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ABC457 Anti-dUTPase Antibody

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ABC457
100 µg  
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      概要

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      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberABC457
      DescriptionAnti-dUTPase Antibody
      Alternate Names
      • Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial
      • dUTPase
      • dUTP pyrophosphatase
      • dUTPase
      Background InformationdUTPase, also known as dUTP pyrophosphatase, and encoded by the gene DUT, is a critical enzyme involved in nucleotide metabolism for the production of dUMP, the immediate precursor of thymidine nucleotides. dUTPase normally can also function to decrease the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA and cause mutations. dUTPase is localized to the nucleus and mitochondrial locations. dUTPase is expressed in numerous tissues and has multiple isoforms. Some isoforms are constitutively expressed while others match DNA replication cycles. Because dUTPase plays a pivotal role in regulating cellular dUTP pools, this enzyme could have profound effects on the efficacy of agents that target thymidylate biosynthesis and dUTPase expression is being examined as a prognostic marker for overall survival and response to therapy in colon and other cancers where thymidylate therapy is used.
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-dUTPase Antibody is a highly specific rabbit polyclonal antibody, that targets dUTPase & has been tested in western blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: 2 µg/mL from a representative lot detected dUTPase in 10 µg of A431, HEK293, and HeLa cell lysates and human placenta tissue lysate.

      Western Blotting Analysis: A representative lot from an independent laboratory detected dUTPase in MDA-MB231, MCF-7, H184B5F5/M10 cell lysate (Hu, C. M., et al. (2012). Cancer Cell. 22(1):36-50.).
      Biological Information
      ImmunogenRecombinant protein corresponding to human dUTPase.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • DUT
      Purification MethodProtein A Purfied
      UniProt Number
      Molecular Weight~18 kDa observed. The expected molecular weight of this protein is 27 kDa. However, this protein has been observed at 18 kDa (Prof. Z. F. Chang, National Yang-Ming University). Uncharacterized band(s) may be observed in some cell lysates.
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in MDA-MB-231 cell lysate.

      Western Blotting Analysis: 2 µg/mL of this antibody detected dUTPase in 10 µg of MDA-MB-231 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      カタログ番号 GTIN
      ABC457 04053252939549