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MABS21 Anti-PORCN Antibody, clone 15G12.1

Anti-PORCN Antibody, clone 15G12.1 is an antibody against PORCN for use in WB & IC.

Anti-PORCN Antibody, clone 15G12.1: のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。
Research Category: Signaling Research Sub-Category: Developmental Signaling Gene Symbol: PORCN, MG61, PORC, PPN UniProt Number: Q9H237
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      概要

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      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS21
      DescriptionAnti-PORCN Antibody, clone 15G12.1
      Alternate Names
      • Probable protein-cysteine N-palmitoyltransferase porcupine
      • Protein MG61
      Background InformationPORCN is a multi-pass endoplasmic reticulum protein involved in the processing of wingless and other Wnt proteins that facilitate their secretion from Wnt-producing cells. Studies have shown mutations in the genes that encode for PORCN cause a pleiotropic X-linked dominant disorder, focal dermal hypolasia (FDH, also known as Goltz syndrome). Skeletal abnormalities such as syndactyly, ectordactyly, and brachydactyly, and some cases of osteopathia striata have also been attibuted to PORCN abnormalities.
      References
      Product Information
      FormatPurified
      Control
      • Mouse brain tissue lysate
      PresentationPurified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-PORCN Antibody, clone 15G12.1 is an antibody against PORCN for use in WB & IC.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:500 dilution from a representative lot detected PORCN in HeLa and A431 cells.
      Biological Information
      ImmunogenLinear peptide corresponding to human PORCN.
      Clone15G12.1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgMκ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene belongs to the evolutionarily conserved porcupine (Porc) gene family. Genes of the Porc family encode endoplasmic reticulum proteins with multiple transmembrane domains. Porcupine proteins are involved in the processing of Wnt (wingless and int homologue) proteins. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008].
      Gene Symbol
      • PORCN
      • MG61
      • PORC
      • PPN
      UniProt Number
      UniProt SummaryFUNCTION: Modulates the processing of Wnt proteins. Probable protein-cysteine N-palmitoyltransferase that palmitoylates Wnt family members.

      SUBUNIT STRUCTURE: Interacts with WNT1, WNT3, WNT3A, WNT4, WNT5A, WNT5B, WNT6, WNT7A and WNT7B (By similarity).

      SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity).

      TISSUE SPECIFICITY: Isoform 1 is expressed in fetal brain, brain, amygdala, caudate nucleus, cerebellum, hippocampus, pituitary, thalamus, heart, skeletal muscle and testis. Isoform 4 is expressed in amygdala, corpus callosum, hippocampus, spinal cord, kidney, liver, lung, spleen, uterus, testis. Isoform 2 and isoform 3 are expressed in substantia negra, spinal cord, heart and lung.

      INVOLVEMENT IN DISEASE: Defects in PORCN are the cause of focal dermal hypoplasia (FODH) [MIM:305600]; also known as Goltz Gorlin syndrome. A rare congenital ectomesodermal disorder characterized by a combination of skin defects, skeletal abnormalities, and ocular anomalies. Affected individuals have patchy dermal hypoplasia, often in a distribution pattern following the Blaschko lines, and areas of subcutaneous fat herniation or deposition of fat into the dermis. In addition, sparse and brittle hair, hypoplastic nails and papillomas have been described. Skeletal abnormalities usually comprise syndactyly, ectrodactyly, and brachydactyly, and in some cases osteopathia striata has been seen. Patients frequently have ocular anomalies, including microphthalmia/ anophthalmia, coloboma, pigmentary and vascularization defects of the retina. Dental abnormalities are often present.

      SEQUENCE SIMILARITIES: Belongs to the membrane-bound acyltransferase family. Porcupine subfamily.

      SEQUENCE CAUTION: The sequence AAA74510.1 differs from that shown. Reason: The sequence differs from that shown upstream of position 63 for unknown reasons.
      Molecular Weight~62 kDa observed.
      Calculated molecular weight of 52 kDa. The actual MW should be observed at ~45-65 kDa. Helical membrane protein migration on SDS-PAGE may not exactly correlate with the calculated molecular weights (PNAS, 2009, 106:1760-1765).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in mouse brain tissue lysate.

      Western Blot Analysis: A 1:1,000 dilution of this antibody detected PORCN in 10 µg of mouse brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      MABS21 04053252756580

      Documentation

      Anti-PORCN Antibody, clone 15G12.1 (M)SDS

      タイトル

      英語版製品安全データシート（(M)SDS） 

      Anti-PORCN Antibody, clone 15G12.1 試験成績書（CoA）

      タイトルロット番号
      Anti-PORCN, Clone 15G12.1 - 4156863 4156863
      Anti-PORCN, clone 15G12.1 - 2450756 2450756
      Anti-PORCN, clone 15G12.1 - 2160522 2160522
      Anti-PORCN, clone 15G12.1 - 2246829 2246829
      Anti-PORCN, clone 15G12.1 - 2512194 2512194
      Anti-PORCN, clone 15G12.1 - 3290499 3290499
      Anti-PORCN, clone 15G12.1 - 3498512 3498512
      Anti-PORCN, clone 15G12.1 - NRG1945626 NRG1945626
      Anti-PORCN, clone 15G12.1 -2618351 2618351
      Anti-PORCN, clone 15G12.1 -2702186 2702186
      Show more

      参考資料

      参考資料の概要Pub Med ID
      Elevated free fatty acid uptake via CD36 promotes epithelial-mesenchymal transition in hepatocellular carcinoma.
      Nath, A; Li, I; Roberts, LR; Chan, C
      Scientific reports  5  14752  2015

      概要を表示する       		26424075 26424075