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AB5509 Anti-Opioid Receptor µ Antibody

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AB5509
50 µL  
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      概要

      Replacement Information

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      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, R, PmICC, IHCGpAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB5509
      ReplacesAB1774
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Opioid Receptor µ Antibody
      Alternate Names
      • MOR-1
      References
      Product Information
      FormatAffinity Purified
      PresentationPBS containing 50% glycerol; Concentration 1.0 mg/ml
      Quality LevelMQ100
      Applications
      ApplicationAnti-Opioid Receptor μ Antibody-AB5509 is an antibody against Opioid Receptor for use in IC, IH.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      Application NotesImmunohistochemistry: 1:50-500 See Protocol.

      Immunocytochemistry: 1:50-500 See Protocol.

      Optimal working dilutions must be determined by the end user.

      IHC Protocol: Male Sprague-Dawley rats (b.wt. 100-150g) were anesthetized with sodium pentobarbital and perfused via the ascending aorta with: 1) 50 mL of Ca2+-free Tyrode+s solution followed by 2) a paraformaldehyde-picric acid fixative and 3) 10% sucrose in PBS as a cryo-protectant. Tissues were rapidly dissected out and stored overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose. Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 2-8°C for 18-24 hours with AB5509. After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.

      ICC Protocol: Mu opioid receptor transfected cells were processed for indirect immunofluorescence. Media was removed and cells were gently washed 3 times with serum free media. Media was removed and cells were gently washed 3 times with serum free media. Following fixation, cells were processed for indirect immunofluorescence as described above.
      Biological Information
      ImmunogenSynthetic peptide (Catalog number AG375) from the C-terminus of rat mu Opioid receptor corresponding to residues 384-398 of the rat mu opioid receptor.
      HostGuinea Pig
      SpecificityMu Opioid receptor.
      Species Reactivity
      • Mouse
      • Rat
      • Primate
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • OPRM1
      • MOR-1
      • MOR1
      • OPRM
      • KIAA0403
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P35372 # Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Receptor for beta-endorphin.
      SIZE: 400 amino acids; 44779 Da
      SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
      SIMILARITY: SwissProt: P35372 ## Belongs to the G-protein coupled receptor 1 family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size50 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      AB5509 04053252274626

      Documentation

      Anti-Opioid Receptor µ Antibody (M)SDS

      タイトル

      英語版製品安全データシート((M)SDS) 

      Anti-Opioid Receptor µ Antibody 試験成績書(CoA)

      タイトルロット番号
      GUINEA PIG ANTI-MU OPIOID RECEPTOR 2470353
      GUINEA PIG ANTI-MU OPIOID RECEPTOR POLYCLONAL ANTIBODY - 2392231 2392231
      GUINEA PIG ANTI-MU OPIOID RECEPTOR POLYCLONAL ANTIBODY - 2161108 2161108
      GUINEA PIG ANTI-MU OPIOID RECEPTOR - 2558351 2558351
      GUINEA PIG ANTI-MU OPIOID RECEPTOR - 3218774 3218774
      GUINEA PIG ANTI-MU OPIOID RECEPTOR - 3308262 3308262
      GUINEA PIG ANTI-MU OPIOID RECEPTOR - 3323180 3323180
      GUINEA PIG ANTI-MU OPIOID RECEPTOR - 3465515 3465515
      GUINEA PIG ANTI-MU OPIOID RECEPTOR - 3766726 3766726
      GUINEA PIG ANTI-MU OPIOID RECEPTOR - 3817609 3817609

      参考資料

      参考資料の概要Pub Med ID
      Morphine potentiates neurodegenerative effects of HIV-1 Tat through actions at μ-opioid receptor-expressing glia.
      Zou, S; Fitting, S; Hahn, YK; Welch, SP; El-Hage, N; Hauser, KF; Knapp, PE
      Brain : a journal of neurology  134  3616-31  2011

      概要を表示する
      22102648 22102648
      Activation of spinal alpha-2 adrenoceptors, but not mu-opioid receptors, reduces the intrathecal N-methyl-D-aspartate-induced increase in spinal NR1 subunit phosphorylation and nociceptive behaviors in the rat.
      Dae-Hyun Roh,Hyoung-Sig Seo,Seo-Yeon Yoon,Sunok Song,Ho-Jae Han,Alvin J Beitz,Jang-Hern Lee
      Anesthesia and analgesia  110  2010

      概要を表示する
      20007733 20007733
      The kappa-opioid receptor is primarily postsynaptic: combined immunohistochemical localization of the receptor and endogenous opioids.
      Arvidsson, U, et al.
      Proc. Natl. Acad. Sci. U.S.A., 92: 5062-6 (1995)  1995

      7539141 7539141

      データシート

      タイトル
      GUINEA PIG ANTI-MU OPIOID RECEPTOR POLYCLONAL ANTIBODY

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      カテゴリー

      Life Science Research > Antibodies and Assays > Primary Antibodies