UniProt: P00533; also known as EC: 2.7.10.1; Proto-oncogene c-ErbB1, Receptor tyrosine protein kinase erbB-1) is encoded by the EGFR (also known as ERBB, ERBB1, HER1) gene (Gene ID: 1956) in human. EGFR is activated by binding of its specific ligands and transforms from a monomeric to active homodimeric form. In addition to forming homodimers, EGFR may pair with another member of the ErbB receptor family, such as ErbB2/Her2/neu, to create an activated heterodimer. EGFR dimerization stimulates its intrinsic intracellular protein-tyrosine kinase activity. As a result, autophosphorylation of five tyrosine residues (Y992, Y1045, Y1068, Y1148 and Y1173) occurs in the C-terminal domain. This autophosphorylation elicits downstream activation and signaling by several other proteins that associate with the phosphorylated tyrosines through their own phosphotyrosine-binding domains. EGFR activation results in at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLC gamma-PKC and STATs modules. These downstream events modulate several cell functions, including migration, adhesion, and proliferation. Endocytosis and inhibition of the activated EGFR by phosphatases constitute key regulatory mechanisms.
~200 kDa observed; 134.28 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Immunogen
KLH-conjugated linear peptide corresponding to 13 amino acids surrrounding Tyrosine 1173 of human epidermal growth factor receptor.
Application
Detect phospho EGFR using this mouse monoclonal Anti-phospho-EGFR (Tyr1173), clone 3E7.2, Cat. No. MABS829, used in Immunocytochemistry and Western Blotting.
Immunocytochemistry Analysis: 10 µg/mL from a representative lot detected phospho-EGFR (Tyr1173) in A431 cells treated with EGF.
Physical form
Format: Purified
Analysis Note
Evaluated by Western Blotting in A431 cells treated with EGF.
Western Blotting Analysis: A 1:5,000 dilution of this antibody detected phospho-EGFR (Tyr1173) in 10 µg lysate from A431 cells treated with EGF.
Western Blotting Analysis: A 1:5,000 dilution of this antibody detected phospho-EGFR (Tyr1173) in 10 µg lysate from A431 cells treated with EGF.
Other Notes
Concentration: Please refer to lot specific datasheet.
Replaces: 05-483
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保管分類
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
MABS829:
jan
試験成績書（COA）
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