Millipore Sigma Vibrant Logo

MABS2173-100UG Anti-PNPLA3/Adiponutrin Antibody, clone 19A6

Anti-PNPLA3/Adiponutrin, clone 19A6, Cat. No. MABS2173, is a rabbit monoclonal antibody that detects PNPLA3/Adiponutrin and is tested for use in Immunoprecipitation and Western Blotting.

View This Product on Sigma-Aldrich
MABS2173-100UG
100 μg  
価格を検索中…
価格が見つかりません
Minimum Quantity needs to be mulitiple of
Maximum Quantity is
弊社照会 詳細を表示　
値引
()
 
—
弊社照会
—
現在国内在庫なし 現在国内在庫なし
現在国内在庫有り 
販売中止
在庫僅少
現在国内在庫あり
    Remaining : Will advise
      Remaining : Will advise
      注文対象外
      お問合せください
      Contact Customer Service

       

      お問合せください

      概要

      Replacement Information
      Description
      Catalogue NumberMABS2173-100UG
      DescriptionAnti-PNPLA3/Adiponutrin Antibody, clone 19A6
      Alternate Names
      • 1-acylglycerol-3-phosphate O-acyltransferase Pnpla3
      • EC:2.3.1.51
      • Acylglycerol transacylase
      • Adiponutrin
      • ADPN
      • Calcium-independent phospholipase A2-epsilon
      • iPLA2-epsilon
      • Lysophosphatidic acid acyltransferase
      • Patatin-like phospholipase domain-containing protein 3
      Background Information1-acylglycerol-3-phosphate O-acyltransferase Pnpla3 ( UniProt: Q91WW7; also known s EC:2.3.1.51, Acylglycerol transacylase, Adiponutrin, ADPN, Calcium-independent phospholipase A2-epsilon, iPLA2-epsilon, Lysophosphatidic acid acyltransferase, Patatin-like phospholipase domain-containing protein 3) is encoded by the Pnpla3 (also known as Adpn) gene (Gene ID: 116939) in murine species. PNPLA3 is a single-pass membrane glycoprotein with a cytoplasmic domain (aa 1-42), a transmembrane domain (aa 43-63), and a lumenal domain (aa 64-413). Its expression is restricted to adipose tissue and is also observed in liver in response to high-sucrose diet. It is found in hepatic lipid droplets. PNPLA3 catalyzes coenzyme A-dependent acylation of 1-acyl-sn-glycerol 3-phosphate (2-lysophosphatidic acid/LPA) to generate phosphatidic acid (PA), which is an important metabolic intermediate and precursor for both triglycerides and glycerophospholipids. For its activity, acyl donors are long chain (at least C16) fatty acyl-CoAs. PNPLA3 also exhibits low triacylglycerol lipase and CoA-independent acylglycerol transacylase activities. Mutations in Pnpla3 gene have been linked to increased risk of hepatic steatosis. Catalytically defective forms of PNPLA3 with I148M or S47A mutations are reported to cause increased hepatic triglyceride accumulation of triglycerides (TG) in sucrose fed mice. PNPLA3 knockdown is reported to reduce TG content in mice overexpressing I148 form. (Ref.: BasuRay, S., et al. (2019). Proc. Natl. Acad. Sci. USA. 116(19); 9521-9526; BasuRay, S., et al. (2017). Hepatology. 66(4); 1111-1124; Kumari, M., et al. (2012). Cell Metab. 15(5); 691-702).
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit monoclonal antibody IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-PNPLA3/Adiponutrin, clone 19A6, Cat. No. MABS2173, is a rabbit monoclonal antibody that detects PNPLA3/Adiponutrin and is tested for use in Immunoprecipitation and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesTested Applications

      Western Blotting Analysis: A representative lot detected PNPLA3 in Western Blotting applications (BasuRay, S., et al. (2017). Hepatology. 66(4): 1111-1124).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated PNPLA3 in Immunoprecipitation applications (BasuRay, S., et al. (2017). Hepatology. 66(4): 1111-1124).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenA peptide corresponding to 178 amino acids from the internal region of mouse PNPLA3/Adiponutrin.
      EpitopeInternal
      Clone19A6
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRabbit
      SpecificityClone 19A6 is a rabbit monoclonal antibody that detects 1-acylglycerol-3-phosphate O-acyltransferase Pnpla3 (PNPLA3). It targets an epitope within the internal region.
      IsotypeIgG
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Pnpla3
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~43 kDa observed; 45.77 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Mouse brain tissue extracts.

      Western Blotting Analysis: A 1:125 dilution of this antibody detected PNPLA3 in Mouse brain tissue extracts.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      MABS2173-100UG 04065268364413