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AB5804 Anti-Glial Fibrillary Acidic Protein (GFAP) Antibody

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AB5804
50 µL  
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      概要

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      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, Ca, H, RICC, IHC, IH(P), WBRbSerumPolyclonal Antibody
      Description
      Catalogue NumberAB5804
      ReplacesAB1540
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Glial Fibrillary Acidic Protein (GFAP) Antibody
      Alternate Names
      • glial fibrillary acidic protein
      Background InformationGlial fibrillary acidic protein is a class-III intermediate filament. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system.
      Product Information
      FormatSerum
      Control
      • Mouse brain tissue, rat astrocytes, rat neurons.
      PresentationRabbit serum. Liquid. Contains no preservative.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Glial Fibrillary Acidic Protein (GFAP) Antibody is an antibody against Glial Fibrillary Acidic Protein (GFAP) for use in IC, IH, IH(P) with more than 65 product citations.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry(paraffin):
      Representative images from a previous lot.

      Immunocytochemistry:
      A 1:1,000 dilution of a previous lot was used in Immunocytochemistry.
      Biological Information
      ImmunogenPurified bovine GFAP
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes Glial Fibrillary Acidic Protein (GFAP).
      Species Reactivity
      • Bovine
      • Canine
      • Human
      • Rat
      Species Reactivity NoteHuman, rat, bovine and canine. It is expected that the antibody will react with most mammalian GFAP due to homology. Reactivity with other species has not been confirmed.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant has been described, but its full length sequence has not been determined.
      Gene Symbol
      • FLJ45472
      • GFAP
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q28115 # GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
      SIZE: 428 amino acids; 49512 Da
      SUBCELLULAR LOCATION: Cytoplasm. Note=Associated with intermediate filaments.
      SIMILARITY: SwissProt: Q28115 ## Belongs to the intermediate filament family.
      Molecular Weight50 kDa
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunohistochemistry on brain tissue, astrocytes, and neurons.

      Immunohistochemistry(paraffin) Analysis:
      GFAP (IHC2078) staining of Human Brain. Tissue pretreated with Citrate Buffer, pH 6.0. Pre-diluted polyclonal antibody, using IHC-Select® Detection with HRP-DAB. Glial cells stain strongly (brown).
      Optimal Staining with Citrate Buffer Epitope Retrieval: Human Brain
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C undiluted aliquots from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size50 µL
      Global Trade Item Number
      カタログ番号 GTIN
      AB5804 08436037125355