04-772
Anti-Src Antibody, CT, clone NL19 | 04-772
clone NL19, Upstate®, from rabbit
別名:
Proto-oncogene tyrosine-protein kinase SRC, Protooncogene SRC, Rous sarcoma tyrosine kinase pp60c-src, Tyrosine-protein kinase SRC-1, v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
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この商品について
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
NL19, monoclonal
Application:
IP, WB
Citations:
5
biological source
rabbit
Quality Segment
conjugate
unconjugated
antibody form
culture supernatant
antibody product type
primary antibodies
clone
NL19, monoclonal
species reactivity
mouse, human, rat
manufacturer/tradename
Upstate®
technique(s)
immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... SRC(6714)
General description
SRC-1 is a member of a gene family that functions in the full transcriptional activation of the steroid hormone receptor superfamily and contains intrinsic histone acetyltransferase activity. SRC-1 has a molecular weight of 160 kDa and has a basic helix-loop-helix (bHLH)-PAS domain.
~53-62kDa
Immunogen
KLH conjugated synthetic peptide (CKVADFGLARLIEDNEYTARQGA) of Src (avian sarcoma virus), residues 400-422. The immunogen sequence shares 22/23 amino acids with Yes and Fyn, and 21/23 amino acids with Lyn and Hck.
Application
Western Blot Analysis: A 1:2000 to 1:8000 dilution of this lot detected Src in RIPA whole cell lysates from human A431 cells. Immunoprecipitation: 5-20 μL of a previous lot immunoprecipitated Src from 500 μg of A431 RIPA lysate.
Biochem/physiol Actions
Predicted to cross react with frog and chicken. The immunogen sequence shares 22/23 amino acids with Yes and Fyn, and 21/23 amino acids with Lyn and Hck.
Src-tyrosine kinase family
Physical form
Format: Purified
Analysis Note
Control
RIPA whole cell lysates from human A431, Jurkat cells, mouse 3T3/A31 cells or rat PC-12 cells Included Positive Antigen Control: Catalog # 12-301, Non-Stimulated A431 cell lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
RIPA whole cell lysates from human A431, Jurkat cells, mouse 3T3/A31 cells or rat PC-12 cells Included Positive Antigen Control: Catalog # 12-301, Non-Stimulated A431 cell lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
routinely evaluated by immunoblot on RIPA whole cell lysates from human A431 and Jurkat cells, mouse 3T3/A31 cells or rat PC-12 cells
Other Notes
Replaces: 05-772
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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保管分類
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
適用法令
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04-772:
jan
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。