数量	カタログ番号	注文内容	Qty/Pk	価格
			96-Well Plate

数量	カタログ番号	注文内容	Qty/Pk	価格
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

OP14L Sigma-AldrichAnti-c-ErbB2/c-Neu (Ab-2) Mouse mAb (9G6)

Anti-c-ErbB2/c-Neu (Ab-2), mouse monoclonal, clone 9G6, recognizes the ~185 kDa c-ErbB2/c-Neu SK-BR-3 cells and breast carcinoma. It is validated for IP and IHC on frozen and paraffin sections.

Anti-c-ErbB2/c-Neu (Ab-2) Mouse mAb (9G6): のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。

(M)SDS
試験成績書（CoA）
参考資料
Data Sheet

同義語: Anti-HER2, Anti-Neu, Anti-ErbB2, Anti-Erythroblastosis Virus

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概要

Replacement Information

主要スペック表

Species Reactivity	Host	Antibody Type
H	M	Monoclonal Antibody

価格＆在庫状況

カタログ番号	在庫状況	包装	Qty/Pk	価格	数量
OP14L-100UGCN	在庫状況検索中… — 現在国内在庫なし現在国内在庫有り販売中止在庫僅少現在国内在庫あり在庫を照会するにはログインしてください現在国内在庫有り現在国内在庫なし現在国内在庫有り Remaining : Will advise Remaining : Will advise 注文対象外お問合せください Contact Customer Service お問合せくださいお問合せください	ｶﾞﾗｽﾋﾞﾝ	100 μg	価格を検索中… 価格が見つかりません Minimum Quantity needs to be mulitiple of Maximum Quantity is 弊社照会詳細を表示　値引 () — 弊社照会納入価格を閲覧するにはログインしてください	在庫状況を照会 —	お気に入り/ショッピングリストに追加お気に入り/ショッピングリストに追加お気に入りに追加されました

Description
Overview	Recognizes the ~185 kDa c-ErbB2/c-Neu in SK-BR-3 cells and breast carcinoma tissue. Does not cross-react with v-ErbB2 or the EGF receptor.
Catalogue Number	OP14L
Brand Family	Calbiochem®
Synonyms	Anti-HER2, Anti-Neu, Anti-ErbB2, Anti-Erythroblastosis Virus

References
References	Van De Vijver, M.J., et al.1988 New Engl. J. Med. 319, 1239. DiFiore, P.P., et al.1987 Science 237, 178. Slamon, D.J., et al. 1987 Science 235, 177. Varley, J.M., et al.1987 Oncogene 1, 423. Bargmann, C.I., et al. 1986 Nature 319, 226. Yamamoto, T., et al.1986 Nature 319, 230. Blick, M., et al. 1984 Blood 64, 1234.

Product Information
Form	Lyophilized
Formulation	Lyophilized from a volatile buffer, 100 µg BSA.
Negative control	HepG2 cells or normal skin
Positive control	SK-BR-3 cells or breast carcinoma tissue
Preservative	None
Quality Level	MQ100

Applications
Application References	Paraffin Sections Van De Vijver, M.J., et al. 1988. New Engl. J. Med. 319, 1239.
Key Applications	Frozen Sections Immunoprecipitation Paraffin Sections
Application Notes	Frozen Sections (5 µg/ml) Immunoprecipitation (1 µg/sample) Paraffin Sections (see application references)
Application Comments	Immunoprecipitates the ~185 kDa c-neu protein and has been reported to stain formalin-fixed paraffin-embedded tissue sections of human breast carcinomas overexpressing the c-neu protein. This antibody reacts with a cell surface epitope of c-neu but does not cross-react with the EGF receptor. In one study ductal carcinomas in situ displaying large-cell, comedo growth type, were stained while none of 16 ductal carcinomas in situ of small-cell, papillary, or cribiform growth type were stained by this antibody. Antibody should be titrated for optimal results in individual systems.

Biological Information
Immunogen	cells overexpressing human c-ErbB2/c-Neu
Immunogen	Human
Clone	9G6
Host	Mouse
Isotype	IgG₁
Species Reactivity	Human
Antibody Type	Monoclonal Antibody

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Ambient Temperature Only
Toxicity	Standard Handling
Storage	+2°C to +8°C
Do not freeze	Ok to freeze
Special Instructions	Reconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be reconstituted at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage with 0.1% azide. Avoid freeze/thaw cycles of solutions.

