数量	カタログ番号	注文内容	Qty/Pk	価格
			96-Well Plate

数量	カタログ番号	注文内容	Qty/Pk	価格
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

OP23 Sigma-AldrichAnti-c-H-Ras (Ab-1) Mouse mAb (F235-1.7.1)

This Anti-c-H-Ras (Ab-1) Mouse mAb (F235-1.7.1) is validated for use in Frozen Sections, Immunoblotting, IF, IP, Paraffin Sections for the detection of c-H-Ras (Ab-1).

Anti-c-H-Ras (Ab-1) Mouse mAb (F235-1.7.1): のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。

(M)SDS
試験成績書（CoA）
参考資料
Data Sheet

お勧めの製品

概要

Replacement Information

主要スペック表

Species Reactivity	Host	Antibody Type
H, M, R	M	Monoclonal Antibody

価格＆在庫状況

カタログ番号	在庫状況	包装	Qty/Pk	価格	数量
OP23-100UGCN	在庫状況検索中… — 現在国内在庫なし現在国内在庫有り販売中止在庫僅少現在国内在庫あり在庫を照会するにはログインしてください現在国内在庫有り現在国内在庫なし現在国内在庫有り Remaining : Will advise Remaining : Will advise 注文対象外お問合せください Contact Customer Service お問合せくださいお問合せください	樹脂ｱﾝﾌﾟﾙ	100 μg	価格を検索中… 価格が見つかりません Minimum Quantity needs to be mulitiple of Maximum Quantity is 弊社照会詳細を表示　値引 () — 弊社照会納入価格を閲覧するにはログインしてください	在庫状況を照会 —	お気に入り/ショッピングリストに追加お気に入り/ショッピングリストに追加お気に入りに追加されました
OP23-200UGCN	在庫状況検索中… — 現在国内在庫なし現在国内在庫有り販売中止在庫僅少現在国内在庫あり在庫を照会するにはログインしてください現在国内在庫有り現在国内在庫なし現在国内在庫有り Remaining : Will advise Remaining : Will advise 注文対象外お問合せください Contact Customer Service お問合せくださいお問合せください	樹脂ｱﾝﾌﾟﾙ	200 μg	価格を検索中… 価格が見つかりません Minimum Quantity needs to be mulitiple of Maximum Quantity is 弊社照会詳細を表示　値引 () — 弊社照会納入価格を閲覧するにはログインしてください	在庫状況を照会 —	お気に入り/ショッピングリストに追加お気に入り/ショッピングリストに追加お気に入りに追加されました

Description
Overview	Recognizes the ~21 kDa c-H-Ras protein in normal skin tissue and Rat1 cells. Does not react with c-K-Ras or c-N-Ras p21. A doublet may be seen due to farnesylation.
Catalogue Number	OP23
Brand Family	Calbiochem®

References
References	Rodenhuis, S. et al. 1992. Cancer Res. (Suppl) 52, 2665s. Sidransky, D., et al. 1992. Science 256, 102. Bos, J.L. 1989. Cancer Res. 49, 4682. Furth, M. E., et al. 1987. Oncogene 1, 47. Kraus, M.H., et al. 1984. Proc. Natl. Acad. Sci. USA 81, 5384. Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112. Taparowsky, E., et al. 1983. Cell 34, 581. Ellis, R.W., et al. 1981. Nature 292, 506. Shih, T.Y., et al. 1980. Nature 287, 686.

References

Rodenhuis, S. et al. 1992. Cancer Res. (Suppl) 52, 2665s.
Sidransky, D., et al. 1992. Science 256, 102.
Bos, J.L. 1989. Cancer Res. 49, 4682.
Furth, M. E., et al. 1987. Oncogene 1, 47.
Kraus, M.H., et al. 1984. Proc. Natl. Acad. Sci. USA 81, 5384.
Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112.
Taparowsky, E., et al. 1983. Cell 34, 581.
Ellis, R.W., et al. 1981. Nature 292, 506.
Shih, T.Y., et al. 1980. Nature 287, 686.

Product Information
Form	Liquid
Formulation	In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.4.
Positive control	Normal skin tissue or ras 1 cells
Preservative	≤0.1% sodium azide
Quality Level	MQ100

Applications
Key Applications	Frozen Sections Immunoblotting (Western Blotting) Immunofluorescence Immunoprecipitation Paraffin Sections
Application Notes	Frozen Sections (5 µg/ml) Immunoblotting (10 µg/ml) Immunofluorescence (2.5 µg/ml) Immunoprecipitation (5 µg/ml) Paraffin Sections (5 µg/ml, saponin and pepsin pre-treatment required)
Application Comments	For immunoprecipitation, use 5 µg of Cat. No. OP23 per sample with 45 µl protein G plus agarose. The level of expression of p21Ras is variable in different tissues. For this reason, we recommend a concentration step prior to immunoblotting analysis to obtain optimal results. A doublet may be seen due to farnysylation. Recognizes c-H-Ras and, weakly, v-H-Ras; does not recognize either c-K-Ras or c-N-Ras p21s under the conditions tested. Antibody should be titrated for optimal results in individual systems.

