数量	カタログ番号	注文内容	Qty/Pk	価格
			96-Well Plate

数量	カタログ番号	注文内容	Qty/Pk	価格
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

DR1047 Sigma-AldrichAnti-UHRF1 Mouse mAb (3A11)

This Anti-UHRF1 Mouse mAb (3A11) is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of UHRF1.

Anti-UHRF1 Mouse mAb (3A11): のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。

(M)SDS
参考資料
Data Sheet

同義語: Anti-Ubiquitin-Like PHD/RING Finger Domain 1

お勧めの製品

概要

Replacement Information

主要スペック表

Species Reactivity	Host	Antibody Type
H	M	Monoclonal Antibody

価格＆在庫状況

カタログ番号	在庫状況	包装	Qty/Pk	価格	数量
DR1047-100UGCN	在庫状況検索中… — 現在国内在庫なし現在国内在庫有り販売中止在庫僅少現在国内在庫あり在庫を照会するにはログインしてください現在国内在庫有り現在国内在庫なし現在国内在庫有り Remaining : Will advise Remaining : Will advise 注文対象外お問合せください Contact Customer Service お問合せくださいお問合せください		100 μg	価格を検索中… 価格が見つかりません Minimum Quantity needs to be mulitiple of Maximum Quantity is 弊社照会詳細を表示　値引 () — 弊社照会納入価格を閲覧するにはログインしてください	在庫状況を照会 —	お気に入り/ショッピングリストに追加お気に入り/ショッピングリストに追加お気に入りに追加されました

Description
Overview	Recognizes the ~90 kDa UHRF1 protein in HeLa cells and human endometrium tissue.
Catalogue Number	DR1047
Brand Family	Calbiochem®
Synonyms	Anti-Ubiquitin-Like PHD/RING Finger Domain 1
Application Data	Detection of human UHRF1 by immunoblotting. Sample: Whole cell lysate from HeLa NE cells. Primary antibody: Anti-UHRF1 Mouse mAb (3A11) (Cat. No. DR1047) (1-5 µg/ml). Detection: chemiluminescence. Detection of human UHRF1 by staining paraffin sections. Sample: Formalin-fixed, paraffin embedded human endometrium tissue. Primary antibody: Anti-UHRF1 Mouse mAb (3A11) (Cat. No. DR1047) (3 µg/ml). Detection: colorimetric (DAB). Detection of human UHRF1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-UHRF1 Mouse mAb (3A11) (Cat. No. DR1047) (10 µg/ml). Detection: fluorescence.

References
References	Mistry H, et al. 2008. Biochem. Biophys. Res. Commun. 375 321.

Product Information
Form	liquid
Formulation	In PBS, pH 7.2.
Positive control	HeLa cells, Human endometrium tissue
Preservative	None
Quality Level	MQ100

Applications
Key Applications	Enzyme-Linked Immunosorbent Assay Immunoblotting (Western Blotting) Immunocytochemistry Paraffin Sections
Application Notes	ELISA (see comments) Immunoblotting (1-5 µg/ml) Immunocytochemistry (10 µg/ml) Paraffin Sections (3 µg/ml)
Application Comments	For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Biological Information
Immunogen	A recombinant polypeptide corresponding to amino acides 694-794 of human rUHRF1, expressed as a GST fusion protein
Immunogen	NA
Clone	3A11
Host	Mouse
Isotype	IgG₁
Species Reactivity	Human
Antibody Type	Monoclonal Antibody

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Regulatory Review
Storage	-20°C
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
カタログ番号	GTIN
DR1047-100UGCN	04055977225969

Documentation

Anti-UHRF1 Mouse mAb (3A11) (M)SDS

タイトル
英語版製品安全データシート（(M)SDS）

参考資料

参考資料の概要
Mistry H, et al. 2008. Biochem. Biophys. Res. Commun. 375 321.

データシート

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	01-November-2010 RFH
Synonyms	Anti-Ubiquitin-Like PHD/RING Finger Domain 1
Application	ELISA (see comments) Immunoblotting (1-5 µg/ml) Immunocytochemistry (10 µg/ml) Paraffin Sections (3 µg/ml)
Application Data	Detection of human UHRF1 by immunoblotting. Sample: Whole cell lysate from HeLa NE cells. Primary antibody: Anti-UHRF1 Mouse mAb (3A11) (Cat. No. DR1047) (1-5 µg/ml). Detection: chemiluminescence. Detection of human UHRF1 by staining paraffin sections. Sample: Formalin-fixed, paraffin embedded human endometrium tissue. Primary antibody: Anti-UHRF1 Mouse mAb (3A11) (Cat. No. DR1047) (3 µg/ml). Detection: colorimetric (DAB). Detection of human UHRF1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-UHRF1 Mouse mAb (3A11) (Cat. No. DR1047) (10 µg/ml). Detection: fluorescence.
Description	Purified mouse monoclonal antibody. Recognizes the ~90 kDa UHRF1 protein.
Background	This protein is a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIα and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint
Host	Mouse
Immunogen species	NA
Immunogen	A recombinant polypeptide corresponding to amino acides 694-794 of human rUHRF1, expressed as a GST fusion protein
Clone	3A11
Isotype	IgG₁
Species	human
Positive control	HeLa cells, Human endometrium tissue
Form	liquid
Formulation	In PBS, pH 7.2.
Preservative	None
Comments	For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage	Avoid freeze/thaw -20°C
Do Not Freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).
Toxicity	Regulatory Review
References	Mistry H, et al. 2008. Biochem. Biophys. Res. Commun. 375 321.

カテゴリー

Life Science Research > Antibodies and Assays > Primary Antibodies

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