数量	カタログ番号	注文内容	Qty/Pk	価格
			96-Well Plate

数量	カタログ番号	注文内容	Qty/Pk	価格
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

610205 Sigma-AldrichAnti-Tie2 Mouse mAb (Ab33)

This Anti-Tie2 Mouse mAb (Ab33) is validated for use in Frozen Sections, Immunoblotting, Immunoprecipitation, Paraffin Sections for the detection of Tie2.

Anti-Tie2 Mouse mAb (Ab33): のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。

(M)SDS
試験成績書（CoA）
参考資料
Data Sheet

同義語: Anti-Angiopoietin Receptor, Anti-Tek

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概要

Replacement Information

主要スペック表

Species Reactivity	Host	Antibody Type
H, M, Porcine, R	M	Monoclonal Antibody

価格＆在庫状況

カタログ番号	在庫状況	包装	Qty/Pk	価格	数量
610205-50UGCN	在庫状況検索中… — 現在国内在庫なし現在国内在庫有り販売中止在庫僅少現在国内在庫あり在庫を照会するにはログインしてください現在国内在庫有り現在国内在庫なし現在国内在庫有り Remaining : Will advise Remaining : Will advise 注文対象外お問合せください Contact Customer Service お問合せくださいお問合せください	樹脂ｱﾝﾌﾟﾙ	50 μg	価格を検索中… 価格が見つかりません Minimum Quantity needs to be mulitiple of Maximum Quantity is 弊社照会詳細を表示　値引 () — 弊社照会納入価格を閲覧するにはログインしてください	在庫状況を照会 —	お気に入り/ショッピングリストに追加お気に入り/ショッピングリストに追加お気に入りに追加されました

Description
Overview	Recognizes the ~140-45 kDa Tie2 protein.
Catalogue Number	610205
Brand Family	Calbiochem®
Synonyms	Anti-Angiopoietin Receptor, Anti-Tek

References
References	Lin, P., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 8829. Wong, A.L., et al. 1997. Circ. Res. 81, 567. Peters, K.G., et al. 1998. Br. J. Cancer 77, 51.

Product Information
Form	Liquid
Formulation	In 150 mM NaCl, 100 mM Tris-glycine, 30% glycerol, pH 7.4.
Preservative	≤0.1% sodium azide
Quality Level	MQ100

Applications
Key Applications	Frozen Sections Immunoblotting (Western Blotting) Immunoprecipitation Not Paraffin Sections
Application Notes	Frozen Sections (see comments) Immunoblotting (0.2-1 µg/ml, chemiluminescence) Immunoprecipitation (see comments) Paraffin Sections (not recommended)
Application Comments	For frozen sections, tissues were treated with Triton^®-X-100 detergent. This antibody has also been reported to work for immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.

Biological Information
Immunogen	a recombinant protein consisting of amino acids 1-745 of human Tie2 fused to a His•Tag^® sequence
Immunogen	Human
Clone	Ab33
Host	Mouse
Isotype	IgG₁
Species Reactivity	Human Mouse Porcine Rat
Antibody Type	Monoclonal Antibody
Concentration Label	Please refer to vial label for lot-specific concentration

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Dry Ice Only
Toxicity	Standard Handling
Storage	-20°C
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
カタログ番号	GTIN
610205-50UGCN	04055977264197

Documentation

Anti-Tie2 Mouse mAb (Ab33) (M)SDS

タイトル
英語版製品安全データシート（(M)SDS）

Anti-Tie2 Mouse mAb (Ab33) 試験成績書（CoA）

タイトル	ロット番号
610205	ロット番号を入力

参考資料

参考資料の概要
Lin, P., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 8829. Wong, A.L., et al. 1997. Circ. Res. 81, 567. Peters, K.G., et al. 1998. Br. J. Cancer 77, 51.

データシート

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	17-November-2017 JSW
Synonyms	Anti-Angiopoietin Receptor, Anti-Tek
Application	Frozen Sections (see comments) Immunoblotting (0.2-1 µg/ml, chemiluminescence) Immunoprecipitation (see comments) Paraffin Sections (not recommended)
Description	Protein G purified mouse monoclonal antibody. Recognizes the ~140-145 kDa Tie2 protein.
Background	Angiopoietin-1 and its naturally occurring inhibitor, angiopoietin-2, exert their effects by inducing autophosphorylation of their signaling receptor Tie2. Phosphorylation of the receptor occurs during vasculogenesis and is required for its activity in regulating endothelial cell proliferation, differentiation and guiding the proper patterning of endothelial cells during blood vessel formation. Tie-2 is a transmembrane receptor-tyrosine kinase that is expressed by endothelial cells and primitive hematopoietic tissues. Tie2/Tek has been shown to be critical for endothelial cell survival, which is evidenced in part by the constitutive phosphorylation that is observed in quiescent endothelium. More recently a multifunction docking site has been identified on the receptor (Tyr¹¹⁰⁰) that serves as a binding site for other partners including Grb2, Grb7, and p85 and acting via an SH2 domain interaction.
Host	Mouse
Immunogen species	Human
Immunogen	a recombinant protein consisting of amino acids 1-745 of human Tie2 fused to a His•Tag^® sequence
Clone	Ab33
Isotype	IgG₁
Species	human, mouse, porcine, rat
Form	Liquid
Formulation	In 150 mM NaCl, 100 mM Tris-glycine, 30% glycerol, pH 7.4.
Concentration Label	Please refer to vial label for lot-specific concentration
Preservative	≤0.1% sodium azide
Comments	For frozen sections, tissues were treated with Triton^®-X-100 detergent. This antibody has also been reported to work for immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
Storage	Avoid freeze/thaw -20°C
Do Not Freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).
Toxicity	Standard Handling
References	Lin, P., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 8829. Wong, A.L., et al. 1997. Circ. Res. 81, 567. Peters, K.G., et al. 1998. Br. J. Cancer 77, 51.

カテゴリー

Life Science Research > Antibodies and Assays > Primary Antibodies

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