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ABE471 Anti-WAC Antibody, CT

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ABE471
100 µg  
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      概要

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      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IP, ChIPRbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE471
      DescriptionAnti-WAC Antibody, CT
      Alternate Names
      • WW domain-containing adapter protein with coiled-coil
      • WAC
      Background InformationWAC (WW domain-containing adapter protein with coiled-coil) functions as a linker between gene transcription and histone H2B monoubiquitination at 'Lys-120' (H2BK120ub1). Wac interacts with the RNA polymerase II transcriptional machinery through its WW domain and with RNF20-RNF40 via its coiled-coil region. It has been shown to regulate H2BK120ub1, gene transcription, cell-cycle checkpoint activation in response to DNA damage, positively regulate amino acid starvation-induced autophagy and negatively regulate the ubiquitin proteasome pathway. In nuclear speckles it has been observed to colocalize with pre-mRNA processing complexes. This antibody recognizes the C-terminus of the WAC protein.
      Product Information
      FormatPurified
      Control
      • K562 cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-WAC, C-terminus detects levels of WAC proteins & has been published & validated for use in wb, IP & ChIP.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesWestern Blotting Analysis: 1 µg/mL of this antibody from a representative lot detected WAC, C-terminus in 10 µg of HEK293 cell lysate.

      Immunoprecipitation Analysis: A representative lot from an independent laboratory detected WAC, N-terminus in 293T cell lysates stably transfected with SFB-RNF20 and in HCT116 cell lysates transfected with siRNA negative control (Zhang, F. and Yu, X. (2011). Mol Cell. 41(4):384-397.).

      Chromatin Immunoprecipitation: A representative lot from an independent laboratory detected WAC, N-terminus in HCT116 cell lysates transfected with siRNA negative control, but demonstrated a loss of signal in HCT166 cell lysates transfected with siRNF20 (Zhang, F. and Yu, X. (2011). Mol Cell. 41(4):384-397.).

      Westerm Blotting Analysis: A representative lot from an independent laboratory detected WAC in 293T cell lysates transfected with siRNA negative control, but demonstrated a loss of signal in 293T cell lysates transfected with siWAC (Zhang, F. and Yu, X. (2011). Mol Cell. 41(4):384-397.).
      Biological Information
      ImmunogenRecombinant protein corresponding to human WAC.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • WAC
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~80 kDa observed. An uncharacterized band may be observed at ~95 kDa and ~120 kDa in some cell lysates.
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in K562 cell lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected WAC, C-terminus in 10 µg of K562 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      カタログ番号 GTIN
      ABE471 04053252858215