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
カタログ番号	GTIN
OP14L-100UGCN	04055977224825

Documentation

Anti-c-ErbB2/c-Neu (Ab-2) Mouse mAb (9G6) (M)SDS

タイトル
英語版製品安全データシート（(M)SDS）

Anti-c-ErbB2/c-Neu (Ab-2) Mouse mAb (9G6) 試験成績書（CoA）

タイトル	ロット番号
OP14L	ロット番号を入力

参考資料

参考資料の概要
Van De Vijver, M.J., et al.1988 New Engl. J. Med. 319, 1239. DiFiore, P.P., et al.1987 Science 237, 178. Slamon, D.J., et al. 1987 Science 235, 177. Varley, J.M., et al.1987 Oncogene 1, 423. Bargmann, C.I., et al. 1986 Nature 319, 226. Yamamoto, T., et al.1986 Nature 319, 230. Blick, M., et al. 1984 Blood 64, 1234.

データシート

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	02-October-2007 RFH
Synonyms	Anti-HER2, Anti-Neu, Anti-ErbB2, Anti-Erythroblastosis Virus
Application	Frozen Sections (5 µg/ml) Immunoprecipitation (1 µg/sample) Paraffin Sections (see application references)
Description	Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0 Ag14 mouse myeloma cells. Recognizes the ~185 kDa c-ErbB2/c-neu protein.
Background	Amplification of the human proto-oncogene c-neu occurs in approximately 30% of breast carcinomas and the amplification maybe a useful predictor of disease prognosis. Gene amplification and the resulting overexpression of proto-oncogene encoded proteins is thought to transform cells by chronically stimulating signal transduction pathways, and in model systems overexpression of human c-neu induces cellular transformation. In the rat model system, a point mutation has been located within the transmembrane domain of the c-neu oncogene. neu (from neuroglioblastomas) is also referred to as erbB-2 (from erythroblastosis virus) and HER2 (for Human Epidermal growth factor Receptor) and is the second member of the EGF receptor family to be described.
Host	Mouse
Immunogen species	Human
Immunogen	cells overexpressing human c-ErbB2/c-Neu
Clone	9G6
Isotype	IgG₁
Species	human
Positive control	SK-BR-3 cells or breast carcinoma tissue
Negative control	HepG2 cells or normal skin
Form	Lyophilized
Formulation	Lyophilized from a volatile buffer, 100 µg BSA.
Preservative	None
Comments	Immunoprecipitates the ~185 kDa c-neu protein and has been reported to stain formalin-fixed paraffin-embedded tissue sections of human breast carcinomas overexpressing the c-neu protein. This antibody reacts with a cell surface epitope of c-neu but does not cross-react with the EGF receptor. In one study ductal carcinomas in situ displaying large-cell, comedo growth type, were stained while none of 16 ductal carcinomas in situ of small-cell, papillary, or cribiform growth type were stained by this antibody. Antibody should be titrated for optimal results in individual systems.
Storage	+2°C to +8°C
Do Not Freeze	Ok to freeze
Special Instructions	Reconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be reconstituted at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage with 0.1% azide. Avoid freeze/thaw cycles of solutions.
Toxicity	Standard Handling
References	Van De Vijver, M.J., et al.1988 New Engl. J. Med. 319, 1239. DiFiore, P.P., et al.1987 Science 237, 178. Slamon, D.J., et al. 1987 Science 235, 177. Varley, J.M., et al.1987 Oncogene 1, 423. Bargmann, C.I., et al. 1986 Nature 319, 226. Yamamoto, T., et al.1986 Nature 319, 230. Blick, M., et al. 1984 Blood 64, 1234.
Application references	Paraffin Sections Van De Vijver, M.J., et al. 1988. New Engl. J. Med. 319, 1239.

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カタログ番号

説明

OP14

Anti-c-ErbB2/c-Neu (Ab-2) Mouse mAb (9G6)

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カテゴリー

Life Science Research > Antibodies and Assays > Primary Antibodies

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