数量	カタログ番号	注文内容	Qty/Pk	価格
			96-Well Plate

数量	カタログ番号	注文内容	Qty/Pk	価格
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

SC1002 Sigma-AldrichAnti-Sox2 Mouse mAb (245610)

This Anti-Sox2 Mouse mAb (245610) is validated for use in Immunoblotting, Immunocytochemistry, Flow Cytometry for the detection of Sox2.

Anti-Sox2 Mouse mAb (245610): のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。

(M)SDS
試験成績書（CoA）
参考資料
Data Sheet

お勧めの製品

概要

Replacement Information

主要スペック表

Species Reactivity	Host	Antibody Type
H, M	M	Monoclonal Antibody

価格＆在庫状況

カタログ番号	在庫状況	包装	Qty/Pk	価格	数量
SC1002-100UGCN	在庫状況検索中… — 現在国内在庫なし現在国内在庫有り販売中止在庫僅少現在国内在庫あり在庫を照会するにはログインしてください現在国内在庫有り現在国内在庫なし現在国内在庫有り Remaining : Will advise Remaining : Will advise 注文対象外お問合せください Contact Customer Service お問合せくださいお問合せください	樹脂ｱﾝﾌﾟﾙ	100 μg	価格を検索中… 価格が見つかりません Minimum Quantity needs to be mulitiple of Maximum Quantity is 弊社照会詳細を表示　値引 () — 弊社照会納入価格を閲覧するにはログインしてください	在庫状況を照会 —	お気に入り/ショッピングリストに追加お気に入り/ショッピングリストに追加お気に入りに追加されました

Description
Overview	Recognizes the ~34 kDa Sox2 protein in NTERA-2 cells.
Catalogue Number	SC1002
Brand Family	Calbiochem®

References
References	Avilion, A.A., et al. 2003.Genes Dev. 17, 126. Graham, V., et al. 2003.Neuron 39, 749. Stevanovic, M. 2003.Mol. Biol. Rep. 30, 127. Kishi, M., et al. 2000.Development 127, 791. Uwanogho, D., et al. 1995.Mech. Dev. 49, 23. Yuan, H., et al. 1995.Genes Dev. 9, 2635.

Product Information
Form	Lyophilized
Formulation	Lyophilized from 0.2 µm-filter sterilized PBS, 5% trehalose.
Positive control	NTERA-2 cells
Quality Level	MQ100

Applications
Key Applications	Flow Cytometry Immunoblotting (Western Blotting) Immunocytochemistry
Application Notes	Immunoblotting (1-2 µg/ml) Immunocytochemistry (10 µg/ml; see comments) Flow Cytometry (see comments)
Application Comments	For immunocytochemistry, cells should be fixed in PBS containing 4% paraformaldehyde for 20 min, followed by blocking in PBS/10% normal donkey serum/1% BSA/0.1% Triton^® X-100 detergent for 45 min and staining overnight at 4°C with appropriately diluted primary antibody. For immunoblotting, the best results may be obtained using whole cell or nuclear extracts; Sox2 may be difficult to detect in cytoplasmic extracts. The detection limit for recombinant Sox2 is ~5 ng/lane under reducing and non-reducing conditions. For intracellular staining by flow cytometry, fix cells in 4% paraformaldehyde and permeabilize with 0.1% saponin. Following fixation and permeabilization, dilute the antibody to 50 µg/ml and add 10 µl diluted antibody to 1-2.5 x 10⁵ cells in a total volume of ≤200 µl. Following incubation with appropriate detection antibody, the cells should be washed a final time with 0.1% saponin prior to analysis. Antibody should be titrated for optimal results in individual systems.

Biological Information
Immunogen	a recombinant protein consisting of amino acids 135-317 of human Sox2, expressed in E. coli
Immunogen	Human
Clone	245610
Host	Mouse
Isotype	IgG2_a
Species Reactivity	Human Mouse
Antibody Type	Monoclonal Antibody

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Standard Handling
Storage	-20°C
Do not freeze	Ok to freeze
Special Instructions	Reconstitute with 200 µl sterile PBS for a final stock concentration of 500 µg/ml. Following reconstitution, aliquot and freeze (-20°C or -70°C) for long-term storage. Avoid freeze/thaw cycles of solutions. Thawed aliquots are stable for up to 1 month at 4°C.

