数量	カタログ番号	注文内容	Qty/Pk	価格
			96-Well Plate

数量	カタログ番号	注文内容	Qty/Pk	価格
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

OP29 Sigma-AldrichAnti-p53 (Ab-3) (Mutant) Mouse mAb (PAb240)

This Anti-p53 (Ab-3) (Mutant) Mouse mAb (PAb240) is validated for use in FC, Frozen Sections, Gel Shift, Immunoblotting, IF, IP, Paraffin Sections for the detection of p53 (Ab-3) (Mutant).

Anti-p53 (Ab-3) (Mutant) Mouse mAb (PAb240): のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。

(M)SDS
試験成績書（CoA）
参考資料
カタログ
Data Sheet

お勧めの製品

概要

Replacement Information

主要スペック表

Species Reactivity	Host	Antibody Type
B, Ch, Ht, H, M, R	M	Monoclonal Antibody

価格＆在庫状況

カタログ番号	在庫状況	包装	Qty/Pk	価格	数量
OP29-100UGCN	在庫状況検索中… — 現在国内在庫なし現在国内在庫有り販売中止在庫僅少現在国内在庫あり在庫を照会するにはログインしてください現在国内在庫有り現在国内在庫なし現在国内在庫有り Remaining : Will advise Remaining : Will advise 注文対象外お問合せください Contact Customer Service お問合せくださいお問合せください	樹脂ｱﾝﾌﾟﾙ	100 μg	価格を検索中… 価格が見つかりません Minimum Quantity needs to be mulitiple of Maximum Quantity is 弊社照会詳細を表示　値引 () — 弊社照会納入価格を閲覧するにはログインしてください	在庫状況を照会 —	お気に入り/ショッピングリストに追加お気に入り/ショッピングリストに追加お気に入りに追加されました
OP29-200UGCN	在庫状況検索中… — 現在国内在庫なし現在国内在庫有り販売中止在庫僅少現在国内在庫あり在庫を照会するにはログインしてください現在国内在庫有り現在国内在庫なし現在国内在庫有り Remaining : Will advise Remaining : Will advise 注文対象外お問合せください Contact Customer Service お問合せくださいお問合せください	樹脂ｱﾝﾌﾟﾙ	200 μg	価格を検索中… 価格が見つかりません Minimum Quantity needs to be mulitiple of Maximum Quantity is 弊社照会詳細を表示　値引 () — 弊社照会納入価格を閲覧するにはログインしてください	在庫状況を照会 —	お気に入り/ショッピングリストに追加お気に入り/ショッピングリストに追加お気に入りに追加されました

Description
Overview	Recognizes the ~53 kDa mutant p53 protein under non-denaturing conditions by immunoprecipitation, immunofluorescence, and flow cytometry. Recognizes both mutant and wild-type p53 by immunoblotting and paraffin sections under denaturing conditions.
Catalogue Number	OP29
Brand Family	Calbiochem®

References
References	El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169. Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855. Barak, Y., et al. 1993. EMBO J. 12, 461. Kastan, M.B., et al. 1992. Cell 71, 587. Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491. Lane, D.P. 1992. Nature 358, 15. Kastan, M.B., et al. 1991. Cancer Res. 51, 6304.

Product Information
Form	Liquid
Formulation	In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Negative control	SK-OV-3 cells
Positive control	A431, Hs27 (wild-type p53), or SK-BR-3 cells or breast carcinoma tissue
Preservative	≤0.1% sodium azide
Quality Level	MQ100

Applications
Application References	Paraffin Sections Pezzella, F., et al. 1994. J. Clin. Pathol. 47, 592. Original Clone Gannon, J.V., et al. 1990. EMBO J. 9, 1595. Epitope Identification Stephen, C.W. and Lane, D.P., 1992. J. Mol. Biol. 225, 1. Immunofluorescence Bernardi, R., et al. 2004. Nat. Cell Biol. 6, 665.
Key Applications	Flow Cytometry Frozen Sections Gel Shift Immunoblotting (Western Blotting) Immunofluorescence Immunoprecipitation Paraffin Sections
Application Notes	Flow Cytometry (1-20 µg/ml) Frozen Sections (10 µg/ml) Gel Shift (see comments) Immunoblotting (5 µg/ml) Immunofluorescence (1-20 µg/ml, see application references) Immunoprecipitation (1 µg per sample) Paraffin Sections (see application references)
Application Comments	Under non-denaturing conditions (immunoprecipitation, immunofluorescence and frozen sections), Anti-p53 (Ab-3) does not recognize normal (wild-type) p53 protein; it recognizes an epitope exposed by activating mutations or denaturation. In denaturing protocols (immunoblotting and paraffin sections), Anti-p53 (Ab-3) will recognize both mutant and wild-type p53. Will not recognize to some p53 molecules with mutations in the RHSVV epitope, but will react to TFIIIA, which has the RHSVV epitope. For gel shift assay, use Cat. No. OP29L and resuspend in 100 µl buffer. Antibody should be titrated for optimal results in individual systems.

