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ABS310 Anti-hCE-1 Antibody

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ABS310
100 µL  
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      概要

      Replacement Information

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      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbSerumPolyclonal Antibody
      Description
      Catalogue NumberABS310
      DescriptionAnti-hCE-1 Antibody
      Alternate Names
      • Carboxylesterase 1
      • Liver carboxylesterase 1
      • Acyl-coenzyme A:cholesterol acyltransferase
      • ACAT
      • Brain carboxylesterase hBr1
      • CE-1
      • hCE-1
      • Cocaine carboxylesterase
      • Egasyn
      • HMSE
      • Methylumbelliferyl-acetate deacetylase 1
      • Monocyte/macrophage serine esterase
      • Retinyl ester hydrolase
      • REH
      • Serine esterase 1
      • Triacylglycerol hydrolase
      • TGH
      Background InformationCarboxylesterase 1 (hCE-1) is also known as Liver carboxylesterase 1, Acyl-coenzyme A:cholesterol acyltransferase, (ACAT), Cocaine carboxylesterase, Methylumbelliferyl-acetate deacetylase 1, Monocyte/macrophage serine esterase, Retinyl ester hydrolase, (REH), Serine esterase 1, and Triacylglycerol hydrolase (TGH). hCE-1 is involved in: the detoxification of xenobiotics and the activation of ester and amide prodrugs. hCE-1 functions to hydrolize the methyl ester group of cocaine to form benzoylecgonine, catalyze the transesterification of cocaine to form cocaethylene and the ethyl esterification of oleic acid to ethyloleate, and is activated by CHAPS. hCE-1 is expressed mostly in liver with lower levels found in the heart and lung.
      References
      Product Information
      FormatSerum
      Control
      • HepG2 cell lysate
      PresentationRabbit polyclonal serum with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Carboxylesterase 1 using this rabbit polyclonal antibody, Anti-hCE-1 Antibody validated for use in western blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected hCE-1 in 10 µg of human liver tissue lysate.
      Western Blotting Analysis: A representative lot from an independent laboratory detected hCE-1 in hCE-1 transfected U373MG cell lysates, and demonstrated a loss of signal in non-transfected and hCE-2 transfected U373MG cell lysates (Prof. P. Potter, St. Jude Children's Research Hospital, Memphis.).
      Biological Information
      ImmunogenLinear peptide corresponding to human hCE-1.
      HostRabbit
      SpecificityThis antibody does not cross react with human CE-2 (Prof. P. Potter, St. Jude Children's Research Hospital, Memphis).
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CES1
      • CES2
      • SES1
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~58 kDa observed. A homodimeric trimer of three hCE-1 molecules may be observed at ~190 kDa in some cell lysates (Prof. P. Potter, St. Jude Children's Research Hospital, Memphis.).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HepG2 cell lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected hCE-1 in 10 µg of HepG2 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      ABS310 04053252909955

      Documentation

      Anti-hCE-1 Antibody (M)SDS

      タイトル

      英語版製品安全データシート((M)SDS) 

      Anti-hCE-1 Antibody 試験成績書(CoA)

      タイトルロット番号
      Anti-hCE-1 - Q2197817 Q2197817

      参考資料

      参考資料の概要Pub Med ID
      Nerve agent hydrolysis activity designed into a human drug metabolism enzyme.
      Hemmert, Andrew C, et al.
      PLoS ONE, 6: e17441 (2011)  2011

      概要を表示する
      21445272 21445272
      An improved human carboxylesterase for enzyme/prodrug therapy with CPT-11.
      Wierdl, M, et al.
      Cancer Gene Ther., 15: 183-92 (2008)  2008

      概要を表示する
      18188187 18188187