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MABT1338-100UL Anti-Tropomyosin alpha-1 Antibody, clone 3F5E4

This mouse monoclonal Anti-Tropomyosin alpha-1, clone 3F5E4, Cat. No. MABT1338 is tested for use in Western Blotting, for the detection of Tropomyosin alpha-1 chain.

MABT1338-100UL
100 µL  
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      概要

      Replacement Information
      Description
      Catalogue NumberMABT1338-100UL
      DescriptionAnti-Tropomyosin alpha-1 Antibody, clone 3F5E4
      Alternate Names
      • Tropomyosin alpha-1 chain
      • Alpha-tropomyosin
      • Tropomyosin-1
      Background InformationTropomyosin alpha-1 chain (UniProt: P04692; also known as Alpha-tropomyosin, Tropomyosin-1) is encoded by the Tpm1 (also known as Alpha-tm, Tpma) gene (Gene ID: 24851) in rat. Tropomyosins are dimers of coiled-coil proteins that provide stability to actin filaments and regulate access of other actin-binding proteins. Tropomyosin-1 is a cytoskeletal protein that associates with F-actin stress fibers. It binds to actin filaments in muscle and non-muscle cells and plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. It stabilizes actin filaments and regulates the interaction of actin filament with other actin-binding proteins. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. Tropomyosin-1 is a homodimeric protein, but can form heterodimer of an alpha (TPM1, TPM3, or TPM4) and a beta (TPM2) chain. Mutations in TPM1 gene have been linked to cardiomyopathy characterized by ventricular hypertrophy and ventricular dilation and impaired systolic function that results in congestive heart failure and arrhythmia. Eight different isoforms of Tropomyosin-1 have been described in rat that are produced by alternative splicing. (Ref.: Schevzov, G., et al. (2011). BioArchitecture, 1 (4); 135-164).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationThis mouse monoclonal Anti-Tropomyosin alpha-1, clone 3F5E4, Cat. No. MABT1338 is tested for use in Western Blotting, for the detection of Tropomyosin alpha-1 chain.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected Tropomyosin alpha-1 in Western Blotting applications (Schevzov, G., et. al. (2011). Bioarchitecture. 1(4):135-164).
      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 24 amino acids from the N-terminal region of rat Tropomyosin alpha-1 chain, isoform 2.
      Clone3F5E4
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 3F5E4 is a mouse monoclonal antibody that detects Tropomyosin alpha-1 chain. It targets an epitope within 24 amino acids from the N-terminal half corresponding to exon2a region in TPM1 gene.
      IsotypeIgG1κ
      Species Reactivity
      • Rat
      • Mouse
      Species Reactivity NoteMouse, Rat.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • TPM1
      • C15orf13
      • TMSA
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~34 kDa observed; 32.68 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse brain tissue lysates (Neg Ctrl) and mouse stomach tissue lysates (Pos Ctrl).

      Western Blotting Analysis: A 1:250 dilution of this antibody detected Tropomyosin alpha-1 in mouse brain tissue lysates (Neg Ctrl) and mouse stomach tissue lysates (Pos Ctrl).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      MABT1338-100UL 04061841844433

      Documentation

      Anti-Tropomyosin alpha-1 Antibody, clone 3F5E4 (M)SDS

      タイトル

      英語版製品安全データシート（(M)SDS） 

      Anti-Tropomyosin alpha-1 Antibody, clone 3F5E4 試験成績書（CoA）

      タイトルロット番号
      Anti-Tropomyosin alpha-1, clone 3F5E4 - Q3437398 Q3437398

      参考資料

      参考資料の概要Pub Med ID
      Tropomyosin isoforms and reagents
      Galina Schevzov 1 , Shane P Whittaker, Thomas Fath, Jim Jc Lin, Peter W Gunning
      Bioarchitecture  1(4)  135-164  2011

      概要を表示する       		22069507 22069507