数量	カタログ番号	注文内容	Qty/Pk	価格
			96-Well Plate

数量	カタログ番号	注文内容	Qty/Pk	価格
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

OP133 Sigma-AldrichAnti-IKKα Mouse mAb (14A231)

This Anti-IKKα Mouse mAb (14A231) is validated for use in Immunoblotting for the detection of IKKα.

Anti-IKKα Mouse mAb (14A231): のSDS（安全データシート）、CoA（試験成績書）およびCoQ（品質証明書）、カタログなど製品関連の技術資料を入手いただけます。

(M)SDS
試験成績書（CoA）
参考資料
Data Sheet

お勧めの製品

概要

Replacement Information

主要スペック表

Species Reactivity	Host	Antibody Type
H, Mk, M	M	Monoclonal Antibody

価格＆在庫状況

カタログ番号	在庫状況	包装	Qty/Pk	価格	数量
OP133-100UGCN	在庫状況検索中… — 現在国内在庫なし現在国内在庫有り販売中止在庫僅少現在国内在庫あり在庫を照会するにはログインしてください現在国内在庫有り現在国内在庫なし現在国内在庫有り Remaining : Will advise Remaining : Will advise 注文対象外お問合せください Contact Customer Service お問合せくださいお問合せください	樹脂ｱﾝﾌﾟﾙ	100 μg	価格を検索中… 価格が見つかりません Minimum Quantity needs to be mulitiple of Maximum Quantity is 弊社照会詳細を表示　値引 () — 弊社照会納入価格を閲覧するにはログインしてください	在庫状況を照会 —	お気に入り/ショッピングリストに追加お気に入り/ショッピングリストに追加お気に入りに追加されました

Description
Overview	Recognizes the ~85 kDa IKKα protein in Daudi cells.
Catalogue Number	OP133
Brand Family	Calbiochem®
Application Data	Detection of human IKKα by immunoblotting. Sample: Whole cell lysate (30 µg) from Daudi cells. Primary antibody: Anti-IKKα Mouse mAb (14A231) (Cat. No. OP133) (1 µg/ml). Detection: chemiluminescence.

References
References	Zandi, E., et al. 1998 Science 281, 1360. Didonato, J.A., et al. 1997 Nature 388, 548. Lee, F.S., et al. 1997 Cell 88, 213. Mercurio, F., et al. 1997 Science 278, 860. Regnier, C.H., et al. M. 1997 Cell 90, 373. Verma, I. and Stevenson, J.K. 1997 Proc. Nat. Acad. Sci. USA 94, 11758. Verma, I.M., et al. 1995 Genes. Dev. 9, 2723.

Product Information
Form	Liquid
Formulation	In PBS, 0.2% gelatin, pH 7.4.
Positive control	Daudi cells
Preservative	≤0.1% sodium azide
Quality Level	MQ100

Applications
Key Applications	Immunoblotting (Western Blotting)
Application Notes	Immunoblotting (1-2 µg/ml, chemiluminescence)
Application Comments	Antibody should be titrated for optimal results in individual systems.

Biological Information
Immunogen	a recombinant protein consisting of full-length human IKKα/IKK1 fused to a His•Tag^® sequence
Immunogen	Human
Clone	14A231
Host	Mouse
Isotype	IgG₁
Species Reactivity	Human Monkey Mouse
Antibody Type	Monoclonal Antibody
Concentration Label	Please refer to vial label for lot-specific concentration

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Dry Ice Only
Toxicity	Standard Handling
Storage	-20°C
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
カタログ番号	GTIN
OP133-100UGCN	04055977224801

Documentation

Anti-IKKα Mouse mAb (14A231) (M)SDS

タイトル
英語版製品安全データシート（(M)SDS）

Anti-IKKα Mouse mAb (14A231) 試験成績書（CoA）

タイトル	ロット番号
OP133	ロット番号を入力

参考資料

参考資料の概要
Zandi, E., et al. 1998 Science 281, 1360. Didonato, J.A., et al. 1997 Nature 388, 548. Lee, F.S., et al. 1997 Cell 88, 213. Mercurio, F., et al. 1997 Science 278, 860. Regnier, C.H., et al. M. 1997 Cell 90, 373. Verma, I. and Stevenson, J.K. 1997 Proc. Nat. Acad. Sci. USA 94, 11758. Verma, I.M., et al. 1995 Genes. Dev. 9, 2723.

データシート

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	02-October-2007 RFH
Application	Immunoblotting (1-2 µg/ml, chemiluminescence)
Application Data	Detection of human IKKα by immunoblotting. Sample: Whole cell lysate (30 µg) from Daudi cells. Primary antibody: Anti-IKKα Mouse mAb (14A231) (Cat. No. OP133) (1 µg/ml). Detection: chemiluminescence.
Description	Immunoaffinity purified mouse monoclonal antibody. Recognizes the ~85 kDa IKKα protein.
Background	NF-κB (nuclear factor κB) is sequestered in the cytoplasm by IκB family of inhibitory proteins that mask the nuclear localization signal of NF-κB thereby preventing translocation of NF-κB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IκB releasing NF-κB dimers. NF-κB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IκBα is autoregulated. IκB proteins are phosphorylated by IκB kinase complex consisting of at least three proteins, IKK1/α, IKK2/β and IKK3/γ. In vitro, IKK1/α and IKK2/β can form homo- and heterodimers that can phosphorylate IκBs at the regulatory serine residues directly. IKK1/α and IKK2/β are phosphorylated by NF-κB-inducing kinase (NIK) and MAP kinase kinase kinase-1 (MEKK1), respectively. Targeted disruption of IKK1/α gene in mice results in skin and limb abnormalities and death of newborns.
Host	Mouse
Immunogen species	Human
Immunogen	a recombinant protein consisting of full-length human IKKα/IKK1 fused to a His•Tag^® sequence
Clone	14A231
Isotype	IgG₁
Species	human, monkey, mouse
Positive control	Daudi cells
Form	Liquid
Formulation	In PBS, 0.2% gelatin, pH 7.4.
Concentration Label	Please refer to vial label for lot-specific concentration
Preservative	≤0.1% sodium azide
Comments	Antibody should be titrated for optimal results in individual systems.
Storage	Avoid freeze/thaw -20°C
Do Not Freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).
Toxicity	Standard Handling
References	Zandi, E., et al. 1998 Science 281, 1360. Didonato, J.A., et al. 1997 Nature 388, 548. Lee, F.S., et al. 1997 Cell 88, 213. Mercurio, F., et al. 1997 Science 278, 860. Regnier, C.H., et al. M. 1997 Cell 90, 373. Verma, I. and Stevenson, J.K. 1997 Proc. Nat. Acad. Sci. USA 94, 11758. Verma, I.M., et al. 1995 Genes. Dev. 9, 2723.

カテゴリー

Life Science Research > Antibodies and Assays > Primary Antibodies

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