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MABC1150 Anti-MAGEA3 Antibody, clone 57B

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MABC1150
50 µL  
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      概要

      Replacement Information

      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIH(P), ICC, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABC1150
      DescriptionAnti-MAGEA3 Antibody, clone 57B
      Alternate Names
      • Antigen MZ2-D
      • Cancer/testis antigen 1.3
      • CT1.3
      • MAGE-3 antigen
      Background InformationMelanoma-associated antigen 3 (UniProt: P43357; also known as Antigen MZ2-D, Cancer/testis antigen 1.3, CT1.3, MAGE-3 antigen) is encoded by the MAGEA3 (also known as MAGE3) gene (Gene ID: 4102) in human. MAGE-3 is a member of the melanoma-associated antigen gene family of cancer antigens that are expressed in several neoplastic, but not normal, tissues with the exception of testes. In melanoma cells, it enhances viability and serves as an antigen for cytolytic T lymphocytes. MAGEA3 expression is not reported in kidney tumors, leukemias and lymphomas. MAGEA3 expression is normally limited to early developmental stages. It enhances ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. It is also reported to enhance ubiquitin ligase activity of TRIM28 and stimulate p53/TP53 ubiquitination by TRIM28. It is proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-MAGEA3 Antibody, clone 57B, Cat. No. MABC1150, detects multiple MAGE-A proteins. Validated for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Immunocytochemistry
      • Western Blotting
      Application NotesImmunocytochemistry Analysis: A representative lot detected MAGEA3 in Immunocytochemistry applications. (Hofbauer, G.F., et. al. (1997) Am J Pathol. 151(6):1549-53; Landry, C., et. al. (2000). Int J Cancer. 86(6):835-41; Kocher, T., et. al. (1995). Cancer Res. 55(11):2236-9; Juretic, A., et. al. (2003). Lancet Oncol. 4(2):104-9).

      Western Blotting Analysis: A representative lot detected MAGEA3 in Western Blot applications. (Kocher, T., et. al. (1995). Cancer Res. 55(11):2236-9; Rimoldi, D., et. al. (2000). Int J Cancer. 86(5):749-51; SHultz-Thater, E., et. al. (2011). Int J Cancer. 129(5):1137-48).

      Immunohistochemistry Analysis: A representative lot detected MAGEA3 in Immunohistochemistry applications. (Hofbauer, G.F., et. al. (1997) Am J Pathol. 151(6):1549-53; Aubry, F., et. al. (2001). Cancer. 92(11):2778-85; Cheville, J.C., et. al. (1999). Mod Pathol. 12(10):974-8; Chitale D.A., et. al. (2005). Mod Pathol. 18(1):119-26; Groeper, C., et. al. (2007). Int J Cancer. 120(2):337-43; Juretic, A., et. al. (2003). Lancet Oncol. 4(2):104-9.
      Biological Information
      ImmunogenHis-tagged recombinant human melanoma associated antigen 3 containing the polyhistidine tail.
      Epitopedomain of:
      Clone57B
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 57B specifically targets a sequence in the N-terminal region of melanoma associated antigen 3 in human testes.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MAGEA3
      • MAGE3
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight34.75 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in human testis tissue.

      Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected MAGEA3 in human testis tissue.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size50 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      MABC1150 04054839155970

      Documentation

      Anti-MAGEA3 Antibody, clone 57B (M)SDS

      タイトル

      英語版製品安全データシート((M)SDS) 

      Anti-MAGEA3 Antibody, clone 57B 試験成績書(CoA)

      タイトルロット番号
      Anti-MAGEA3, clone 57B - 3194590 3194590
      Anti-MAGEA3, clone 57B - 3305864 3305864
      Anti-MAGEA3, clone 57B - 3429609 3429609
      Anti-MAGEA3, clone 57B - 3902109 3902109
      Anti-MAGEA3, clone 57B - 4073916 4073916
      Anti-MAGEA3, clone 57B - 4108235 4108235
      Anti-MAGEA3, clone 57B - 4140422 4140422
      Anti-MAGEA3, clone 57B - 4176591 4176591
      Anti-MAGEA3, clone 57B -Q2809483 Q2809483

      参考資料

      参考資料の概要Pub Med ID
      Adoptive cancer immunotherapy using DNA-demethylated T helper cells as antigen-presenting cells.
      Kirkin AF, Dzhandzhugazyan KN, Guldberg P, Fang JJ, Andersen RS, Dahl C, Mortensen J, Lundby T, Wagner A, Law I, Broholm H, Madsen L, Lundell-Ek C, Gjerstorff MF, Ditzel HJ, Jensen MR, Fischer W
      Nature Commun. 9(1):785  2018

      概要を表示する
      29511178 29511178