Anti-Spinophilin/Neurabin-II Antibody

120 kDa

Spinophilin/neurabin II is an actin-associated scaffolding protein enriched in the dendritic spines of neurons.

GST fusion protein corresponding to residues 286-390 of rat brain spinophilin/neurabin-II.

Anti-Spinophilin/Neurabin-II Antibody is an antibody against Spinophilin/Neurabin-II for use in IC, IP & WB.

Immunoprecipitation:
4 μg of a previous lot immunoprecipitated Spinophilin/Neurabin-II from 500 μg of rat brain microsomal preparation (Catalog # 12-144).

Research Category
Neuroscience

Research Sub Category
Synapse & Synaptic Biology

Neuronal & Glial Markers

Other species not known.

Specific for spinophilin, Mr 120 kDa. Also recognizes a band which is suspected to be a cleavage product of spinophilin, Mr 95 kDa.

Format: Purified

Protein G Purified

Purified rabbit polyclonal IgG in buffer containing 70% storage buffer (0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide) and 30% glycerol. Store at -20°C.

Stable for 1 year at -20ºC from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.

Control
Brain tissue

Routinely evaluated by Western Blot on a rat brain microsomal preparation.

Western Blot Analysis:
0.25-1 μg/mL of this lot detected Spinophilin/Neurabin-II in 20 μg of a rat brain microsomal preparation (Catalog # 12-144).

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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