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MABC1717-25UL Anti-Tn mIgM (STn-PS A1), clone KT-IgM-8

Anti-Tn mIgM, clone KT-IgM-8, Cat. No. MABC1717, is a mouse monoclonal antibody that detects Tn antigen and is tested in ELISA, Flow Cytometry, Complement Dependent Cytotoxicity, Antibody Dependent Cellular Cytotoxicity, Glycan Array analysis, SPR, ITC and in vivo tumor challenge experiments.

MABC1717-25UL
25 µL  
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      概要

      Replacement Information
      Description
      Catalogue NumberMABC1717-25UL
      DescriptionAnti-Tn mIgM (STn-PS A1), clone KT-IgM-8
      Alternate Names
      • Thomsen-nouveau (Tn)
      • Tn antigen
      • Tn saccharide
      • N-acetyl Galactosamine
      • Alpha-GalNAc
      • Alpha GalpNAc
      • GalNAc
      • GalNAc-alpha
      • N-acetyl-alpha-D-galactosamine
      • Blood group A antigen
      Background InformationThe Thomsen-nouveau (Tn) monosaccharide is a tumor-associated carbohydrate antigen (TACA) that has been shown to be overexpressed in several carcinomas, including, but not limited to, breast, ovarian, and colon cancers. It is not detected in normal non-cancerous tissues. The Tn antigen (GalNAc) is an O-linked mucin TACA. Mucins are highly glycosylated proteins in epithelial cells. Transmembrane mucins are known to contribute to transmit growth and survival signals to the interior of cells. In many epithelial surfaces, mucins shield and protect cells from infections by pathogens. The Tn antigen arises from a blockage in the normal O-glycosylation pathway in which glycans are extended from the common precursor GalNAcα1-O-Ser/Thr. The precursor is generated in the Golgi apparatus on newly synthesized glycoproteins by a family of polypeptide α-N-acetylgalactosaminyltransferases (ppGalNAcTs) and then extended to the common core 1 O-glycan Galβ1-3GalNAcα1-O-Ser/Thr (T antigen) by a single enzyme termed the T-synthase (core 1 β3-galactosyltransferase or C1GalT). (Ref.: De Silva, R. A.; et al. (2009). J. Am. Chem. Soc. 131(28); 9622-9623).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgM in PBS without azide.
      Applications
      ApplicationAnti-Tn mIgM, clone KT-IgM-8, Cat. No. MABC1717, is a mouse monoclonal antibody that detects Tn antigen and is tested in ELISA, Flow Cytometry, Complement Dependent Cytotoxicity, Antibody Dependent Cellular Cytotoxicity, Glycan Array analysis, SPR, ITC and in vivo tumor challenge experiments.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Application NotesAffects Function Analysis: A representative lot showed high complement-dependent cytotoxicity (CDC) activity. (Trabbic, K.R., et. al. (2018). Cancer Immunol Immunother. 67(9):1437-1447).

      Flow Cytometry Analysis: A representative lot detected Tn-antigen in Flow Cytometry applications (Trabbic, K.R., et. al. (2018). Cancer Immunol Immunother. 67(9):1437-1447).

      Enzyme Immunoassay Analysis: A representative lot detected Tn-antigen in ELISA applications (Trabbic, K.R., et. al. (2018). Cancer Immunol Immunother. 67(9):1437-1447).
      Biological Information
      ImmunogenSemi-synthetic Tn-PS A1 (Tn= D-GalpNAc)
      CloneKt-IgM-8
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone KT-IgM-8 is a mouse monoclonal antibody that detects Thomsen-nouveau (Tn) polysaccharide A1 monovalently, including in human tumor cells.
      IsotypeIgM
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Purification MethodLigatrap
      UniProt Number
      Molecular WeightN/A
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in MCF-7 cells.

      Flow Cytometry Analysis: 1 µg of this antibody detected Tn-antigen in one million MCF-7 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      MABC1717-25UL 04054839029004

      Documentation

      Anti-Tn mIgM (STn-PS A1), clone KT-IgM-8 (M)SDS

      タイトル

      英語版製品安全データシート（(M)SDS） 

      Anti-Tn mIgM (STn-PS A1), clone KT-IgM-8 試験成績書（CoA）

      タイトルロット番号
      Anti-STn-PS A1, clone Kt-IgM-8 - 3277859 3277859

      参考資料

      参考資料の概要Pub Med ID
      Production of a mouse monoclonal IgM antibody that targets the carbohydrate Thomsen-nouveau cancer antigen resulting in in vivo and in vitro tumor killing.
      Trabbic, KR; Kleski, KA; Shi, M; Bourgault, JP; Prendergast, JM; Dransfield, DT; Andreana, PR
      Cancer Immunol Immunother  67  1437-1447  2018

      概要を表示する       		30030557 30030557