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MAB5448 Anti-Neurofilament NF-H Antibody, clone TA51

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MAB5448
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      概要

      Replacement Information

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      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Ht, Mk, RELISA, IP, WB, IHCRCulture SupernatantMonoclonal Antibody
      Description
      Catalogue NumberMAB5448
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Neurofilament NF-H Antibody, clone TA51
      Alternate Names
      • NF-H
      References
      Product Information
      FormatCulture Supernatant
      PresentationUnpurified tissue culture supernatant from a perfusion system, filtered through a 0.2μ membrane prior to vialing. Product contains 20%FBS and Ciprofloxacin at final concentration of 10μg/mL.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Neurofilament NF-H Antibody, clone TA51 is an antibody against Neurofilament NF-H for use in ELISA, IP, WB, IH.
      Key Applications
      • ELISA
      • Immunoprecipitation
      • Western Blotting
      • Immunohistochemistry
      Application NotesWestern blot: Identifies ~ 200kDa band in reduced samples. Use Tris based buffers for best results as epitope is phosphate dependent; Different regions of the CNS were dissected out immediately and homogenized in BUST buffer (8 M urea, 0.5% SDS, 2% 13-mercaptoethanol, and 50 mM Tris, pH 7.4) at a ratio of i ml/100 mg wet tissue. The tissue suspensions were sonicated well and centrifuged in a TL-100 ultracentrifuge (Beckman Instruments, Fullerton, CA) at 100,000 g

      for 1 h at 25°C, and the protein concentrations in the supernatants were determined. Proteins were separated on 7.5% SDS-PAGE gels, {Tu. P et al (1995) J Cell Biol 129:1629-1640}

      Immunohistochemistry on frozen sections; not effective on paraffin tissues in original work. {Carden et al, 1987}.

      Optimal working dilutions must be determined by end user.
      Biological Information
      Epitopecarboxy terminal phosphorylation dependent, within the multiphosphorylation repeat in NFH
      CloneTA51
      HostRat
      SpecificityNeurofilament H (NF-H), carboxy terminal (phosphate dependent (+). Clone is specific for phosphorylated NF-H {Carden et al (1987) 7(11):3489}.
      IsotypeIgG
      Species Reactivity
      • Human
      • Hamster
      • Monkey
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NEFH
      • NFH
      • NF-H
      • KIAA0845
      Modifications
      • Phosphorylation
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P12036 # Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
      SIZE: 1026 amino acids; 112480 Da
      PTM: There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. & Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
      DISEASE: SwissProt: P12036 # Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
      SIMILARITY: SwissProt: P12036 ## Belongs to the intermediate filament family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size1 mL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      MAB5448 04053252616228

      Documentation

      Anti-Neurofilament NF-H Antibody, clone TA51 (M)SDS

      タイトル

      英語版製品安全データシート((M)SDS) 

      Anti-Neurofilament NF-H Antibody, clone TA51 試験成績書(CoA)

      タイトルロット番号
      RAT ANTI-NEUROFILAMENT H - 2829114 2829114
      RAT ANTI-NEUROFILAMENT H - 3382292 3382292
      RAT ANTI-NEUROFILAMENT H - 3384509 3384509
      RAT ANTI-NEUROFILAMENT H - 3385745 3385745
      RAT ANTI-NEUROFILAMENT H - 3440029 3440029
      RAT ANTI-NEUROFILAMENT H - 3523220 3523220
      RAT ANTI-NEUROFILAMENT H - 3587188 3587188
      RAT ANTI-NEUROFILAMENT H - 3639161 3639161
      RAT ANTI-NEUROFILAMENT H - 3848522 3848522
      RAT ANTI-NEUROFILAMENT H - 3902965 3902965

      参考資料

      参考資料の概要アプリケーションPub Med ID
      Nuclear export inhibitors avert progression in preclinical models of inflammatory demyelination.
      Haines, JD; Herbin, O; de la Hera, B; Vidaurre, OG; Moy, GA; Sun, Q; Fung, HY; Albrecht, S; Alexandropoulos, K; McCauley, D; Chook, YM; Kuhlmann, T; Kidd, GJ; Shacham, S; Casaccia, P
      Nature neuroscience  18  511-20  2015

      概要を表示する
      25706475 25706475
      Long-term maintenance of Na+ channels at nodes of Ranvier depends on glial contact mediated by gliomedin and NrCAM.
      Amor, V; Feinberg, K; Eshed-Eisenbach, Y; Vainshtein, A; Frechter, S; Grumet, M; Rosenbluth, J; Peles, E
      The Journal of neuroscience : the official journal of the Society for Neuroscience  34  5089-98  2014

      概要を表示する
      24719088 24719088
      Diffusion tensor and volumetric magnetic resonance measures as biomarkers of brain damage in a small animal model of HIV.
      Lentz, MR; Peterson, KL; Ibrahim, WG; Lee, DE; Sarlls, J; Lizak, MJ; Maric, D; Reid, WC; Hammoud, DA
      PloS one  9  e105752  2014

      概要を表示する
      25144656 25144656
      Stimulation of adult oligodendrogenesis by myelin-specific T cells.
      Hvilsted Nielsen, H; Toft-Hansen, H; Lambertsen, KL; Owens, T; Finsen, B
      The American journal of pathology  179  2028-41  2011

      概要を表示する
      Immunohistochemistry21872562 21872562
      Involvement of placental growth factor in Wallerian degeneration.
      Linda Chaballe,Pierre Close,Maxime Sempels,Stéphanie Delstanche,Julien Fanielle,Lieve Moons,Peter Carmeliet,Jean Schoenen,Alain Chariot,Rachelle Franzen
      Glia  59  2011

      概要を表示する
      21264946 21264946
      Intranuclear inclusions of expanded polyglutamine protein in spinocerebellar ataxia type 3
      Paulson, H. et al.
      Neuron, 19:333-344 (1997)  1997

      9292723 9292723
      Transgenic mice carrying a human mutant superoxide dismutase transgene develop neuronal cytoskeletal pathology resembling human amyotrophic lateral sclerosis lesions.
      Tu, P H, et al.
      Proc. Natl. Acad. Sci. U.S.A., 93: 3155-60 (1996)  1996

      概要を表示する
      8610185 8610185
      Two-stage expression of neurofilament polypeptides during rat neurogenesis with early establishment of adult phosphorylation patterns.
      Carden, M J, et al.
      J. Neurosci., 7: 3489-504 (1987)  1987

      概要を表示する
      Immunohistochemistry (Tissue)3119790 3119790

      データシート

      タイトル
      Anti-Neurofilament NF-H, carboxy terminal phosphorylation dependent, within the multiphosphorylation repeat in NFH, clone TA51 - Data Sheet

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      製品ファミリー

      カテゴリー

      Life Science Research > Antibodies and Assays > Primary Antibodies