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07-641 Anti-MEK1 Antibody

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07-641
200 µg  
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      概要

      Replacement Information

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      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RIP, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number07-641
      Replaces04-376
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-MEK1 Antibody
      References
      Product Information
      FormatPurified
      Presentation70% storage buffer (0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide) and 30% glycerol.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-MEK1 Antibody is validated for use in IP, WB for the detection of MEK1.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenPeptide corresponding to amino acids 2-18 (PKKKPTPIQLNPAPDGS) of human MEK1.
      HostRabbit
      SpecificityMEK1
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NotePredicted to cross-react with rabbit based on sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.
      Gene Symbol
      • MAP2K1
      • PRKMK1
      • MKK1
      • MAPKK1
      • MEK1
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q02750 # Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.
      SIZE: 393 amino acids; 43439 Da
      SUBUNIT: Interacts with MORG1 (By similarity). Interacts with Yersinia yopJ.
      PTM: Phosphorylation on Ser/Thr by MAP kinase kinase kinases (RAF or MEKK1) regulates positively the kinase activity. & Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
      DISEASE: SwissProt: Q02750 # Defects in MAP2K1 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio- cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.
      SIMILARITY: SwissProt: Q02750 ## Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. & Contains 1 protein kinase domain.
      Molecular Weight45kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot on RIPA lysates from A431 and 3T3/A31 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      07-641 04053252621413

      Documentation

      Anti-MEK1 Antibody (M)SDS

      タイトル

      英語版製品安全データシート((M)SDS) 

      Anti-MEK1 Antibody 試験成績書(CoA)

      タイトルロット番号
      Anti-MEK1 (rabbit polyclonal IgG) - 2153576 2153576
      Anti-MEK1 (rabbit polyclonal IgG) - 2444086 2444086
      Anti-MEK1 (rabbit polyclonal IgG) 2999829
      Anti-MEK1 (rabbit polyclonal IgG) 2901521
      Anti-MEK1 (rabbit polyclonal IgG) 3091635
      Anti-MEK1 (rabbit polyclonal IgG) - 2206388 2206388
      Anti-MEK1 (rabbit polyclonal IgG) - 2309059 2309059
      Anti-MEK1 (rabbit polyclonal IgG) -2491166 2491166
      Anti-MEK1 (rabbit polyclonal IgG) -2508903 2508903
      Anti-MEK1 - 27102 27102

      参考資料

      参考資料の概要アプリケーションPub Med ID
      Anthrax lethal toxin suppresses high glucose induced VEGF over secretion through a post-translational mechanism.
      Zhang, WW; Wang, X; Xie, P; Yuan, ST; Liu, QH
      International journal of ophthalmology  8  453-8  2015

      概要を表示する
      26085990 26085990
      BCL-2 inhibition with ABT-737 prolongs survival in an NRAS/BCL-2 mouse model of AML by targeting primitive LSK and progenitor cells.
      Beurlet, S; Omidvar, N; Gorombei, P; Krief, P; Le Pogam, C; Setterblad, N; de la Grange, P; Leboeuf, C; Janin, A; Noguera, ME; Hervatin, F; Sarda-Mantel, L; Konopleva, M; Andreeff, M; Tu, AW; Fan, AC; Felsher, DW; Whetton, A; Pla, M; West, R; Fenaux, P; Chomienne, C; Padua, RA
      Blood  122  2864-76  2013

      概要を表示する
      23943652 23943652
      Anthrax lethal toxin downregulates claudin-5 expression in human endothelial tight junctions.
      D'Agnillo, F; Williams, MC; Moayeri, M; Warfel, JM
      PloS one  8  e62576  2013

      概要を表示する
      23626836 23626836
      Anthrax lethal toxin-mediated disruption of endothelial VE-cadherin is attenuated by inhibition of the Rho-associated kinase pathway.
      Warfel, JM; D'Agnillo, F
      Toxins  3  1278-93  2011

      概要を表示する 記事全文
      22069696 22069696
      MEK2 is sufficient but not necessary for proliferation and anchorage-independent growth of SK-MEL-28 melanoma cells.
      Lee, CS; Dykema, KJ; Hawkins, DM; Cherba, DM; Webb, CP; Furge, KA; Duesbery, NS
      PloS one  6  e17165  2011

      概要を表示する
      21365009 21365009
      An anthrax lethal factor mutant that is defective at causing pyroptosis retains proapoptotic activity.
      Ngai S, Batty S, Liao KC, Mogridge J
      The FEBS journal  277  119-27  2010

      概要を表示する 記事全文
      19922472 19922472
      Bacillus anthracis lethal toxin represses MMTV promoter activity through transcription factors.
      Kang, Z; Webster Marketon, JI; Johnson, A; Sternberg, EM
      Journal of molecular biology  389  595-605  2009

      概要を表示する
      19389405 19389405
      Perturbation of mouse retinal vascular morphogenesis by anthrax lethal toxin.
      Bromberg-White, JL; Boguslawski, E; Duesbery, NS
      PloS one  4  e6956  2009

      概要を表示する
      Western Blotting19750016 19750016
      Matrix metalloproteinase-activated anthrax lethal toxin demonstrates high potency in targeting tumor vasculature.
      Liu, S; Wang, H; Currie, BM; Molinolo, A; Leung, HJ; Moayeri, M; Basile, JR; Alfano, RW; Gutkind, JS; Frankel, AE; Bugge, TH; Leppla, SH
      The Journal of biological chemistry  283  529-40  2008

      概要を表示する 記事全文
      17974567 17974567
      LRP5 and LRP6 are not required for protective antigen-mediated internalization or lethality of anthrax lethal toxin.
      Young, JJ; Bromberg-White, JL; Zylstra, C; Church, JT; Boguslawski, E; Resau, JH; Williams, BO; Duesbery, NS
      PLoS pathogens  3  e27  2007

      概要を表示する
      Western Blotting17335347 17335347

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      Included Positive Control

      カタログ番号 説明  
      12-301 Non-Stimulated A431 Cell Lysate 価格&在庫状況を表示

      カテゴリー

      Life Science Research > Antibodies and Assays > Primary Antibodies