Biological Information
Immunogen	recombinant p21 Ras
Epitope	within amino acids 54-188
Clone	F235-1.7.1
Host	Mouse
Isotype	IgG₁
Species Reactivity	Human Mouse Rat
Antibody Type	Monoclonal Antibody
Concentration Label	Please refer to vial label for lot-specific concentration

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Standard Handling
Storage	+2°C to +8°C
Do not freeze	Yes

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
カタログ番号	GTIN
OP23-100UGCN	04055977208962
OP23-200UGCN	04055977208986

Documentation

Anti-c-H-Ras (Ab-1) Mouse mAb (F235-1.7.1) (M)SDS

タイトル
英語版製品安全データシート（(M)SDS）

Anti-c-H-Ras (Ab-1) Mouse mAb (F235-1.7.1) 試験成績書（CoA）

タイトル	ロット番号
OP23	ロット番号を入力

参考資料

参考資料の概要
Rodenhuis, S. et al. 1992. Cancer Res. (Suppl) 52, 2665s. Sidransky, D., et al. 1992. Science 256, 102. Bos, J.L. 1989. Cancer Res. 49, 4682. Furth, M. E., et al. 1987. Oncogene 1, 47. Kraus, M.H., et al. 1984. Proc. Natl. Acad. Sci. USA 81, 5384. Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112. Taparowsky, E., et al. 1983. Cell 34, 581. Ellis, R.W., et al. 1981. Nature 292, 506. Shih, T.Y., et al. 1980. Nature 287, 686.

参考資料の概要

データシート

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	02-October-2007 RFH
Application	Frozen Sections (5 µg/ml) Immunoblotting (10 µg/ml) Immunofluorescence (2.5 µg/ml) Immunoprecipitation (5 µg/ml) Paraffin Sections (5 µg/ml, saponin and pepsin pre-treatment required)
Description	Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3X63 Ag8.653 mouse myeloma cells. Recognizes the ~21 kDa c-H-Ras protein.
Background	The human ras gene family consists of three identified members, H, K and N-ras, encoding proteins of 188-189 amino acids and 21,000 (p21) molecular weight. Human H- and K-Ras are the homologues of v-H- and v-K-Ras sequences originally isolated from Harvey and Kirsten strains of rat sarcoma viruses. Normal human cellular ras genes can be activated to oncogenes by mutations occurring in codons 12, 13 and 61; such mutated, activated and transforming ras genes have been identified and isolated from human tumors and cultured tumor cells. Although the expression patterns of Ras proteins in normal human tissues are known, similar information for activated ras oncogene encoded p21's and their relevance to human disease diagnosis and prognosis is still emerging.
Host	Mouse
Immunogen	recombinant p21 Ras
Epitope	within amino acids 54-188
Clone	F235-1.7.1
Isotype	IgG₁
Species	human, mouse, rat
Positive control	Normal skin tissue or ras 1 cells
Form	Liquid
Formulation	In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.4.
Concentration Label	Please refer to vial label for lot-specific concentration
Preservative	≤0.1% sodium azide
Comments	For immunoprecipitation, use 5 µg of Cat. No. OP23 per sample with 45 µl protein G plus agarose. The level of expression of p21Ras is variable in different tissues. For this reason, we recommend a concentration step prior to immunoblotting analysis to obtain optimal results. A doublet may be seen due to farnysylation. Recognizes c-H-Ras and, weakly, v-H-Ras; does not recognize either c-K-Ras or c-N-Ras p21s under the conditions tested. Antibody should be titrated for optimal results in individual systems.
Storage	+2°C to +8°C
Do Not Freeze	Yes
Toxicity	Standard Handling
References	Rodenhuis, S. et al. 1992. Cancer Res. (Suppl) 52, 2665s. Sidransky, D., et al. 1992. Science 256, 102. Bos, J.L. 1989. Cancer Res. 49, 4682. Furth, M. E., et al. 1987. Oncogene 1, 47. Kraus, M.H., et al. 1984. Proc. Natl. Acad. Sci. USA 81, 5384. Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112. Taparowsky, E., et al. 1983. Cell 34, 581. Ellis, R.W., et al. 1981. Nature 292, 506. Shih, T.Y., et al. 1980. Nature 287, 686.

カテゴリー

Life Science Research > Antibodies and Assays > Primary Antibodies

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