Millipore Sigma Vibrant Logo

OP14 Anti-c-ErbB2/c-Neu (Ab-2) Mouse mAb (9G6)

Anti-c-ErbB2/c-Neu (Ab-2), mouse monoclonal, clone 9G6, recognizes the ~185 kDa c-ErbB2/c-Neu SK-BR-3 cells and breast carcinoma. It is validated for IP and IHC on frozen and paraffin sections.

Anti-c-ErbB2/c-Neu (Ab-2) Mouse mAb (9G6): のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。

同義語: Anti-ErbB2, Anti-Erythroblastosis Virus, Anti-HER2, Anti-c-Neu

View This Product on Sigma-Aldrich
OP14
価格＆在庫状況を照会

概要

Replacement Information

主要スペック表

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

価格＆在庫状況

カタログ番号 在庫状況包装 Qty/Pk 価格 数量
OP14-100UGCN
在庫状況検索中…
—
現在国内在庫なし 現在国内在庫なし
現在国内在庫有り 
販売中止
在庫僅少
現在国内在庫あり
    Remaining : Will advise
      Remaining : Will advise
      注文対象外
      お問合せください
      Contact Customer Service

      		 樹脂ｱﾝﾌﾟﾙ 100 μg
      価格を検索中…
      価格が見つかりません
      Minimum Quantity needs to be mulitiple of
      Maximum Quantity is
      弊社照会 詳細を表示　
      値引
      ()
       
      —
      弊社照会
      		 —
      Description
      OverviewRecognizes the ~185 kDa c-ErbB2/c-Neu protein in SK-BR-3 cells and breast carcinoma tissue. Does not cross-react with v-ErbB2 or the EGF receptor.
      Catalogue NumberOP14
      Brand Family Calbiochem®
      SynonymsAnti-ErbB2, Anti-Erythroblastosis Virus, Anti-HER2, Anti-c-Neu
      References
      ReferencesVan De Vijver, M.J., et al.1988 New Engl. J. Med. 319, 1239.
      DiFiore, P.P., et al.1987 Science 237, 178.
      Slamon, D.J., et al. 1987 Science 235, 177.
      Varley, J.M., et al.1987 Oncogene 1, 423.
      Bargmann, C.I., et al. 1986 Nature 319, 226.
      Yamamoto, T., et al.1986 Nature 319, 230.
      Blick, M., et al. 1984 Blood 64, 1234.
      Product Information
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
      Negative controlNormal skin tissue or HepG2 cells
      Positive controlSK-BR-3 cells or breast carcinoma tissue
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesParaffin Sections Van De Vijver, M.J., et al. 1988. New Engl. J. Med. 319, 1239.
      Key Applications Frozen Sections
      Immunoprecipitation
      Paraffin Sections
      Application NotesFrozen Sections (5 µg/ml)
      Immunoprecipitation (1 µg/sample)
      Paraffin Sections (see application references)
      Application CommentsImmunoprecipitates the 185 kDa c-neu protein and has been reported to stain formalin-fixed paraffin-embedded tissue sections of human breast carcinomas overexpressing the c-neu protein (see application references). This antibody reacts with a cell surface epitope of c-neu but does not cross-react with the EGF receptor. In one study ductal carcinomas in situ displaying large-cell, comedo growth type, were stained while none of 16 ductal carcinomas in situ of small-cell, papillary, or cribiform growth type were stained by this antibody. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogencells overexpressing human c-ErbB2/c-Neu
      ImmunogenHuman
      Clone9G6
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      OP14-100UGCN 04055977224863

      Documentation

      Anti-c-ErbB2/c-Neu (Ab-2) Mouse mAb (9G6) 試験成績書（CoA）

      タイトルロット番号
      OP14

      参考資料

      参考資料の概要
      Van De Vijver, M.J., et al.1988 New Engl. J. Med. 319, 1239.
      DiFiore, P.P., et al.1987 Science 237, 178.
      Slamon, D.J., et al. 1987 Science 235, 177.
      Varley, J.M., et al.1987 Oncogene 1, 423.
      Bargmann, C.I., et al. 1986 Nature 319, 226.
      Yamamoto, T., et al.1986 Nature 319, 230.
      Blick, M., et al. 1984 Blood 64, 1234.
      データシート

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision29-June-2007 RFH
      SynonymsAnti-ErbB2, Anti-Erythroblastosis Virus, Anti-HER2, Anti-c-Neu
      ApplicationFrozen Sections (5 µg/ml)
      Immunoprecipitation (1 µg/sample)
      Paraffin Sections (see application references)
      DescriptionThis clone expresses the kappa light chain isotype. Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0 Ag14 myeloma cells. Recognizes the ~185 kDa c-ErbB2/c-neu protein.
      BackgroundAmplification of the human proto-oncogene c-neu occurs in approximately 30% of breast carcinomas and the amplification maybe a useful predictor of disease prognosis. Gene amplification and the resulting overexpression of proto-oncogene encoded proteins is thought to transform cells by chronically stimulating signal transduction pathways, and in model systems overexpression of human c-neu induces cellular transformation. In the rat model system, a point mutation has been located within the transmembrane domain of the c-neu oncogene. neu (from neuroglioblastomas) is also referred to as erbB-2 (from erythroblastosis virus) and HER2 (for Human Epidermal growth factor Receptor) and is the second member of the EGF receptor family to be described.
      HostMouse
      Immunogen speciesHuman
      Immunogencells overexpressing human c-ErbB2/c-Neu
      Clone9G6
      IsotypeIgG₁
      Specieshuman
      Positive controlSK-BR-3 cells or breast carcinoma tissue
      Negative controlNormal skin tissue or HepG2 cells
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsImmunoprecipitates the 185 kDa c-neu protein and has been reported to stain formalin-fixed paraffin-embedded tissue sections of human breast carcinomas overexpressing the c-neu protein (see application references). This antibody reacts with a cell surface epitope of c-neu but does not cross-react with the EGF receptor. In one study ductal carcinomas in situ displaying large-cell, comedo growth type, were stained while none of 16 ductal carcinomas in situ of small-cell, papillary, or cribiform growth type were stained by this antibody. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesVan De Vijver, M.J., et al.1988 New Engl. J. Med. 319, 1239.
      DiFiore, P.P., et al.1987 Science 237, 178.
      Slamon, D.J., et al. 1987 Science 235, 177.
      Varley, J.M., et al.1987 Oncogene 1, 423.
      Bargmann, C.I., et al. 1986 Nature 319, 226.
      Yamamoto, T., et al.1986 Nature 319, 230.
      Blick, M., et al. 1984 Blood 64, 1234.
      Application referencesParaffin Sections Van De Vijver, M.J., et al. 1988. New Engl. J. Med. 319, 1239.

      関連製品＆アプリケーション

      Related Products

      カタログ番号 説明  
      OP14L Anti-c-ErbB2/c-Neu (Ab-2) Mouse mAb (9G6) 価格＆在庫状況を表示

      カテゴリー

      Life Science Research > Antibodies and Assays > Primary Antibodies