Biological Information
Immunogen	a recombinant protein consisting of amino acids 14-389 of p53 fused to β-galactosidase
Immunogen	Human
Epitope	within amino acids 213-217
Clone	PAb240
Host	Mouse
Isotype	IgG₁
Species Reactivity	Bovine Chicken Hamster Human Mouse Rat
Antibody Type	Monoclonal Antibody
Concentration Label	Please refer to vial label for lot-specific concentration

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Standard Handling
Storage	+2°C to +8°C
Do not freeze	Yes

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
カタログ番号	GTIN
OP29-100UGCN	07790788053888
OP29-200UGCN	04055977208429

Documentation

Anti-p53 (Ab-3) (Mutant) Mouse mAb (PAb240) (M)SDS

タイトル
英語版製品安全データシート（(M)SDS）

Anti-p53 (Ab-3) (Mutant) Mouse mAb (PAb240) 試験成績書（CoA）

タイトル	ロット番号
OP29	ロット番号を入力

参考資料

参考資料の概要
El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169. Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855. Barak, Y., et al. 1993. EMBO J. 12, 461. Kastan, M.B., et al. 1992. Cell 71, 587. Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491. Lane, D.P. 1992. Nature 358, 15. Kastan, M.B., et al. 1991. Cancer Res. 51, 6304.

カタログ

タイトル
Caspases and other Apoptosis Related Tools Brochure

データシート

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	02-October-2007 RFH
Application	Flow Cytometry (1-20 µg/ml) Frozen Sections (10 µg/ml) Gel Shift (see comments) Immunoblotting (5 µg/ml) Immunofluorescence (1-20 µg/ml, see application references) Immunoprecipitation (1 µg per sample) Paraffin Sections (see application references)
Description	Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2 mouse myeloma cells (see application references). Recognizes the ~53 kDa mutant p53 protein under non-denaturing conditions. Recognizes both the mutant and the wild-type p53 protein under denaturing conditions.
Background	The human p53 tumor suppressor gene encodes a 393 amino acid phosphoprotein that exhibits sequence-specific DNA binding and directly interacts with various cellular and viral proteins. p53 is the most commonly mutated gene in human cancer, with the majority of the mutations being amino acid substitutions. The normal function of p53 is to effect cell cycle arrest at the G1 and G2 checkpoints in response to DNA damage. This checkpoint function is executed by accumulation of p53 followed by induction of the GADD45, WAF1 and MDM2 genes. The current model of p53 function postulates that p53 senses DNA damage and arrests the cell cycle in either the G1 or G2 phases to allow DNA repair to take place. If repair is not successful, p53 initiates programmed cell death, thus preventing the propagation of genetic defects to successive generations of cells.
Host	Mouse
Immunogen species	Human
Immunogen	a recombinant protein consisting of amino acids 14-389 of p53 fused to β-galactosidase
Epitope	within amino acids 213-217
Clone	PAb240
Isotype	IgG₁
Species	not Xenopus, bovine, chicken, hamster, human, mouse, rat
Positive control	A431, Hs27 (wild-type p53), or SK-BR-3 cells or breast carcinoma tissue
Negative control	SK-OV-3 cells
Form	Liquid
Formulation	In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Concentration Label	Please refer to vial label for lot-specific concentration
Preservative	≤0.1% sodium azide
Comments	Under non-denaturing conditions (immunoprecipitation, immunofluorescence and frozen sections), Anti-p53 (Ab-3) does not recognize normal (wild-type) p53 protein; it recognizes an epitope exposed by activating mutations or denaturation. In denaturing protocols (immunoblotting and paraffin sections), Anti-p53 (Ab-3) will recognize both mutant and wild-type p53. Will not recognize to some p53 molecules with mutations in the RHSVV epitope, but will react to TFIIIA, which has the RHSVV epitope. For gel shift assay, use Cat. No. OP29L and resuspend in 100 µl buffer. Antibody should be titrated for optimal results in individual systems.
Storage	+2°C to +8°C
Do Not Freeze	Yes
Toxicity	Standard Handling
References	El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169. Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855. Barak, Y., et al. 1993. EMBO J. 12, 461. Kastan, M.B., et al. 1992. Cell 71, 587. Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491. Lane, D.P. 1992. Nature 358, 15. Kastan, M.B., et al. 1991. Cancer Res. 51, 6304.
Application references	Paraffin Sections Pezzella, F., et al. 1994. J. Clin. Pathol. 47, 592. Original Clone Gannon, J.V., et al. 1990. EMBO J. 9, 1595. Epitope Identification Stephen, C.W. and Lane, D.P., 1992. J. Mol. Biol. 225, 1. Immunofluorescence Bernardi, R., et al. 2004. Nat. Cell Biol. 6, 665.

Alternative Products

カタログ番号

説明

OP29L

Anti-p53 (Ab-3) (Mutant) Mouse mAb (PAb240)

価格＆在庫状況を表示

カテゴリー

Life Science Research > Antibodies and Assays > Primary Antibodies

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