数量	カタログ番号	注文内容	Qty/Pk	価格
			96-Well Plate

数量	カタログ番号	注文内容	Qty/Pk	価格
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

CP06 Sigma-AldrichAnti-α-Tubulin Mouse mAb (DM1A)

This Anti-α-Tubulin Mouse mAb (DM1A) is validated for use in Immunoblotting, Immunofluorescence for the detection of α-Tubulin.

Anti-α-Tubulin Mouse mAb (DM1A): のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。

(M)SDS
試験成績書（CoA）
参考資料
Data Sheet
Citations

お勧めの製品

概要

Replacement Information

主要スペック表

Species Reactivity	Host	Antibody Type
Ch, Gr, H, M, R	M	Monoclonal Antibody

価格＆在庫状況

カタログ番号	在庫状況	包装	Qty/Pk	価格	数量
CP06-100UGCN	在庫状況検索中… — 現在国内在庫なし現在国内在庫有り販売中止在庫僅少現在国内在庫あり在庫を照会するにはログインしてください現在国内在庫有り現在国内在庫なし現在国内在庫有り Remaining : Will advise Remaining : Will advise 注文対象外お問合せください Contact Customer Service お問合せくださいお問合せください	樹脂ｱﾝﾌﾟﾙ	100 μg	価格を検索中… 価格が見つかりません Minimum Quantity needs to be mulitiple of Maximum Quantity is 弊社照会詳細を表示　値引 () — 弊社照会納入価格を閲覧するにはログインしてください	在庫状況を照会 —	お気に入り/ショッピングリストに追加お気に入り/ショッピングリストに追加お気に入りに追加されました

Description
Overview	Recognizes the ~60 kDa tubulin protein found in all eukaryotic cells. May be used as a positive control.
Catalogue Number	CP06
Brand Family	Calbiochem®

References
References	Blose, S.H., et al. 1984. J. Cell. Biol. 98, 847. Lin, J.J.C., et al. 1984. In: Monoclonal antibodies and functional cell lines. Kennett, R.H., Bechtol, K.B. and McKearn, T.J., eds. Plenum Publishing Corp. Lin, J.J.C. 1982. Cold Spring Harbor Symp. on Quant. Biol. 46, 769. Goldman, R.D., et al. 1979. Ann. Rev. Physiol. 41, 701. Goldman, R., Pollard, T. and Rosenbaum, J. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C, Cold Spring Harbor, New York.

References

Blose, S.H., et al. 1984. J. Cell. Biol. 98, 847.
Lin, J.J.C., et al. 1984. In: Monoclonal antibodies and functional cell lines. Kennett, R.H., Bechtol, K.B. and McKearn, T.J., eds. Plenum Publishing Corp.
Lin, J.J.C. 1982. Cold Spring Harbor Symp. on Quant. Biol. 46, 769.
Goldman, R.D., et al. 1979. Ann. Rev. Physiol. 41, 701.
Goldman, R., Pollard, T. and Rosenbaum, J. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C, Cold Spring Harbor, New York.

Product Information
Form	Liquid
Formulation	In 50 mM sodium phosphate buffer, 0.2% gelatin.
Negative control	trp E (Ab-1)
Positive control	Any cell line
Preservative	≤0.1% sodium azide
Quality Level	MQ100

Applications
Application References	Immunoblotting Gagarin, D., et al. 2005. J. Mol. Cell. Card. 39, 453. Yang, Z., et al. 2007. J. Vasc. Res. 44, 483.
Key Applications	Immunoblotting (Western Blotting) Immunofluorescence
Application Notes	Immunoblotting (20-100 ng/ml, colorimetric or chemiluminescence; see application references) Immunofluorescence (5 µg/ml)
Application Comments	Tubulin is found in all cells and is used as a positive control for immunofluorescence or immunoblotting. Antibody should be titrated for optimal results in individual systems.

Biological Information
Immunogen	native chick brain microtubules
Immunogen	Chicken
Clone	DM1A
Host	Mouse
Isotype	IgG₁
Species Reactivity	Chicken Gerbil Human Mouse Rat
Antibody Type	Monoclonal Antibody
Concentration Label	Please refer to vial label for lot-specific concentration

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Standard Handling
Storage	+2°C to +8°C
Do not freeze	Yes

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
カタログ番号	GTIN
CP06-100UGCN	04055977220933

Documentation

Anti-α-Tubulin Mouse mAb (DM1A) (M)SDS

タイトル
英語版製品安全データシート（(M)SDS）

Anti-α-Tubulin Mouse mAb (DM1A) 試験成績書（CoA）

タイトル	ロット番号
CP06	ロット番号を入力

参考資料

参考資料の概要
Blose, S.H., et al. 1984. J. Cell. Biol. 98, 847. Lin, J.J.C., et al. 1984. In: Monoclonal antibodies and functional cell lines. Kennett, R.H., Bechtol, K.B. and McKearn, T.J., eds. Plenum Publishing Corp. Lin, J.J.C. 1982. Cold Spring Harbor Symp. on Quant. Biol. 46, 769. Goldman, R.D., et al. 1979. Ann. Rev. Physiol. 41, 701. Goldman, R., Pollard, T. and Rosenbaum, J. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C, Cold Spring Harbor, New York.

