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MABT853-25UG Anti-Podoplanin Antibody, clone LpMab-19

Anti-Podoplanin, clone LpMab-19, Cat. No. MABT853, is a mouse monoclonal antibody that detects human Podoplanin and has been tested for use in ELISA, Flow Cytometry, and Immunohistochemistry (Paraffin

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Research Category: Cell Structure Gene Symbol: PDPN, GP36 UniProt Number: Q86YL7
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MABT853-25UG
25 μg  
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      概要

      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HELISA, FC, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT853-25UG
      DescriptionAnti-Podoplanin Antibody, clone LpMab-19
      Alternate Names
      • Aggrus
      • Glycoprotein 36
      • Gp36
      • PA2.26 antigen
      • T1-alpha
      • T1A
      Background InformationPodoplanin (UniProt: Q86YL7; also known as Aggrus, Glycoprotein 36, Gp36, PA2.26 antigen, T1-alpha, T1A) is encoded by the PDPN (also known as GP36) gene (Gene ID: 10630) in human. Podoplanin serves as the endogenous ligand of C-type lectin-like receptor-2 (CLEC-2) and is highly expressed in various tumors and in some normal cells, such as lymphatic endothelial cells and podocytes. A high degree of expression is observed in placenta, lung, skeletal muscle, and brain. It may be involved in cell migration and/or actin cytoskeleton organization. Podoplanin is localized to actin-rich microvilli and plasma membrane projections, such as filopodia, lamellipodia, and ruffles. Six isoforms of podoplanin have been described that are produced by alternative splicing. It is synthesized with a signal peptide of 22 amino acids, which is cleaved to produce the mature form. Podoplanin contains an extracellular domain (aa 23-131), a helical domain (aa 132-152) and a cytoplasmic region (aa 153-162). Podoplanin possesses three tandem repeat of platelet aggregation-stimulating (PLAG) domains in its N-terminus. Among the PLAG domains, sialylated O-glycan on Thr52 of PLAG3 is essential for the binding to C-type lectin-like receptor-2 (CLEC-2). Interaction of human podoplanin with CLEC-2 mainly involves Glu47 and Asp48 in the platelet PLAG3. Clone LpMab-19 recognizes Sialylated O-Glycan on Thr76 of human Podoplanin. Its reactivity with Podoplanin is lost following T76A mutation. LpMab-19 recognizes endogenous hPDPN that is expressed in LN319 glioblastoma cell line, but fails to react with LN319 cells with hPDPN knock out. The minimum epitope of LpMab-19 is identified to be Thr76-Arg79 hPDPN. (Ref.: Ogasawara, S., et al. (2016). Monoclon. Antib. immunodiag. Immunother. 35(5); 245-253).
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Podoplanin, clone LpMab-19, Cat. No. MABT853, is a mouse monoclonal antibody that detects human Podoplanin and has been tested for use in ELISA, Flow Cytometry, and Immunohistochemistry (Paraffin).
      Key Applications
      • ELISA
      • Flow Cytometry
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Podoplanin in human colon cancer tissue.
      ELISA Analysis: A representative lot detected Podoplanin in ELISA applications (Ogasawara, S., et. al. (2016). Monoclon Antib Immunodiagn Immunother . 35(5); 245-253.
      Immunohistochemistry Analysis: A representative lot detected Podoplanin in Immunohistochemistry applications (Ogasawara, S., et. al. (2016). Monoclon Antib Immunodiagn Immunother . 35(5); 245-253.
      Flow Cytometry Analysis: A representative lot detected Podoplanin in Flow Cytometry applications (Ogasawara, S., et. al. (2016). Monoclon Antib Immunodiagn Immunother . 35(5); 245-253.
      Biological Information
      ImmunogenLN229 glioma cells expressiing human Podoplanin.
      CloneLpMab-19
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone LpMab-19 detects Podoplanin in human cells. It recognizes sialylated O-Glycan on Thr76. The minimal epitope of LpMab-19 is identified as Thr76-Arg79.
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • PDPN
      • GP36
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight16.70 kDa calculated.
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in human testis cancer tissue.

      Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected Podoplanin in human testis cancer tissue.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Global Trade Item Number
      カタログ番号 GTIN
      MABT853-25UG 04054839553608