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MABF2795-25UL Anti-IL-9 Antibody, clone MH9A4

Anti-IL-9, clone MH9A4, Cat. No. MABF2795, is a mouse monoclonal antibody that detects Interleukin-9 and is tested for use in ELISA and Flow Cytometry.

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MABF2795-25UL
25 µL  
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      概要

      Replacement Information
      Description
      Catalogue NumberMABF2795-25UL
      DescriptionAnti-IL-9 Antibody, clone MH9A4
      Alternate Names
      • Interleukin-9
      • Cytokine P40
      • T-cell growth factor P40
      Background InformationInterleukin-9 (UniProt: P15248; also known as IL-9, Cytokine P40, T-cell growth factor P40) is encoded by the IL9 gene (Gene ID: 3578) in human. IL-9 is a pleiotropic cytokine that plays a significant role in tolerance and inflammation. It is expressed by a variety of immune cells. It is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off to generate the mature form. It supports the growth of mast cells, T cells, and several other cell types, including erythroid progenitors, fetal thymocytes, human megakaryoblastic leukemic cells, and myeloid precursor cells. It is also reported to supports IL-2 independent and IL-4 independent growth of helper T-cells. It also plays a role in promoting IgE production by B cells. IL-9 actions are mediated via its binding to IL-9R that results in the formation the IL-9R heterocomplex. Following its binding to the IL-9R , the SHC is phosphorylated, which then binds to Grb2 that subsequently activates SoS, a GTP exchange factor for Ras GTPases. This results in the activation of Ras GTPase that activates Raf kinase followed by the activation of MEK1/2 and ERK1/2. Although IL-9 is reported to activate the MAPK pathway in several lymphoid and hematopoietic cells, this activation is weaker compared to other cytokines. Elevated levels of IL-9 and IL-9R are observed in lungs of asthmatic subjects. (Ref.: Chakraborty, S., et al. (2019). Int. J. Mol. Sci. 20(9); 2113).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-IL-9, clone MH9A4, Cat. No. MABF2795, is a mouse monoclonal antibody that detects Interleukin-9 and is tested for use in ELISA and Flow Cytometry.
      Key Applications
      • ELISA
      • Flow Cytometry
      Application NotesTested Applications

      Flow Cytometry Analysis: A representative lot detected IL-9 in Flow Cytometry applications (Lozano, E., et al. (2012). J Immunol.;188(8):3869-75; Muls, N., et al. (2012). J Neuroimmunol.;243(1-2):73-80).

      ELISA Analysis: A representative lot detected IL-9 in ELISA applications (Jenmalm, M.C., et al. (2001). Clin Exp Allergy.;31(10):1528-35; Faulkner, H., et al. (2002). J Infect Dis.;185(5):665-72; Bottcher, M.F., et al. (2003). Pediatr Allergy Immunol.;14(5):345-50; Holz, L.E., et al. (2005). J Inflamm (Lond).;2:3; Chang, H.C., et al. (2010). Nat Immunol.;11(6):527-34; Stephens, G.L., et al. (2011). Eur J Immunol.;41(4):952-62; Lozano, E., et al. (2012). J Immunol.;188(8):3869-75; Muls, N., et al. (2012). J Neuroimmunol.;243(1-2):73-80).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenOvalbumin-conjugated recombinant human Interleukin-9.
      EpitopeUnknown
      CloneMH9A4
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone MH9A4 is a mouse monoclonal antibody that detects human Interleukin-9.
      IsotypeIgG2b
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • IL9
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight15.91 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by ELISA with recombinant human IL-9.

      ELISA Analysis:: Various dilution of this antibody detected recombinant human Interleukin-9 (IL-9).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      MABF2795-25UL 04065266908121