参考資料の概要

引用

Title

Saumen Pal, et al. (2006) An antiangiogenic neurokinin-B/thromboxane A2 regulatory axis. Journal of Cell Biology 174, 1047-1058.

Zhonghua Gao, Yufang Shao and Xuejun Jiang. (2005) Essential roles of the Bcl-2 family of proteins in caspase-2-induced apoptosis. Journal of Biological Chemistry 280, 38271-38275.

Annie Huang, et al. (2005) Identification of a novel c-myc protein interactor, JPO2, with transforming activity in medulloblastoma cells. Cancer Research 65, 5607-5619.

Thomas Simmen, et al. (2005) PACS-2 controls endoplasmic reticulum-mitochondria communication and Bid-mediated apoptosis. European Molecular Biology Organization Journal 24, 717-729.

データシート

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	30-November-2007 JSW
Application	Immunoblotting (20-100 ng/ml, colorimetric or chemiluminescence; see application references) Immunofluorescence (5 µg/ml)
Description	Purified mouse monoclonal antibody generated by immunizing Balb/c57 mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Recognizes the ~60 kDa tubulin protein.
Background	Cytoskeletal proteins are found in highly organized arrays within the cytoplasm of higher eukaryotic cells. These proteins are intimately involved in functions such as cell and intracellular organelle movement, maintenance of cell shape, and endocytosis. The cytoskeleton of most eukaryotic cells is comprised of at least three fibrous systems each with accessory proteins: the microfilaments, composed of actin; the microfibrils, composed of tubulin; and the intermediate filaments composed of vimentin. Monoclonal antibodies have been used to study the components of the cytoskeleton and their intracellular location, changes in the expression of these elements during differentiation, and the function of the cytoskeleton in living cells by microinjection of the antibodies.
Host	Mouse
Immunogen species	Chicken
Immunogen	native chick brain microtubules
Clone	DM1A
Isotype	IgG₁
Species	chicken, gerbil, human, mouse, rat
Positive control	Any cell line
Negative control	trp E (Ab-1)
Form	Liquid
Formulation	In 50 mM sodium phosphate buffer, 0.2% gelatin.
Concentration Label	Please refer to vial label for lot-specific concentration
Preservative	≤0.1% sodium azide
Comments	Tubulin is found in all cells and is used as a positive control for immunofluorescence or immunoblotting. Antibody should be titrated for optimal results in individual systems.
Storage	+2°C to +8°C
Do Not Freeze	Yes
Toxicity	Standard Handling
References	Blose, S.H., et al. 1984. J. Cell. Biol. 98, 847. Lin, J.J.C., et al. 1984. In: Monoclonal antibodies and functional cell lines. Kennett, R.H., Bechtol, K.B. and McKearn, T.J., eds. Plenum Publishing Corp. Lin, J.J.C. 1982. Cold Spring Harbor Symp. on Quant. Biol. 46, 769. Goldman, R.D., et al. 1979. Ann. Rev. Physiol. 41, 701. Goldman, R., Pollard, T. and Rosenbaum, J. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C, Cold Spring Harbor, New York.
Citation	Saumen Pal, et al. (2006) An antiangiogenic neurokinin-B/thromboxane A2 regulatory axis. Journal of Cell Biology 174, 1047-1058. Zhonghua Gao, Yufang Shao and Xuejun Jiang. (2005) Essential roles of the Bcl-2 family of proteins in caspase-2-induced apoptosis. Journal of Biological Chemistry 280, 38271-38275. Annie Huang, et al. (2005) Identification of a novel c-myc protein interactor, JPO2, with transforming activity in medulloblastoma cells. Cancer Research 65, 5607-5619. Thomas Simmen, et al. (2005) PACS-2 controls endoplasmic reticulum-mitochondria communication and Bid-mediated apoptosis. European Molecular Biology Organization Journal 24, 717-729.
Application references	Immunoblotting Gagarin, D., et al. 2005. J. Mol. Cell. Card. 39, 453. Yang, Z., et al. 2007. J. Vasc. Res. 44, 483.

カテゴリー

Life Science Research > Antibodies and Assays > Primary Antibodies

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