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MABN814 Anti-Twinkle Antibody, clone 13A10.1

This Anti-Twinkle Antibody, clone 13A10.1 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of Twinkle.

Anti-Twinkle Antibody, clone 13A10.1 : のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。
Research Category: Neuroscience Research Sub-Category: Developmental Signaling Gene Symbol: EPO1, C10orf2 UniProt Number: Q96RR1
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MABN814
100 μg  
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      概要

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      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN814
      DescriptionAnti-Twinkle Antibody, clone 13A10.1
      Alternate Names
      • Twinkle protein, mitochondrial
      • Progressive external ophthalmoplegia 1 protein
      • T7 gp4-like protein with intramitochondrial nucleoid localization
      • T7-like mitochondrial DNA helicase
      • Twinkle
      Background InformationTwinkle protein, mitochondrial (EC 3.6.4.12; UniProt Q96RR1; also known as Ataxin 8, Mitochondrial twinkle protein, Progressive external ophthalmoplegia 1 protein, T7 gp4-like protein with intramitochondrial nucleoid localization, T7 helicase-related protein with intramitochondrial nucleoid localization, T7-like mitochondrial DNA helicase) is encoded by the EPO1 (also known as ATXN8, C10orf2, IOSCA, MTDPS7, PEO, PEO1, PEOA3, PRLTS5, SANDO, SCA8, TWINL) gene (Gene ID 56662) in human. Mitochondrial DNA (mtDNA) replication involves the catalytic and accessory subunits of DNA polymerase gamma, the mtDNA helicase Twinkle, the mitochondrial single-stranded DNA-binding protein, and the mitochondrial RNA polymerase. Cells expressing Twinkle mutants accumulate double-stranded mtDNA replication intermediates with loss of RNA associated with the lagging strand, consistent with the role of Twinkle in unwinding double-stranded mtDNA. Mutations in the human Twinkle gene (C10orf2) cause autosomal dominant progressive external ophthalmoplegia (PEO), associated with multiple mtDNA deletions.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Twinkle Antibody, clone 13A10.1 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of Twinkle.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesWestern Blotting Analysis: 0.5 µg/mL from a representative lot detected Twinkle in 10 µg of K562 cell lysate.
      Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Twinkle in human cerebral cortex and human skeletal muscle tissue.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to the C-terminal of human Twinkle.
      EpitopeC-terminal
      Clone13A10.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • EPO1
      • C10orf2
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~77 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HL-60 cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected Twinkle in 10 µg of HL-60 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      MABN814 04055977274967

      Documentation

      Anti-Twinkle Antibody, clone 13A10.1 (M)SDS

      タイトル

      英語版製品安全データシート（(M)SDS） 

      Anti-Twinkle Antibody, clone 13A10.1 試験成績書（CoA）

      タイトルロット番号
      Anti-Twinkle, clone 13A10.1 - 3446267 3446267
      Anti-Twinkle, clone 13A10.1 - 3873554 3873554
      Anti-Twinkle, clone 13A10.1 - 3992934 3992934
      Anti-Twinkle, clone 13A10.1 -Q2592793 Q2592793

      技術情報

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      White Paper: Further considerations of antibody validation and usage.