Anti-Glucocorticoid Receptor Antibody

Glucocorticoid receptor (UniProt P04150; also known as GR, Nuclear receptor subfamily 3 group C member 1) is encoded by the NR3C1 (also known as GCCR, GCR, GCRST, GRL) gene (Gene ID 2908) in human. Glucocorticoid receptor is widely expressed in almost all cell types and regulates genes controlling development, metabolism, and immune response upon binding glucocorticoids. Hormone-bound GR translocates from the cytoplasm to the nucleus where it regulates gene transcriptions by binding to glucocorticoid response elements (GREs) or by interacting with various transcription factors. Alternatively, hormone-bound GR can repress gene transcriptions by blocking the nuclear translocation of transcription factors. GR is composed of a modulating domain and a DNA-binding domain at its N-terminal, and a ligand-binding domain at the C-terminal.

~95 kDa observed. 85.7 kDa (Alpha; Alpha-A), 81.5 kDa (Beta; Beta-A), 85.8 kDa (Alpha-2; Gamma), 81.7 kDa (Beta-2), 64.8 kDa (GR-A alpha), 60.6 kDa (GR-A beta), 82.8 kDa (Alpha-B). 78.7 kDa (Beta-B) calculated.

Epitope: Modulating region

KLH-conjugated linear peptide corresponding to a sequence from the modulating region of human glucocorticoid receptor.

Anti-Glucocorticoid Receptor Antibody is an antibody against Glucocorticoid Receptor for use in Western Blotting, Affinity Binding Assay.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Hormones & Receptors

Western Blotting Analysis: A representative lot detected glucocorticoid receptor (GR) in mouse liver homogenates (Revollo, J.R., et al. (2013). Sci. Signal. 6(304):ra103).
Western Blotting Analysis: A representative lot detected both wild-type glucocorticoid receptor (GR) and the Dim4 DNA-binding mutant in lysates from U2OS cells that express the respective human GR constructs (Revollo, J.R., et al. (2013). Sci. Signal. 6(304):ra103).
Western Blotting Analysis: A representative lot detected both free glucocorticoid receptor (GR) and dexamethasone mesylate (Dex-Mes) labeled GR in HeLa S3 cell lysates (Cidlowski, J.A., et al. (1990). Mol. Endocrinol 4(10):1427-1437).
Affinity Binding Assay: A representative lot bound monomeric glucocorticoid receptor (GR) as well as oligomeric GR complexes in cytosolic preparations from dexamethasone-/Dex-treated HeLa S3 cells without affecting GR DNA-binding activity (Cidlowski, J.A., et al. (1990). Mol. Endocrinol 4(10):1427-1437).

Expected to react with all 10 spliced isoforms reported by UniProt (P04150-1 through P04150-10).

Rabbit polyclonal antibody in serum with 0.05% sodium azide.

Unpurified

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected glucocorticoid receptor in 10 µg of HeLa cell lysate.

Concentration: Please refer to lot specific datasheet.

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