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MABN2708-25UG Anti-Islet-1/2 homeobox Antibody, clone 39.4D5

Anti-Islet-1/2 homeobox, clone 39.4D5, Cat. No. MABN2708, is a mouse monoclonal antibody that detects Islet-1/2 homeobox and is tested in Western Blotting, Immunofluorescence, Immunohistochemistry, EMSA, Immunocytochemistry, ChIP, and Flow Cytometry.

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      概要

      Replacement Information
      Description
      Catalogue NumberMABN2708-25UG
      DescriptionAnti-Islet-1/2 homeobox Antibody, clone 39.4D5
      Alternate Names
      • Insulin gene enhancer protein ISL-1
      • Islet-1
      • ISL LIM homeobox 1
      • Insulin gene enhancer protein ISL-2
      • Islet-2
      • ISL LIM homeobox 2
      Background InformationInsulin gene enhancer protein ISL-1 (UniProt: P61374; also known as Islet-1) is encoded by the Isl1 gene (Gene ID: 64444) in rat. Islet-1 is a DNA-binding transcriptional activator that is predominantly localized in nucleus and, expressed in pancreatic islets and is essential for the maturation, proliferation, and survival of the endocrine pancreas. It also plays a crucial role in the development of various tissues, including the heart, motor neurons, and sensory neurons. It recognizes and binds to the consensus octamer binding site 5'-ATAATTAA-3' in promoter of target genes. Its DNA binding region is localized in amino acids 181-240. Islet-1 also contains two LIM Zinc-binding domains (aa 17-70 and 79-133) and a LIM-binding domain (aa 262-291). Two isoforms of islet-1 have been described that are produced by alternative splicing. It plays a key role in the gene regulatory network essential for retinal ganglion cell (RGC) differentiation. It cooperates with the transcription factor POU4F2 to achieve maximal levels of expression of RGC target genes and RGC fate specification in the developing retina. It is also involved in the specification of motor neurons in cooperation with LHX3 and LDB1. It is reported to be essential for cardiac development and is considered as a marker of one progenitor cell population that give rise to the outflow tract, right ventricle, a subset of left ventricular cells, and a large number of atrial cells. It also controls the expression of fibroblast growth factor and bone morphogenetic protein. Islet-1 expression is a reliable marker for pancreatic endocrine tumors and their metastases. Its expression is tightly regulated, and mutations in the ISL1 gene have been associated with congenital heart defects and developmental disorders. (Ref.: Sci Rep. 6:25928; Schick, E., et al. (2019). Sci Rep. 9(1):9358; Wang, Y., et al. (2019). Nat Commun. 10(1):4137; Bornhorst, D., et al. (2019). Nat Commun. 10(1):4113; Ma, Q., et al. (2020). Cell Rep. 31(3):107532; Lei, I., et al. (2020). Front Cell Dev Biol. 7:358; Vieira, J.R., et al. (2022). Neuron. 110(24):4074-4089; Carvelli, A., et al. (2022). EMBO J. 41(13):e108918.)
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Islet-1/2 homeobox, clone 39.4D5, Cat. No. MABN2708, is a mouse monoclonal antibody that detects Islet-1/2 homeobox and is tested in Western Blotting, Immunofluorescence, Immunohistochemistry, EMSA, Immunocytochemistry, ChIP, and Flow Cytometry.
      Key Applications
      • Western Blotting
      • Immunofluorescence
      • Immunohistochemistry
      • Electrophoretic Mobility Shift Assay
      • Immunocytochemistry
      • Chromatin Immunoprecipitation (ChIP)
      • Flow Cytometry
      Application NotesTested Applications

      Flow Cytometry Analysis: A representative lot detected Islet-1 and 2 homeobox in Flow Cytometry applications (Lei, I., et al. (2020). Front Cell Dev Biol. 7: 358).

      Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Islet-1 and 2 homeobox in Chromatin Immunoprecipitation applications (Du, A., et al. (2009). Diabetes. 58(9): 2059-69; Ma, Q., et al. (2020). Cell Rep. 31(3): 107532).

      Immunocytochemistry Analysis: A representative lot detected Islet-1 and 2 homeobox in Immunocytochemistry applications (Wang, Y., et al. (2019). Nat Commun. 10(1): 4137; Carvelli, A., et al. (2022). EMBO J. 41(13): e108918).

      Western Blotting Analysis: A representative lot detected Islet-1 and 2 homeobox in Western Blotting applications (Baleriola, J., et al. (2016). Sci Rep. 6: 25928).

      Electrophoretic Mobility Shift Assay: A representative lot detected Islet-1 and 2 homeobox in Electrophoretic Mobility Shift Assay applications (Du, A., et al. (2009). Diabetes. 58(9);:2059-69).

      Immunohistochemistry Applications: A representative lot detected Islet-1/2 homeobox in Immunohistochemistry applications (Chen, Y.Y., et al. (2007). J Neurosci. 27(31): 8448-56; Du, A., et al. (2009). Diabetes. 58(9): 2059-69; Bielle, F., et al. (2011). Neuron. 69(6):1085-98; Schick, E., et al. (2019). Sci Rep. 9(1):9358; Bornhorst, D., et al. (2019). Nat Commun. 10(1): 4113; Vieira, J.R., et al. (2022). Neuron. 110(24): 4074-4089).

      Immunofluorescence Analysis: A representative lot detected Islet-1/2 homeobox in Immunofluorescence applications (Du, A., et al. (2009). Diabetes. 58(9): 2059-69; Wang, Y., et al. (2019). Nat Commun. 10(1):4137; Vieira, J.R., et al. (2022). Neuron. 110(24): 4074-4089).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenC-terminal recombinant protein of rat Islet-1.
      EpitopeC-terminal
      Clone39.4D5
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 39.4D5 is a mouse monoclonal antibody that specifically detects Islet-1/2 homeobox. It targets an epitope within 172 amino acids from the C-terminal region.
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Frog
      • Chicken
      • Zebrafish
      Species Reactivity NoteHuman, Mouse, Rat, Frog, Chicken, Zebrafish
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Isl1
      • Isl2
      Purification MethodProtein G purified
      UniProt Number
      Molecular WeightTarget molecular weight ~43 kDa observed; 39.04 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in rat hypothalamus tissue lysate.

      Western Blotting Analysis: A 1:1000 dilution of this antibody detected Islet-1/2 homeobox in rat hypothalamus tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      MABN2708-25UG 04065272163545