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AB1555P Anti-NMDAR2A Antibody

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AB1555P
10 µg  
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      概要

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      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, FIHC, IP, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB1555P
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-NMDAR2A Antibody
      Alternate Names
      • N-methyl D-aspartate receptor subtype 2A
      • N-methyl-D-aspartate receptor subunit 2A
      • NMDA receptor subtype 2A
      • glutamate receptor, ionotropic
      • N-methyl D-aspartate 2A
      Background InformationNMDA (N-methyl-D-aspartate) receptors are a class of ionotropic glutamate receptors. The NMDAR channel has been shown to be involved in long term potentiation, an activity dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of the key receptor subunit NMDAR1 (GRIN1) and 1 or more of the 4 NMDAR2 subunits: NMDAR2A (GRIN2A), NMDAR2B (GRIN2B), NMDAR2C (GRIN2C), and NMDAR2D (GRIN2D).
      Product Information
      FormatAffinity Purified
      Control
      • NMDAR2A is present in high concentrations in the hippocampus.
      PresentationPurified rabbit polyclonal lyophilized from ammonium bicarbonate (5 mM) so some residual salt may also be present. Reconstitute with 50 μL of PBS. Contains no preservative.
      Quality LevelMQ100
      Applications
      ApplicationAnti-NMDAR2A Antibody is an antibody against NMDAR2A for use in IH, IP & WB.
      Key Applications
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation:
      3 μg of a previous lot was used to (under appropriate conditions) quantitatively immunoprecipitate all NMDAR2A in 200 μg of rat brain.

      Immunohistochemistry:
      A previous lot of this antibody was used at 1:1,000-1:2,000 dilution.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenC-Terminal Fusion Protein of rat NMDAR2A, aa 1253-1391.
      EpitopeC-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityNMDAR2A. By Western blot it recognizes a 180 kDa band in rat brain membranes. Does not react with NMDAR2B or NMDAR2C. Immunolabeling is blocked by preadsorption of antibody with the immunogen.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Fish
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryN-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate-gated ion channels. These receptors have been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of the key receptor subunit NMDAR1 (GRIN1) and 1 or more of the 4 NMDAR2 subunits: NMDAR2A (GRIN2A), NMDAR2B (GRIN2B), NMDAR2C (GRIN2C) and NMDAR2D (GRIN2D). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
      Gene Symbol
      • GRIN2A
      • hNR2A
      • Glutamate
      • NMDAR2A
      • NR2A
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFunction: NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation requires binding of agonist to both types of subunits.

      Subunit structure: Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B). Found in a complex with GRIN1 and GRIN3B. Found in a complex with GRIN1, GRIN3A and PPP2CB. Interacts with PDZ domains of AIP1, INADL and DLG4 By similarity.

      Subcellular location: Cell membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein.

      Sequence similarities: Belongs to the glutamate-gated ion channel (TC 1.A.10) family. [View classification]
      Molecular Weight180 kDa
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on Rat Brain Microsomal lysates.

      Western Blot Analysis: 1:500 dilution of this lot detected NMDAR2A on 10 μg of Rat Brain Microsomal lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsLyophilized material stable for 1 year at -20°C from date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up 6 months.

      Handling Recommendations:
      Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size10 µg
      Global Trade Item Number
      カタログ番号 GTIN
      AB1555P 08436037124402