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MABC1106-25UG Anti-Hsp90 alpha Antibody, clone 1G6-D7

Anti-Hsp90 alpha, clone 1G6-D7, Cat. No. MABC1106, is a mouse monoclonal antibody that detects Hsp90 alpha, Hsp90 beta and has been tested for use in ELISA, Immunoprecipitation, Functional assay, Inhibition, and Western Blotting.

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Research Category: Signaling Gene Symbol: HSP90AA1, HSP90A, HSPC1, HSPCA UniProt Number: P07900
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MABC1106-25UG
25 μg  
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      概要

      Replacement Information

      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HELISA, IP, Function Assay, Inhibition, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABC1106-25UG
      DescriptionAnti-Hsp90 alpha Antibody, clone 1G6-D7
      Alternate Names
      • Heat shock protein HSP 90-alpha
      • Heat shock 86 kDa
      • HSP 86
      • Lipopolysaccharide-associated protein 2
      • LAP-2
      • LPS-associated protein 2
      • Renal carcinoma antigen NY-REN-38
      Background InformationHeat shock protein HSP 90-alpha (UniProt: P07900; also known as Heat shock 86 kDa, HSP 86, HSP86, Lipopolysaccharide-associated protein 2, LAP-2, LPS-associated protein 2, Renal carcinoma antigen NY-REN-38) is encoded by the HSP90AA1 (also known as HSP90A, HSPC1, HSPCA) gene (Gene ID: 3320) in human. HSP90 serves as a molecular chaperone that maintains the stability and functionality of numerous client proteins in an ATPase-dependent manner. It is known to interact dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle, and chaperone function. After the completion of the chaperoning process, properly folded client protein and co-chaperone leave HSP90 in an ADP-bound partially open conformation and finally, ADP is released from HSP90 which acquires an open conformation for the next cycle. HSP90 and its co-chaperones are also shown to modulate transcription at least at three different levels (a) by altering the steady-state levels of certain transcription factors in response to various physiological cues (b) modulating the activity of certain epigenetic modifiers, such as histone deacetylases, and (c) by participation in the eviction of histones from the promoter region of certain genes and thereby turn on gene expression. In its resting state, through the dimerization of its C-terminal domain, HSP90 forms a homodimer, which is defined as the open conformation. Upon ATP-binding, the N-terminal domain undergoes significant conformational changes and comes in contact to form an active closed conformation. Following the completion of its chaperoning activity, ATP molecule is hydrolyzed to ADP which then dissociates from HSP90 and directs the protein back to the resting state. This monoclonal antibody (clone 1G6-D7) is reported to inhibit both de novo tumor formation and expansion of already formed tumors in mice. (Ref.: Li, W., et al. (2012). Biochim. Biophys. Acta. 1823(3); 730 741; Zou, M., et al. (2017). Oncogene 36(15); 2160-2171).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in PBS without azide.
      Applications
      ApplicationAnti-Hsp90 alpha, clone 1G6-D7, Cat. No. MABC1106, is a mouse monoclonal antibody that detects Hsp90 alpha, Hsp90 beta and has been tested for use in ELISA, Immunoprecipitation, Functional assay, Inhibition, and Western Blotting.
      Key Applications
      • ELISA
      • Immunoprecipitation
      • Function Assay
      • Inhibition
      • Western Blotting
      Application NotesImmunoprecipitation Analysis: A representative lot immunoprecipitated Hsp90 alpha in Immunoprecipitation applications (Dong, H., et. al. (2016). Sci Rep. 6:20605).

      ELISA Analysis: A representative lot detected Hsp90 alpha in ELISA applications (Zou, M., et. al. (2017). Oncogene. 36(15):2160-2171).

      Affects Function Analysis: A representative lot blocked parental MDA-MB-231 cell invasion in a dose-dependent manner. (Zou, M., et. al. (2017). Oncogene. 36(15):2160-2171).

      Western Blotting Analysis: A representative lot detected Hsp90 alpha in Western Blotting applications (Zou, M., et. al. (2017). Oncogene. 36(15):2160-2171).

      Inhibits Activity/Function Analysis: A representative lot inhibited both de novo tumor formation and expansion of already formed tumors in mice. (Zou, M., et. al. (2017). Oncogene. 36(15):2160-2171).
      Biological Information
      ImmunogenA recombinant fragment corresponding to 115 amino acids from the F-5 region of human HSP90 alpha.
      Clone1G6-D7
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 1G6-D7 is a mouse monoclonal antibody that detects HSP90 alpha and HSP90 beta in human cells. it targets an epitope within 9 amino acids from the internal region.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HSP90AA1
      • HSP90A
      • HSPC1
      • HSPCA
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~90 kDa observed; 84.66 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in MDA-MB-231 cell lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected Hsp90 alpha in MDA-MB-231 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      MABC1106-25UG 04054839531989