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PC709 Anti-MYH (33-51) Rabbit pAb

This Anti-MYH (33-51) Rabbit pAb is validated for use in Immunoblotting, Immunofluorescence, Paraffin Sections for the detection of MYH (33-51).

Anti-MYH (33-51) Rabbit pAb: のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。

同義語: Anti-MutY Homolog

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PC709
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概要

Replacement Information

主要スペック表

Species ReactivityHostAntibody Type
H, RRbPolyclonal Antibody

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      		 ｶﾞﾗｽﾋﾞﾝ 100 ul
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      Description
      OverviewRecognizes the MYH protein and several of its approximately 10 isoforms in PC12, Jurkat, and HeLa cells.
      Catalogue NumberPC709
      Brand Family Calbiochem®
      SynonymsAnti-MutY Homolog
      References
      ReferencesWang, G., et al. 2000. Nucleic Acids Res. 28, 2135.
      Product Information
      FormLiquid
      FormulationUndiluted serum.
      Positive controlPC12, Jurkat, or HeLa cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesParaffin Sections Barouch, L.A., et al. 2006. Circ. Res. 98, 119.
      Key Applications Immunoblotting (Western Blotting)
      Immunofluorescence
      Paraffin Sections
      Application NotesImmunoblotting (1:1500)
      Immunofluorescence (1:500)
      Paraffin Sections (see application references)
      Application CommentsDetects MYH and several of its approximately ten isoforms. In Jurkat cells the ~52 kDa and ~53 kDa variants were found in the nuclear fraction while the ~57 kDa protein is found in the mitochondria. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide (QEGRQKHAKNNSQAKPSAC) corresponding to amino acids 33-51 of human MYH
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Rat
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      PC709-100ULCN 04055977207880

      Documentation

      Anti-MYH (33-51) Rabbit pAb (M)SDS

      タイトル

      英語版製品安全データシート（(M)SDS） 

      Anti-MYH (33-51) Rabbit pAb 試験成績書（CoA）

      タイトルロット番号
      PC709

      参考資料

      参考資料の概要
      Wang, G., et al. 2000. Nucleic Acids Res. 28, 2135.

      引用

      Title
    • Barouch, L.A., et al. 2006. Circulation Research 98, 119.
      		•
      データシート

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision15-October-2007 RFH
      SynonymsAnti-MutY Homolog
      ApplicationImmunoblotting (1:1500)
      Immunofluorescence (1:500)
      Paraffin Sections (see application references)
      DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~52-57 kDa isoforms of MYH.
      BackgroundOxidative DNA base lesions such as 8-oxoguanine (8-oxoG) are frequently mutagenic and have been implicated in a variety of pathophysiological states including cancer, Alzheimer's disease, arthritis, and cardiovascular disease. These lesions are generated by oxidative stress. MYH is a mammalian homolog of the E. coli Mut Y DNA glycosylase that excises adenine residues misincorporated opposite the oxidized base, 8-oxoG. MYH up-regulation can be observed following damage in mitochondrial DNA. Single mutations in this protein result in reduced translation efficiency of transcripts. Mutations in MYH have been observed in human colorectal tumors and lung cancer cell lines.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide (QEGRQKHAKNNSQAKPSAC) corresponding to amino acids 33-51 of human MYH
      IsotypeIgG
      Specieshuman, rat
      Positive controlPC12, Jurkat, or HeLa cells
      FormLiquid
      FormulationUndiluted serum.
      Preservative≤0.1% sodium azide
      CommentsDetects MYH and several of its approximately ten isoforms. In Jurkat cells the ~52 kDa and ~53 kDa variants were found in the nuclear fraction while the ~57 kDa protein is found in the mitochondria. Antibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesWang, G., et al. 2000. Nucleic Acids Res. 28, 2135.
      Citation
    • Barouch, L.A., et al. 2006. Circulation Research 98, 119.
      		•
      Application referencesParaffin Sections Barouch, L.A., et al. 2006. Circ. Res. 98, 119.