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
カタログ番号	GTIN
SC1002-100UGCN	04055977209341

Documentation

Anti-Sox2 Mouse mAb (245610) (M)SDS

タイトル
英語版製品安全データシート（(M)SDS）

Anti-Sox2 Mouse mAb (245610) 試験成績書（CoA）

タイトル	ロット番号
SC1002	ロット番号を入力

参考資料

参考資料の概要
Avilion, A.A., et al. 2003.Genes Dev. 17, 126. Graham, V., et al. 2003.Neuron 39, 749. Stevanovic, M. 2003.Mol. Biol. Rep. 30, 127. Kishi, M., et al. 2000.Development 127, 791. Uwanogho, D., et al. 1995.Mech. Dev. 49, 23. Yuan, H., et al. 1995.Genes Dev. 9, 2635.

データシート

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	17-January-2008 JSW
Application	Immunoblotting (1-2 µg/ml) Immunocytochemistry (10 µg/ml; see comments) Flow Cytometry (see comments)
Description	Protein G purified mouse monoclonal antibody derived by immunizing mice with the specified immunogen and fusing splenocytes with mouse myeloma cells. Recognizes the ~34 kDa Sox2 protein.
Background	Sox2 is a member of the Sox (SRY-like HMG box) family of transcription factors, which exhibit diverse roles in development. Sox2 plays a role in specifying the first three lineages that are present at egg implantation. In addition, Sox2 functions in regulating proliferation and differentation in the developing peripheral nervous system. As a result of its role in development, Sox2, together with Oct4 and Nanog, is also essential for the propagation of undifferentiated stem cells.
Host	Mouse
Immunogen species	Human
Immunogen	a recombinant protein consisting of amino acids 135-317 of human Sox2, expressed in E. coli
Clone	245610
Isotype	IgG2_a
Species	human, mouse
Positive control	NTERA-2 cells
Form	Lyophilized
Formulation	Lyophilized from 0.2 µm-filter sterilized PBS, 5% trehalose.
Comments	For immunocytochemistry, cells should be fixed in PBS containing 4% paraformaldehyde for 20 min, followed by blocking in PBS/10% normal donkey serum/1% BSA/0.1% Triton^® X-100 detergent for 45 min and staining overnight at 4°C with appropriately diluted primary antibody. For immunoblotting, the best results may be obtained using whole cell or nuclear extracts; Sox2 may be difficult to detect in cytoplasmic extracts. The detection limit for recombinant Sox2 is ~5 ng/lane under reducing and non-reducing conditions. For intracellular staining by flow cytometry, fix cells in 4% paraformaldehyde and permeabilize with 0.1% saponin. Following fixation and permeabilization, dilute the antibody to 50 µg/ml and add 10 µl diluted antibody to 1-2.5 x 10⁵ cells in a total volume of ≤200 µl. Following incubation with appropriate detection antibody, the cells should be washed a final time with 0.1% saponin prior to analysis. Antibody should be titrated for optimal results in individual systems.
Storage	-20°C
Do Not Freeze	Ok to freeze
Special Instructions	Reconstitute with 200 µl sterile PBS for a final stock concentration of 500 µg/ml. Following reconstitution, aliquot and freeze (-20°C or -70°C) for long-term storage. Avoid freeze/thaw cycles of solutions. Thawed aliquots are stable for up to 1 month at 4°C.
Toxicity	Standard Handling
References	Avilion, A.A., et al. 2003.Genes Dev. 17, 126. Graham, V., et al. 2003.Neuron 39, 749. Stevanovic, M. 2003.Mol. Biol. Rep. 30, 127. Kishi, M., et al. 2000.Development 127, 791. Uwanogho, D., et al. 1995.Mech. Dev. 49, 23. Yuan, H., et al. 1995.Genes Dev. 9, 2635.

カテゴリー

Life Science Research > Antibodies and Assays > Primary Antibodies

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