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ABE2619 Anti-Polo-like kinase-1

Anti-Polo-like kinase-1, Cat. No. ABE2619, is a rabbit polyclonal antibody that detects Polo-Like Kinse 1 and has been tested for use Immunocytochemistry, Immunofluorescence, Immunoprecipitation, and Western Blotting.

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Research Category: Signaling Gene Symbol: PLK1, PLK UniProt Number: P53350
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ABE2619
100 μg  
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      概要

      Replacement Information

      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, HICC, IF, IP, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE2619
      DescriptionAnti-Polo-like kinase-1
      Alternate Names
      • Serine/threonine-protein kinase PLK1
      • EC: 2.7.11.21
      • PLK-1
      • Serine/threonine-protein kinase 13
      • STPK13
      Background InformationSerine/threonine-protein kinase PLK1 (UniProt: P53350; also known as EC: 2.7.11.21, Polo-like kinase 1, PLK-1, Serine/threonine-protein kinase 13, STPK13) is encoded by the PLK1 (also known as PLK) gene (Gene ID: 5347) in human. PLK-1 is a serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of anaphase-promoting complex/cyclosome (APC/C) inhibitors, and the regulation of mitotic exit and cytokinesis. It is shown to express in placenta and colon and its activity is enhanced by growth promoting agents. PLK-1 is activated by phosphorylation at Thr210 by aurora kinase A. It accumulates to a maximum during the G2 and M phases and declines to a nearly undetectable levels following mitosis and throughout G1 phase. However, it begins to accumulate again during the S phase of cell cycle. The kinase domain of PLK-1 is localized to amino acids 53-305 with activation loop in the region of amino acids 194-221. PLK-1 contains 2 POLO box domains (aa 417-480 and 515-584), which act as phosphopeptide-binding module that recognize and bind serine-phosphothreonine/ phosphoserine]-(proline/X) motifs. PLK-1 recognizes and binds docking proteins that are already phosphorylated on these motifs, and then phosphorylates them. Defects in PLK1 gene are associated with some cancers, such as gastric, thyroid or B-cell lymphomas. Expression is shown to be higher in tumor tissues with a poor prognosis, suggesting its role in malignant transformations and carcinogenesis.
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in PBS containing 80 mM Glycine with 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Polo-like kinase-1, Cat. No. ABE2619, is a rabbit polyclonal antibody that detects Polo-Like Kinse 1 and has been tested for use Immunocytochemistry, Immunofluorescence, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Immunofluorescence
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunofluorescence Analysis: A representative lot detected Polo-like kinase-1 in U2OS cells (Macurek, L., et. al. (2008). Nature. 455(7209):119-23).

      Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Polo-like kinase-1 in U2OS and C2C12 cells.

      Western Blotting Analysis: A representative lot detected Polo-like kinase-1 in U2OS-TR transfected cells (Macurek, L., et. al. (2008). Nature. 455(7209):119-23).

      Immunoprecipitation Analysis: A representative lot detected Polo-like kinase-1 in U2OS-TR transfected cells (Macurek, L., et. al. (2008). Nature. 455(7209):119-23).
      Biological Information
      ImmunogenHis-tagged recombinant fragment corresponding to 303 amino acids from the C-terminal half of human Polo-like kinase-1.
      EpitopeC-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects human Polo-like kinase 1. It targets an epitope within 303 amino acids from the C-terminal half.
      IsotypeIgG
      Species Reactivity
      • Mouse
      • Human
      Species Reactivity NoteHuman, Mouse.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PLK1
      • PLK
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~68 kDa observed; 68.26 kD calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in U2OS transfected with PLK1 siRNA and U2OS transfected with control oligo (LUC o Luciferase).

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected Polo-like kinase-1 in U2OS transfected with PLK1 siRNA (PLK1) & U2OS transfected with control oligo (LUC o Luciferase).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      ABE2619 04054839383663