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MAB347 Anti-Growth Associated Protein 43 Antibody, clone 9-1E12

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MAB347
50 µg  
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      概要

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      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, Fe, HtIHC, IP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB347
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Growth Associated Protein 43 Antibody, clone 9-1E12
      Alternate Names
      • Neuromodulin
      • B-50 Protein
      • Growth Associated Protein 43
      • neuromodulin
      Background InformationGAP-43, a common marker of differentiating neurons, is expressed at elevated levels by developing or regenerating neurons during axon growth. While GAP-43 is an integral membrane protein associated with the cytoplasmic surface of axonal growth cones (and synapses), it is absent from dendritic growth cones. In adult brain, GAP-43 is found in high concentration in presynaptic areas where memory formation is thought to occur, such as frontal cortex, limbic system and hippocampus. Phosphorylation of GAP-43 by PKC (at Ser41 on human, mouse, rat, and bovine GAP-43 or Ser42 on chicken and Xenopus GAP-43) is specifically correlated with certain forms of synaptic plasticity.
      Product Information
      FormatPurified
      Control
      • Rat DRG tissue that has been subjected to a spinal nerve ligation and cultured neurons.
      PresentationProtein A Purified mouse immunoglobulin IgG1 in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Growth Associated Protein 43 Antibody, clone 9-1E12 detects level of Growth Associated Protein 43 & has been published & validated for use in IH, IP & WB.
      Key Applications
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunohistochemistry:
      0.1-0.5 µg/mL of a previous lot on 4% paraformaldehyde perfused and fixed rat cerebellum and cerebrum tissue. The addition of 0.01-0.1% Triton X-100 to incubation buffer will increase cellular permeability.

      Immunoprecipitation:
      A previous lot of this antibody was used in immunoprecipitation.
      5-10µg/mL in membrane preparations from 16-18 day embryonic cortical neuronal cultures.

      Western Blot Analysis:
      MAB347 will detect a single 43-48 kD band in western blots of membrane fractions of growing neurons.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenGAP-43 purified from rat brain
      Clone9-1E12
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityGAP-43 (also known as growth associated protein-43, B-50, F1 and pp46), regardless of the protein’s phosphorylation state.
      IsotypeIgG1
      Species Reactivity
      • Human
      • Rat
      • Feline
      • Hamster
      Species Reactivity NoteRat, feline and hamster.
      Expected to react with human based on sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This encoded protein is considered a crucial component of an effective regenerative response in the nervous system.
      Gene Symbol
      • GAP43
      • PP46
      • B-50
      • GAP-43/B50
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P17677 # This protein is associated with nerve growth. It is a major component of the motile growth cones that form the tips of elongating axons.
      SIZE: 238 amino acids; 24803 Da
      SUBUNIT: Binds calmodulin with a greater affinity in the absence of Ca(2+) than in its presence (By similarity).
      SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, growth cone membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, synapse. Note=Cytoplasmic surface of growth cone and synaptic plasma membranes.
      PTM: Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity.
      SIMILARITY: SwissProt: P17677 ## Belongs to the neuromodulin family. & Contains 1 IQ domain.
      Molecular Weight43-48 kDa
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on rat brain lysates.

      Western Blot Analysis:
      1:1000 dilution of this lot detected GAP-43/B-50 on 10 μg of rat brain lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C in undiluted for up to 6 months after date of receipt.
      Packaging Information
      Material Size50 µg
      Global Trade Item Number
      カタログ番号 GTIN
      MAB347 04053252725012