Millipore Sigma Vibrant Logo

MABS1896-100UL Anti-RNF123 Antibody, clone 2C10.1

Anti-RNF123, clone 2C10.1, Cat. No. MABS1896, is a mouse monoclonal antibody that detects RING-type E3 ubiquitin transferase RNF123 and has been tested for use in Western Blotting.

Anti-RNF123 Antibody, clone 2C10.1: のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。
Research Category: Signaling Gene Symbol: RNF123, KPC1, PF1477 UniProt Number: Q5XPI4
View This Product on Sigma-Aldrich
MABS1896-100UL
100 µL  
価格を検索中…
価格が見つかりません
Minimum Quantity needs to be mulitiple of
Maximum Quantity is
弊社照会 詳細を表示　
値引
()
 
—
弊社照会
—
現在国内在庫なし 現在国内在庫なし
現在国内在庫有り 
販売中止
在庫僅少
現在国内在庫あり
    Remaining : Will advise
      Remaining : Will advise
      注文対象外
      お問合せください
      Contact Customer Service

       

      お問合せください

      概要

      Replacement Information

      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1896-100UL
      DescriptionAnti-RNF123 Antibody, clone 2C10.1
      Alternate Names
      • E3 ubiquitin-protein ligase RNF123
      • EC 2.3.2.27
      • Kip1 ubiquitination-promoting complex protein 1
      • RING finger protein 123
      • RING-type E3 ubiquitin transferase RNF123
      Background InformationE3 ubiquitin-protein ligase RNF123 (UniProt: Q5XPI4; also known as EC 2.3.2.27, Kip1 ubiquitination-promoting complex protein 1, RING finger protein 123, RING-type E3 ubiquitin transferase RNF123) is encoded by the RNF123 (also known as KPC1, PF1477) gene (Gene ID: 63891) in human.RNF123 serves as a catalytic subunit of the KPC complex that acts as E3 ubiquitin-protein ligase. It is required for poly-ubiquitination and proteasome-mediated degradation of CDKN1B/p27 during the cell cycle G1 phase of the cell cycle. It contains a ring-type zinc finger domain (aa 1254-1292). Two isoforms of RNF123 have been reported that are produced by alternative splicing. RNF123 is also reported to serve as a negative regulator of RIG-I and MDA5. Overexpression of RNF123 is shown to inhibit IFN-beta production triggered by Sendai virus (SeV) and encephalomyocarditis picornavirus (EMCV) and knockdown of endogenous RNF123 can potentiate IFN-beta production triggered by SeV and EMCV. RNF123 is shown to interact with the N-terminal CARD domains of RIG-I/MDA5 and compete with the downstream adaptor VISA/MAVS/IPS-1/Cardif for RIG-I/MDA5 CARD binding. (Ref.: Wang, S., et al. (2016). EMBO Rep. 17(8):1155-68).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-RNF123, clone 2C10.1, Cat. No. MABS1896, is a mouse monoclonal antibody that detects RING-type E3 ubiquitin transferase RNF123 and has been tested for use in Western Blotting.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenGST/His-tagged recombinant fragment corresponding to 92 amino acids from the N-terminal region of human RING-type E3 ubiquitin transferase RNF123.
      Clone2C10.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 2C10.1 specifically detects RING-type E3 ubiquitin transferase RNF123 in human cells. It targets an epitope within 92 amino acids from the N-terminal region.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • RNF123
      • KPC1
      • PF1477
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~150 kDa observed; 148.52 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected RING-type E3 ubiquitin transferase RNF123 in HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      MABS1896-100UL 04054839482069