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MABC1746-25UG Anti-dimethyl PDCD4 (Arg110) Antibody, clone 3E7

Anti-dimethyl PDCD4 (Arg110), clone 3E7, Cat. No. MABC1746, is a mouse monoclonal antibody that detects Programmed cell death protein 4 and is tested for use in Immunocytochemistry and Western Blotting.

MABC1746-25UG
25 μg  
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      概要

      Replacement Information
      Description
      Catalogue NumberMABC1746-25UG
      DescriptionAnti-dimethyl PDCD4 (Arg110) Antibody, clone 3E7
      Alternate Names
      • Programmed cell death protein 4
      • Neoplastic transformation inhibitor protein
      • Nuclear antigen H731-like
      • Protein 197/15a
      Background InformationProgrammed cell death protein 4 (UniProt: Q53EL6; also known as Neoplastic transformation inhibitor protein, Nuclear antigen H731-like, Protein 197/15a, PDCD4) is encoded by the PDCD4 (also known as H731) gene (Gene ID: 27250) in human. PDCD4 is a tumor-suppressor protein that is shown to be upregulated during apoptosis. It affects transcription, translation, and signal transduction to suppress tumor growth. Its levels are upregulated in proliferative cells and lower levels are reported in lung cancer and colon carcinoma. Its levels are also shown to be down regulated in transitional cell carcinoma. PDCD4 is reported to shuttle between the nucleus and cytoplasm but is predominantly nuclear under normal growth conditions and when phosphorylated at serine 457. It contains two nuclear localization signal sequences (aa 58-64 and 241-250). PDCD4 can undergo phosphorylation at serine 67 by Ribosomal protein S6 kinase beta-1 (RPS6KB1) in response to mitogens, which promotes its proteasomal degradation. PDCD4 can undergo methylation at arginine 110 that enhances its interaction with elF4A. The methylated form of PDCD4 is reported to promote tumor viability during nutrient deprivation, which allows tumors to grow more aggressively. This methylation of arginine 110 can reinforce the interaction between the MA3 domains and eIF4A thereby forming a stronger interaction and may also make PDCD4 MA3 domains more accessible for interaction with eIF4A. (Ref.: Goke, R., et al. (2004). Ann. NY Acad. Sci. 1014; 220-1; Fischer, N., et al. (2012). Biochem. Biophys. Res. Commun. 417(6); 29-34; Fay, MM., et al. (2014). J. Biol. Chem. 289(25); 17541-17552).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-dimethyl PDCD4 (Arg110), clone 3E7, Cat. No. MABC1746, is a mouse monoclonal antibody that detects Programmed cell death protein 4 and is tested for use in Immunocytochemistry and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected dimethyl PDCD4 (Arg110) in Western Blotting applications (Fay, M.M., et. al. (2014). J Biol Chem. 289(25):17541-52).

      Immunocytochemistry Analysis: A representative lot detected dimethyl PDCD4 (Arg110) in Immunocytochemistry applications (Fay, M.M., et. al. (2014). J Biol Chem. 289(25):17541-52).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 18 amino acids from the N-terminal half of human Programmed cell death protein 4 with Arg 110 symmetrically dimethylated.
      EpitopeN-terminal half
      Clone3E7
      Concentration1 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 3E7 is a mouse monoclonal antibody that detects Programmed cell death protein 4 (PDCD4) with symmetrically dimethylated Arg 10. It targets an epitope within 18 amino acids from the N-terminal half.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PDCD4
      • H731
      Modifications
      • Methylation
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight76 kDa observed; 51.73 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293 cells transfected with GFP-PDCD4.

      Western Blotting Analysis: 2 µg/mL of this antibody detected Programmed cell death protein 4 (PDCD4) in HEK293 cells transfected with GFP-PDCD4, but not in cells transfected with GFP-PDCD4+Adenosine dialdehyde (AdOx; methylation inhibited), or with GFP-PDCD4 with R110K mutation.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      MABC1746-25UG 04061842378173

      Documentation

      Anti-dimethyl PDCD4 (Arg110) Antibody, clone 3E7 (M)SDS

      タイトル

      英語版製品安全データシート（(M)SDS） 

      Anti-dimethyl PDCD4 (Arg110) Antibody, clone 3E7 試験成績書（CoA）

      タイトルロット番号
      Anti-dimethyl PDCD4 (Arg110), clone 3E7 - Q3484321 Q3484321

      参考資料

      参考資料の概要Pub Med ID
      Enhanced arginine methylation of programmed cell death 4 protein during nutrient deprivation promotes tumor cell viability.
      Fay MM, Clegg JM, Uchida KA, Powers MA, Ullman KS.
      J Biol Chem  289(25)  17541-52  2014

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