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05-321MG Anti-Phosphotyrosine Antibody, clone 4G10®

Anti-Phosphotyrosine Antibody, clone 4G10 detects level of Phosphotyrosine & has been published & validated for use in IP & WB.

Anti-Phosphotyrosine Antibody, clone 4G10®: のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。
Research Category: Signaling Research Sub-Category: General Post-translation Modification
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05-321MG
1 mg  
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      概要

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      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      AIP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-321MG
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Phosphotyrosine Antibody, clone 4G10®
      Background InformationSome of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine.). Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity.
      The advent of anti-phosphotyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in reponse to various treatments.
      References
      Product Information
      FormatPurified
      Control
      • Untreated A-431 (negative control) and EGF treated A-431 (positive control) whole cell lysates.
        Included Positive Antigen Control: Catalog # 12-302, EGF-stimulated A431 cell lysate is provided as a free positive antigen control for western immunoblotting. Aliquot as desired, refreeze immediately, and store at -20°C. The lysate is stable for 6 months at -20°C. Before use, add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for immunoblot analysis.
      PresentationPurified mouse monoclonal IgG2bκ in buffer containing PBS, pH 7.4. Liquid at 2-8ºC.
      IgG2bκ mouse monoclonal antibody produced in vitro by mouse-mouse hybridoma 4G10® (FOX-NY [NS-1 derivative] myeloma x spleen cells).
      Quality LevelMQ100
      Applications
      ApplicationAnti-Phosphotyrosine Antibody, clone 4G10 detects level of Phosphotyrosine & has been published & validated for use in IP & WB.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation:
      2-4 μg of a previous lot immunoprecipitated quantitatively the phosphotyrosine containing proteins in the lysate of a confluent culture (10 cm dish) of cells expressing an activated tyrosine kinase. To preserve phosphotyrosine, add: 0.2 mM sodium orthovanadate to the lysis buffer.
      Biological Information
      ImmunogenPhosphotyramine-KLH
      Clone4G10®
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes tyrosine-phosphorylated proteins from all species.
      IsotypeIgG2bκ
      Species Reactivity
      • All
      Antibody TypeMonoclonal Antibody
      Modifications
      • Phosphorylation
      Purification MethodProtein G-Sepharose™ Chromatography
      Molecular WeightDependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated on EGF-treated human A431 carcinoma cells.

      Western Blot Analysis:
      0.5-2 μg/mL of this lot detected tyrosine-phosphorylated proteins in a modified RIPA lysate from EGF-treated human A431 carcinoma cells (Cohen, B., 1990; Druker, B. J., 1989; Kanakura, Y., 1991).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.

      NOTE: DO NOT FREEZE.
      For maximum recovery of the product, centrifuge the original vial prior to removing the cap. If the product has accidentally been frozen and thawed, spin it at 13,000 x g for 10 minutes at 2-8ºC. Save the supernatant for application.
      Packaging Information
      Material Size1 mg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      05-321MG 04053252370953

      Documentation

      Anti-Phosphotyrosine Antibody, clone 4G10® (M)SDS

      タイトル

      英語版製品安全データシート（(M)SDS） 

      Anti-Phosphotyrosine Antibody, clone 4G10® 試験成績書（CoA）

      タイトルロット番号
      Anti-Phosphotyrosine, clone 4G10 (mouse monoclonal IgG2bk) - DAM1400107 DAM1400107
      Anti-Phosphotyrosine, clone 4G10#174; - 2040085 2040085
      Anti-Phosphotyrosine, clone 4G10#174; - 704057853 704057853
      Anti-Phosphotyrosine, clone 4G10#174; - DAM1400107 DAM1400107
      Anti-Phosphotyrosine, clone 4G10#174; - DAM1707888 DAM1707888
      Anti-Phosphotyrosine, clone 4G10#174; - DAM1724014 DAM1724014
      Anti-Phosphotyrosine, clone 4G10#174; - DAM1747347 DAM1747347
      Anti-Phosphotyrosine, clone 4G10#174; - DAM1780352 DAM1780352
      Anti-Phosphotyrosine, clone 4G10#174; - JBC1878055 JBC1878055
      Anti-Phosphotyrosine, clone 4G10reg; - 29616 29616
      Show more

      関連製品＆アプリケーション

      Alternative Packsize

      カタログ番号 説明  
      05-321 Anti-Phosphotyrosine Antibody, clone 4G10® 価格＆在庫状況を表示
      05-321X Anti-Phosphotyrosine Antibody, clone 4G10® 価格＆在庫状況を表示

      Alternative Format

      カタログ番号 説明  
      05-777 Anti-Phosphotyrosine Antibody, recombinant clone 4G10® 価格＆在庫状況を表示
      16-101 Anti-Phosphotyrosine Antibody, clone 4G10®, agarose conjugate 価格＆在庫状況を表示
      16-103 Anti-Phosphotyrosine Antibody, clone 4G10®, Biotin Conjugate 価格＆在庫状況を表示
      16-104 Anti-Phosphotyrosine Antibody, clone 4G10®, FITC conjugate 価格＆在庫状況を表示
      16-105 Anti-Phosphotyrosine Antibody, clone 4G10®, HRP conjugate 価格＆在庫状況を表示
      16-199 Anti-Phosphotyrosine Antibody, recombinant clone 4G10®, agarose conjugate 価格＆在庫状況を表示
      16-204 Anti-Phosphotyrosine Antibody, recombinant clone 4G10®, biotin conjugate 価格＆在庫状況を表示
      16-205 Anti-Phosphotyrosine Antibody, recombinant clone 4G10®, FITC conjugate 価格＆在庫状況を表示

      Included Positive Control

      カタログ番号 説明  
      12-302 EGF-Stimulated A431 Cell Lysate 価格＆在庫状況を表示

      製品ファミリー

      ホスホチロシン　シグナリングソリューション

      Study tyrosine phosphorylation using Millipore's specific and sensitive antibodies, including the popular clone 4G10®, and new 4G10 Platinum, available in various conjugations. See below for more.
      詳細はこちら >>

      Phosphotyrosine Antibodies

      Millipore’s phosphotyrosine antibodies, including the popular 4G10 clone, demonstrate sensitivity and detect multiple tyrosine phosphorylations on numerous substrates. See below for pTyr antibodies.
      詳細はこちら >>

      カテゴリー

      Life Science Research > Antibodies and Assays > Primary Antibodies